ANTI-AGEING 1.6
1.55 1.5
1.45 1.4
1.35 1.3
0 days 14 days 28 days
Figure 5a: Displacement from Phi value obtained before and after 14 and 28 days of product application in the face. Statistical significance: * p < 0.05 calculated using unpaired t test
and processed with software to generate colormap images. With treatment with the Bacillus Ferment, there was an increase in skin luminosity of 13.91% after 14 days of product application. The uniformity of skin luminosity across
the left and right sides of the face was calculated. A reduction in the difference of the skin luminosity between the left and right cheeks was observed over the same time period, demonstrating a more uniform luminosity across the face after using the cream containing Bacillus Fermen.
Discussion As found in the results of this study, we have developed a new protocol to successfully isolate and culture TCs from human skin. Isolated cells showed the typical morphology for this cell type with small cell body and several cellular prolongations described by others.6,9 As stated by Cefalan et al.,6
the
main difficulty in identifying TCs with light microscopy is that they are morphologically similar to different types of so called ‘fibroblast-like’ or ‘dendritic- like’ stromal cells. Therefore, we used triple immunostaining with specific markers to further confirm the identification of TCs.9,12 In this study a high dose of cortisol, the
primary stress hormone, has been used for the first time on skin TCs cultures to mimic stress conditions. Our observations support the notion that stress can have a negative impact on skin cells including TCs. This reveals a new mechanism by which
long-term chronic stress can accelerate the ageing process and can lead to premature skin ageing. The newly developed Bacillus Ferment showed to prevent cortisol-induced damage to protect reductions in TC number and communication through exosome release. We have also developed a new system
to quantify facial harmony that draws on the mathematical expression of Phi. According to Milutinovic et al.,13
the beauty
of a person’s face is determined by the harmony of proportions and symmetry, and ideal proportions are directly related to Phi proportions. Other authors showed the relation of
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Figure 5b: After 28 d of treatment with the Bacillus Ferment, facial proportions were brought closer to the Phi ratio, revealing a more harmonious facial appearance
this divine proportions to beauty in several populations.14,15
The aim of the clinical trial
done in this investigation was to evaluate if a treatment able to protect TCs and their function could counteract the deviation of Phi proportions observed in volunteers under psychological stress. A deviation of facial proportions from the Phi number in volunteers suffering psychological stress was determined. The ratio was 25% closer to Phi after 28 days treatment with a cream containing 2% of the Bacillus Ferment, revealing a more harmonic facial appearance. Facial skin firmness, oval contour and luminosity also increased and became more homogeneous after 28 days of product application.
Conclusion Understanding how psychological stress can impact the skin may help to uncover how skin appearance can change during periods of prolonged stress. While factors like dullness and wrinkles may be alleviated with standard cosmetic active ingredients, a more targeted approach to reduce the damage caused to TCs may be required to address morphological changes that can occur in the skin like facial asymmetry. We have identified a new mechanism
by which psychological stress can damage the skin, and demonstrated that the new biotechnological active ingredient, Telophi, can revert this damage. With Telophi biotech ingredient, facial harmonic proportions can be improved, helping the skin approach the Phi ratio and can improve firmness, oval contour, and luminosity in a homogeneous and symmetrical manner. Telophi biotech ingredient is ideal for daily
facial skincare products that protect the skin from the damage caused by psychological stress and for cosmetic formulations that improve skin appearance and restore one’s own natural beauty.
References 1. Chen Y, Lyga J. Brain-Skin Connection: Stress, Inflammation and Skin Aging. Inflamm. & Allergy - Drug Targets. 2014; 13(3):177-190
2. Tower J. Stress and stem cells. Wiley Interdiscip. Rev. Dev. Biol. 2012; 1(6):789-802
3. Lee CM, Watson REB, Kleyn CE. The impact of perceived stress on skin ageing. J Eur Acad Dermatol Venereol. 2020; 34(1):54-58
4. Egawa M, Haze S, Gozu Y, Hosoi J, Onodera T, Tojo Y, Katsuyama M, Hara Y, Katagiri C, Inoue N, Furukawa S, Suzuki G. Evaluation of psychological stress in confined environments using salivary, skin, and facial image parameters. Sci Rep. 2018; 8:8264
5. Rusu MC, Mirancea N, Mănoiu VS, Vâlcu M, Nicolescu MI, Păduraru D. Skin telocytes. Ann Anat. 2012; 194:359-367
6. Ceafalan L, Gherghiceanu M, Popescu LM, Simionescu O. Telocytes in human skin-are they involved in skin regeneration? J. Cell. Mol. Med. 2012; 16(7):1405-1420
7. El Maadawi ZM. A tale of two Cells: Telocyte and Stem Cell Unique Relationship. Adv. Exp. Med. Biol. 2016; 913:359-376
8. Li YY, Zhang S, Li YG, Wang Y. Isolation, culture, purification and ultrastructural investigation of cardiac telocytes. Mol. Med. Rep. 2016; 14:1194- 1200
9. Manole CG, Simionescu O. The Cutaneous Telocytes. Adv. Exp. Med. Biol. 2016; 913:303- 323
10. Bashour M. History and current concepts in the analysis of facial attractiveness. Plast. Reconstr. Surg. 2006; 118(3):741-756
11. Schmid K, Marx D, Samal A. Computation of a face Attractiveness Index Based on Neoclassical cannons, symmetry, and golden ratios. Pattern Recognit. 2008; 41:2710-2717
12. Wang L, Song D, Wei C, Chen C, Yang Y, Deng X, Gu J. Telocytes inhibited inflammatory factor expression and enhanced cell migration in LPS-induced skin wound healing models in vitro and in vivo. J. Transl. Med. 2020; 18(1):60
13. Milutinovic J, Zelic K, Nedeljkovic N. Evaluation of Facial Beauty Using Anthropometric Proportions. Sci. World J. 2014; 2014:428250
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14. Anand S, Tripathi S, Chopra A, Khaneja K, Agarwal S. Vertical and horizontal proportions of the face and their correlation to phi among Indians in Moradabad population: A survey. J. Indian Prosthodont. Soc. 2015; 15(2):125-30
15. Mantelakis M, Al-Bitar ZB, Antoniadis V, Wertheim D, Garagiola U, Naini FB. Proportions of the aesthetic African-Caribbean face: idealized ratios, comparison with the golden proportion and perceptions of attractiveness. Maxillofac. Plast. Reconstr. Surg. 2018; 40(1):20
September 2022 PERSONAL CARE *
Bacillus Ferment cream■ Placebo■ Φ (Phi)
* D0 D28
83
Displacement from phi value
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