ORL
ro
Introduction
In ENT pathology, laryngeal carci-
noma represents a frequent tumour
with no predicable evolution. In the
last years several factors from prog-
nostic and therapeutic point of view,
such as tumour associated prolifera-
tion and angiogenesis, were investi-
gated. The results are still controver-
sial, and a profound tumour biology
research is needed. The majority of
these tumours are squamous cell
carcinoma(1).
Antigen presenting cells (APC)
are laryngeal tumour cells popula-
tion constant constituents. APC are
present in laryngeal stratified and
metaplasic epithelium. Their identi-
fication in laryngeal neoplasm is not
surprising. This aspect was stressed
by other authors (2).
APC dynamic reaction, their nu-
meric variability and biologic mean-
Figure 1. S100 protein positive control. Nervous fibres in transversal sections (x400)
ing are less or not known (3, 4, 5). APC
(LBAS+, DAB, modified Lillie hematoxilin).
are present in oral cavity, oesopha-
gus, anus, uterus exocollus, being dendritic cells can serve as a useful leukoplasia in 3 cases (6.12%), in situ
similar or even identical with epider- indicator in assessing prognosis of carcinoma – 1 case (2.04%), adenoid
mis Langerhans cells (6, 7). APC are laryngeal carcinomas (10). Dendritic cell carcinoma – 1 case (2,04%) and
present in oral and laryngeal squa- cells infiltration among laryngeal squamous cell carcinoma – 44 cas-
mous cell carcinoma (8). Ch en W.K. squamous carcinoma cells played an es (89,79%). Grading: G1 - 12 cases
et all. (9) investigated the expres- important role in the host immune (24,48%), G2 - 24 cases (48,97%) and
sion of S100-labeled dendritic cells reaction against tumor. Dendritic G3 - 8 cases (16,32%).
in glottic squamous cell carcinoma, cells infiltration among laryngeal Additional sections were per-
and analyzed its correlation to the squamous cells could be used as an formed for APC identification by
prognosis. The expression of S100-la- index of prognosis (11). S100 protein immunohistochemical
beled dendritic cells in peritumoral APC are identified by multiple methods (policromal, prediluted,
tissues of the majority gllotic squa- methods, but immunohistochemi- Dako, Denmark). LSAB+ represented
mous cell carcinoma specimens was cals are highly specific. Similar to working system. The final reaction
detected by immunohistochemistry. epidermis Langerhans cells, laryn- product was identified with dihidro-
The positive rates of S100-labeled geal tumour APC are moderate spec- cloride diaminobenzidin. The nucle-
dendritic cells were 51.35% in peri- ificity S100 protein constant positive us was stained with modified Lillie
tumoral tissues of gllotic squamous cells and high specificity CD21 (12, hematoxilin. For avoiding APC nu-
cell carcinoma, and 0 in normal tis- 13). For these reasons we investigat- meric overestimation we performed
sues. The 5-year survival rate was ed, by testing S100 protein reactivity, CD68 macrophages specific immu-
significantly higher in S100-positive laryngeal tumours APC presence and noreactions. APC like macrophages
group than in S100-negative group. prognostic role. subpopulations may express nuclear
The glottic squamous cell carcinoma and cytoplasm S100 protein. Stroma
patients with obvious infiltration Material and methods nervous fibres represented S100 pro-
of S100-labeled dendritic cells may 49 laryngeal tumours were retrospec- tein immunoreaction positive con-
achieve good prognosis. tively analysed. Treatment consisted in trol (Fig. 1). The immunohistochemic
Dendritic cells may be more di- performing total laryngectomy, fron- procedure had been performed in
rectly involved in the host immune tolateral laryngectomy or endoscopic automated system, with DakoCyto-
reaction against tumor by means CO2 Laser tumour excision. mation (Dako, Denmark).
of the most important antigen-pre- We applied usual histological tech- Evaluation of S100 protein positive
senting cells. Dendritic cells in the nique, fixation in formalin and inclu- cell density has been performed in
cervical lymph nodes are important sion in paraffin. For histopathologic tumour and peritumour area using
for establishing immunologic de- diagnosis and modified Broders hot-spot method, at x400 magnifica-
fense mechanisms in cases of laryn- system grading we used Hematoxi- tion. Only positive cells, with identi-
geal carcinomas, and the densities of lin Eozin staining. We encountered fiable body cell, were countered; iso-
Nr. 2/februarie 2009
pag. 29
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