98 HAIR CARE Control Water 1% Hair Shiny Fullerene® Before Before Wash X3
decoloured by treating with bleach twice. Afterwards, oxidising hair dye was applied, left for 30 minutes, shampooed, and dried with a hair dryer to create the test hair sample. As a test solution, we prepared 1% HF solution. For the experiment, once washed, dried with a towel, and treated with test solution, the experimental hair sample was dried completely with a hair dryer and sprayed lightly with water before irradiation under UVB (approximately 5mW/cm2
) for 10 minutes. Control hair After After
Figure 6: Improvement and maintenance of hair lustre by 1% Hair Shiny Fullerene.
solution or water prior to 5 minute incubation and 3 minute blow drying. The hair bundles were then arranged on cylinders of 15cm diameter, shined with light at an incidence angle of 45˚, and photographed at a position corresponding to a reflection angle of 40˚. After photographing, the hair bundles were washed with 3% sodium laureth sulfate thrice, dried with a hair dryer, and photographed again under the same condition.
Upon examination of hair lustre of the damaged hair after treatment (Fig 6), 1% HF treated damaged hair bundles showed improved hair lustre. Furthermore, even after three washes with 3% sodium laureth sulfate, the observed hair luster was evident, and as the restorative effect was still present, the hair reparative effect and persistence of γ-docosalactone were confirmed.
Suppression of hair colour fading UV-induced damage can also trigger hair colour fading. As such we also measured whether HF treatment affects colour change post-hair dyeing. White human hair was
16 14 12 10 8 6 4 2 0
nWater n 1% Hair Shiny Fullerene
samples were directly dried with a hair dryer after towel drying. This process of washing, drying, application of test solution (or non- application), and UV irradiation was counted as one cycle, and we measured hair colour with a colour-difference meter after cycles 1, 5, 10, and 20. For measurement, hair bundles were measured at six locations (front and back side of the root, middle, and end of the hair strands), averaged, and DE, the difference between hair colour laboratory values immediately after hair dying and upon hair damage, was calculated to understand how colour post- hair dyeing was affected by UV-induced damage. The results are shown in Figure 7, demonstrating that in comparison to a non- treated control, the 1% HF treated hair sample showed lower overall difference of ∆E and that fullerene nanoemulsion solution suppresses hair colour fading.
Suppression of cysteic acid production
As stated in the beginning, ROS produced from UV irradiation break cystine disulfide bonds and chemically changes them to cysteic acid. We investigated whether HF solution can suppress the formation of cysteic acid due to UV irradiation. Human black hair was first washed with 1% SDS, and then washed with water before drying naturally. Hair samples were then cut approximately at 5cm length, immobilised on a holder, and measured with infrared absorption spectrum (ATR method). Water or 5% HF solution was applied to hair samples and dried completely with a hair dryer. The hair
0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 0
1 2 10 20 (Repeat)
Figure 7: Suppression of colour change by treatment with 1% Hair Shiny Fullerene.
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n Before UV irradiation n After UV irradiation
samples were then irradiated by UV under a lamp at approximately 300J/cm2
.
Afterwards, samples were washed, dried, and measured with infrared absorption spectrum again. Cysteic acid amount was quantified by the ratio value of cysteic acid S=O absorption at 1040 cm-1 absorption at 1634cm-1 standard.
and amide , an internal As shown on the graph in Figure 8,
water treatment alone resulted in a 26% increase of S=O absorption corresponding to cysteic acid after UV irradiation, but 5% HF solution treatment showed little change. This result suggests that fullerene eliminates ROS produced from UV radiation.
Conclusion The hair care fullerene ingredient Hair Shiny Fullerene, consisting of fullerene that prevents damage resulting from ROS generated from UV or hydrogen peroxide and γ-docosalactone that repairs hair strand damage, forms nanoemulsions under a simple mixing and stirring in water protocol and exhibits superior penetration capability in hair. A series of experiments have shown that Hair Shiny Fullerene has effects ranging from cuticle protection, suppression of hair colour fading, restoration of lustre in damaged hair, and suppression of cysteic acid production. Given that Hair Shiny Fullerene is an extremely convenient raw material that can simultaneously repair existing hair damage while preventing future damage, we recommend that Hair Shiny Fullerene be added to a variety of hair care products such as non-bath hair spray and treatment. * Hair Shiny Fullerene received a Silver
award BSB Innovation Prize for Cosmetics in 2018.
Acknowledgements We thank Nippon Fine Chemical Co., Ltd for their assistance in the commercialisation of Hair Shiny Fullerene and in the collection of experimental data.
PC
References 1 Inui S, Aoshima H, Nishiyama A, Itami S. Improvement of acne vulgaris by topical fullerene application: unique impact on skin care, Nanomedicine 2011; 7(2): 238-41
2 Inui S, Mori A, Ito M, Hyodo S, Itami S. Reduction of conspicuous facial pores by topical fullerene: possible role in the suppression of PGE2 production in the skin, J Nanobiotechnology 2014; 2(6):
3 Inui S, et al. Fragrance Journal 2013; 41(11): 28-31
Control (water)
5%- Hair Shiny Fullerene®
Figure 8: Hair Shiny Fullerene suppresses cysteic acid generation by ultraviolet irradiation.
4 Takada H, Kokubo K, Matsubayashi K, Oshima T. Antioxidant activity of supramolecular water- soluble fullerenes evaluated by beta-carotene bleaching assay, Biosci Biotechnol Biochem. 2006; 70(12): 3088-93.
November 2018
∆E
A104CM*/A1634cm-1
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