26 ANTI-POLLUTION 120 100 *** 80 60 40 20 0 Control
House dust (40µg/mL)
Redivine (0.05%)
Figure 6: Redivine protects skin cells from house dust-induced cell death. Cell viability was assessed using a MTT assay. (vs. house dust: ***p-value <0.005)
treatment with Red Vine Leaf Extract prevents MMP-1 overexpression (MMP-1 production was back to basal level) and collagen is preserved (+24% hence providing a 55% protection against blue light exposure).
Domestic light exposure causes an alteration of the extra cellular matrix by decreasing collagen production while stimulating MMP-1 production which eventually leads to a loss of skin density, elasticity and flexibility. Red Vine Leaf Extract effectively negates the noxious effects of domestic light hence preventing skin premature ageing.
Conclusion
Reducing pollution is one of the foremost stakes of our modern society. In fact, pollution should be a worldwide concern as it promotes global warming and has a heavy impact on health (such as cancer, allergies, and of course premature ageing…). Besides, recent studies have shown the important impact of indoor pollution. Indeed, light from screens and lamps, heavy metals in house
a
120 100 80 60 40 20 0
Control
Blue light (30J/cm2)
* * +24% 150 100 50 0
NAC (5mM)
Redivine (0.1%)
Control
Blue light (30J/cm2)
NAC (5mM)
Redivine (0.1%)
Figure 7: Redivine prevents blue light-induced ECM alteration. Fibroblasts were exposed to blue light (30J/cm2) for 24h in the presence or in the absence of either NAC or Redivine. A) Quantification of collagen production using immunofluorescence staining. B) Quantification of MMP-1 production was measured using an ELISA assay. (vs. blue light: *p-value <0.05 ; ***p-value <0.005).
PERSONAL CARE EUROPE November 2018 *** *** -42%
dust, VOCs, etc., all contribute to affect the body, and especially skin. Indeed, skin is the first defence of the body and as such, it is the most exposed organ to ever-increasing amounts of pollution. This calls for effective daily protection. To meet this challenge, Exsymol designed an original broad-spectrum anti- pollution ingredient that fights against both environmental and indoor pollution. Redivine (INCI name: Vitis Vinifera (Grape) Leaf Extract) has a dual mode of action, providing both a direct protection by ROS scavenging, and an indirect protection by enforcing antioxidant defences of cutaneous cells (the Nrf2 pathway activation). Both properties combine to prevent DNA damages induced by a reference outdoor pollutant (benzo[a]pyrene) found in industrial exhausts, cigarette smoke, and diesel particles. For indoor pollution, Redivine properties provide protection against domestic pollutants such as lead-containing dust and artificial light (blue light). Redivine reduces lead toxicity and limits blue light- induced extrinsic ageing.
PC b 250 200 -29% -14%
References 1 Melissopoulos A, Levacher C. La peau Structure et physiologie (2e edition), editions Lavoisier 2012; 241-252
2 Costa C, Catania S, De Pasquale R, Stancanelli R, Scribano GM. Melchini A. Exposure of human skin to Benzo–a−pyrene: Role of CYP1A1 and aryl hydrocarbon receptor in oxidative stress generation, Toxicology 2010; 271: 83-86
3 Nakashima Y, Ohta S, Wolf AM. Blue light- induced oxidative stress in live skin, Free Radic Biol. Med 2017; 108: 300-310
4 Bennet D, Viswanath B, Kim S, Ahn JH. An ultra-sensitive biophysical risk assessment of light effect on skin cells Oncontarget 2017; 8 (29): 47861-47875
5 Nakatsuru Y, Wakabayashi K, Fujii-Kuriyama Y, Ishikawa T, Kusama K, Ide F. Dibenzo[A,L]pyrene-induced genotoxic and carcinogenic responses are dramatically suppressed in aryl hydrocarbon receptor-deficient mice, Int. J. Cancer 2004; 112(2): 179-183
6 Stohs SJ. Oxidative stress induced by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD), Free Radic. Biol. Med, 1990; 9(1): 79-90
7 Vomund S, Schäfer A, Parnham MJ. Brüne B, von Knothen A. Nrf2, the master regulator of anti- oxidative stress, Int. J. Mol. Sci., 2017; 18: 2772
8 Srivastava SK, HU X, Awasthi S, Amin S, Singh SV. Metabolic fate of benzo[a]pyrene-(7R,8S)- diol-(9S, 10R)-epoxide in human liver, Arch. Biochem. Biophys. 1999; 371(2): 340-344
9 Ross D, Kepa JK, winski SL, Beall HD, Anwar A, Siegel D. NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphisms, Chemico-Biol. Inter., 2000; 129: 77-97 10 Toyooka T, Ishihama M, Ibuki Y.
Phosphorylation of histone H2AX is a powerful tool for detecting chemical photogenotoxicity J. Invest. Dermatol. 2011; 131: 1313-1321
11 Liebel F, Kaur S, Ruvolo E, Kollias N, Southall MD. Irradiation of skin with visible light induces reactive oxygen species and matrix-degrading enzymes, J. Invest. Dermatol. 2012; 132: 1901-1907.
Collagen I production (% of control)
Cell viability (% of control)
MMP 1 production (% of Control)
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