ORL
ro
laringologie
Figure 3. S100 protein positive cells isolated from peritumour lymph inflammatory infiltrate (a, x400). Peritumour stroma - high density
positive cells (b, S100 protein, x400)
dermis. Non-dendritic leukocytes in the geal squamous cell carcinomas. Densi- geal carcinoma cells in patients with
dermis deep to tumour were increased ties of dendritic cells ranged from 23.0 no cervical lymph metastasis was sig-
in frequency, maximally in the dermis to 111.9, with an average of 57.2 +/- nificantly larger than those with cervi-
deep to thinner and more superficial 16.0, in the negative metastasis nodes; cal lymphatic metastasis (t = 4.889, P
melanomas. The data presented show and from 17.5 to 63.6, with an average < 0.01). Significant increase dendritic
substantial alterations in the frequency, of 38.3 +/- 14.9, in the positive metas- cells infiltration among laryngeal car-
distribution, and phenotype of LC/DC as tasis nodes (P < 0.001). Quantitative cinoma cells was found in the group
melanoma evolves, alterations that may analysis showed that patients who sur- with intensively positive reaction of
be critical for development of effective vived longer than 5 years had signifi- pericancerous lymphocyte than in the
tumour-directed immunity (19). cant higher numbers of dendritic cells medium and weakly positive groups.
Chen W.K. (9) et all. investigated the in the lymph nodes than those who The number of infiltrating dendritic
expression of S100-labeled DCs in glottic lived less than 5 years (P < 0.001). Den- cells in patients who had survived
squamous cell carcinoma, and analyzed sities of DC ranged from 32.0 to 111.9, more than 5 years was significantly
its correlation to the prognosis. The ex- with an average of 61.6 +/- 19.6, in the larger than those survived less than
pression of S100-labeled dendritic cells nodes of 20 cases of patients who sur- 5 years (t = 4.423, P < 0.01). The num-
in peritumoral tissues of 111 specimens vived longer than 5 years and from 17.5 bers of the infiltrating dendritic cells
of gllotic squamous cell carcinoma was to 65.8, with an average of 37.9 +/- 13.7, in patients with well-differentiated
detected by immunohistochemistry; 17 in the nodes of 27 cases of patients squamous cell carcinoma and poorly
specimens of normal tissues were used who survived less than 5 years. Zhang differentiated squamous cells carcino-
as control. The positive rates of S100-la- J. (10) et all. concluded that dendritic ma were 2.2 +/- 1.07 and 14.6 +/- 7.14
beled dendritic cells were 51.35% (57/111) cells are important for establishing respectively, which were significantly
in peritumoral tissues of gllotic squamous immunologic defense mechanisms in different (q = 4.532, P < 0.05). The study
cell carcinoma, and 0 in normal tissues. cases of laryngeal carcinomas, and the showed that dendritic cells infiltration
The 5-year survival rate was significantly densities of dendritic cells can serve as among laryngeal squamous carcinoma
higher in S100-positive group than in a useful indicator in assessing progno- cells played an important role in the
S100-negative group (72.31% vs. 53.91%, sis of laryngeal carcinomas. host immune reaction against tumor.
P=0.030). The glottic squamous cell carci- Feng Y. (11) et all. studied the role It indicated that when the density of
noma patients with obvious infiltration of and the clinical significance of dendrit- infiltrating dendritic cells is higher,
S100-labeled dendritic cells may achieve ic cell infiltration against tumor cells the patients had less chance of cervi-
good prognosis (9). in patients with laryngeal squamous cal lymph metastasis and may have a
Zhang J. (10) et all. performed an cell carcinoma. The relationships longer survival time. There was a coor-
immunohistochemical study to prove among the density of dendritic cell in- dinative effect between dendritic cells
dendritic cells role in immune respons- filtration in laryngeal carcinomas and infiltration in tumor cells and perican-
es against cancer. Paraffin blocks were cervical lymphatic metastasis, peri- cerous lymphocyte reaction. Thus, the
prepared for staining with antibody cancerous lymphocytic reaction and dendritic cells infiltration among la-
against S-100 proteins in 157 lymph other clinicopathologic parameters ryngeal squamous cells could be used
nodes obtained from elective cervical were observed. The numbers of den- as an index of prognosis.
lymphadenectomy in 47 cases of laryn- dritic cells infiltrating among laryn- Hu S et all. (20) studied the relation-
pag. 32
Nr. 2/februarie 2009
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