ANTI-AGEING
Novel active ingredient to restore facial harmony
Bianca McCarthy, Gemma Mola, Nuria Almiñana, Raquel Delgado – Lubrizol Life Science
Chronic psychological stress has a detrimental impact on the human body, including the skin. Cortisol can activate biological pathways that cause inflammation, oxidative stress, and DNA damage,1,2 to signs of premature ageing,3
leading including lack
of radiance and a tired appearance. Recent evidence suggests that the natural
symmetry of facial proportions can also be altered by psychological stress.4
Yet the
mechanism of action by which stress can alter facial balance remains unknown. The skin morphological changes reported
after long periods of stress points to the role of cells responsible for skin architecture. In 2012, a new cell type was discovered in skin called telocytes (TCs) meaning ‘cells bearing long prolongations’.5
moniliform, and occasionally convoluted prolongations make TCs different from other stromal cell types such as fibroblasts, fibrocytes, or fibroblast-like cells. Among other roles, TCs are thought to
provide mechanical support throughout the tissue,6,7
and can also participate in the
regulation of stem cells via the release of extracellular vesicles, also known as exosomes. However, due to challenges in isolation and characterization – indeed, TCs are not commercially available – it is unclear whether TC functionality is impacted by cortisol. Therefore, the aim of this study was to
firstly, determine the effect of cortisol in skin TCs and secondly, how these negative effects could be minimized with Telophi™ biotech ingredient to restore facial harmony for an
120 100 80 60 40 20 0
inclusive beauty solution to counteract the impact of stress on facial appearance.
Successful isolation and identification of TCs from human skin biopsies To gain relevant insights into the possible functions of TCs, we have developed a new protocol to isolate and culture TCs from human skin, based on previous work described by Li et al. with cardiac TCs from
rats.8 This new protocol included a specific
step to eliminate fibroblasts from the cell culture to remove any morphologically similar cell types. Isolated cells were visually characterized with a small cell body and long moniliform telopodes (Figure 1). TCs were confirmed by performing a triple immunocytochemistry step to detect the three markers - CD34, PDGFRa and vimentin (Figure 1) – reported to be characteristic to TCs.8,9
83.3%
73.2% ****
65.9% ****
47.3% ****
Cortisol can reduce the number of TCs and their exosome release Using TCs isolated following the newly established protocol, we studied the effect of stress by adding cortisol to the cell culture to mimic psychological stress conditions. This is the first reported study looking at the role of psychological stress in TCs. Cell cultures of skin TCs were treated with
Control 0.4 mM cortisol 0.6 mM cortisol 0.8 mM cortisol 1 mM cortisol
Figure 2: Telocyte number, as quantified as the mean percentage of double positive cells (CD34/ PDGFRa) in TCs culture after cortisol treatment compared to control condition (CTR). Statistical significance: ****p < 0.0001 using unpaired t test
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cortisol at different concentrations (0.4 mM, 0.6 mM, 0.8 mM and 1 mM). We found that cortisol can have a negative impact on TCs: it can significantly reduce the number of TCs, showing a maximum reduction in TCs number at 1 mM.
The highest tested concentration of
cortisol (1 mM) was selected to test if the reduction of TCs number could be recovered
September 2022 PERSONAL CARE B Indeed, these long, thin, A
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Figures 1a and 1b: Triple immunofluorescent staining for CD34/PDFGRa/vimentin of skin TCs in primary culture
Telocytes number (%)
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