SKIN MICROBIOME
cytokines by inhibiting their metabolic routes of production.18
In vitro tests The biotic ingredient contributes to achieve eubiosis stage by favouring S. epidermidis and controlling the S. aureus and C. acnes populations.
Prebiotics nourishing Staphylococcus epidermidis populations and inhibiting Staphylococcus aureus populations A co-culture in vitro test of microbial competition between S.epidermidis vs
S.aureus strains was performed. Results showed an increase of +21% in S.epidermidis populations against
S.aureus ones (Figure 4).
Prebiotics decreasing C. acnes populations An in vitro assay was conducted under anaerobic conditions on the microbial strain C. acnes phylotype IA1. Results indicated a statistically significant
99.9% inhibition of
C.acnes populations when incubated with the biotic ingredient at a dose of 0.5% (Figure 5). The biotic ingredient restores the balance of the microbiota by controlling
C.acnes populations.
Postbiotics suppressing DHT production by inhibiting 5α-reductase Inhibition of 5α-reductase by ELISA assay was tested using 0.001% of the biotic ingredient. The biotic ingredient inhibited 5α-reductase by 28.8%±3.5%. The biotic ingredient is able to down-
regulate the production of DHT, by inhibiting the enzyme 5α-reductase, and then, controlling sebum production.
Clinical efficacy of the biotic ingredient for acne prone skin The biotic ingredient was assessed in a double- blind clinical study to test its effectiveness in reducing acne. The study was conducted with 40 volunteers with acne-prone skin. The age range was between 19 and 29 years old. They applied SYN BIO PSK 1% cream (20
volunteers) or placebo cream (20 volunteers) to the face, twice a day for 28 days. The biotic ingredient reduced sebum by -37% and increased moisture by +68%. Visible changes can be observed in Figure 6.
0
-10 -20 -30 -40 -50 -60 -70
C-
Figure 6: Clinical study of the biotic ingredient 1%. Visual skin injuries reduction (sebum, redness and bright reduction) after 28 days of usage
Clinical study: the biotic ingredient as pore size minimizer The biotic ingredient was assessed in a double-blind clinical study to assess its potential to reduce facial pores. The study was conducted with 20 volunteers with large pore sizes and between 20 and 30 years of age. One group used the biotic ingredient at 1%
on the face, while the placebo group used only a base cream without the biotic ingredient. They applied the cream twice a day for 28 days.
The biotic ingredient significantly reduced -43% pore area *
Biotic ingredient 1%
Figure 7: Medium pore area reduction. Placebo/ Active. *p<0.05; Kruskall Wallis test. Image measurements with Image J software
www.personalcaremagazine.com
50 40 30 20 10 0
Placebo
+30.4% Facial skin smoothed
*
the area of facial pores by - 43% compared to the placebo group. The absolute value of pore size reduction in the active group was -46.35% compared to the weak reduction in the placebo group of only -3.88% (Figure 7). Additionally, the biotic ingredient
showed a significant effect on increasing the smoothed facial skin area by +30.4% comparing the active group with the placebo group (Figure 8). Visual reduction of pore size is depicted in Figure 9. The biotic ingredient was able not only
to reduce facial pores but also to produce an outstanding increase in moisture and a sebum reduction, reducing acne in oily- prone skin. Pore size is positively correlated to sebum overproduction; hence, the biotic ingredient acts as a pores minimizer thanks to the downregulation of sebum production.
Biotic ingredient 1%
Figure 8: Smoothed facial skin area (%). Placebo/Active. *p<0.05; Kruskall Wallis test. Image measurements with Image J software
Formulating with the biotic ingredient The biotic ingredient contains a unique blend of prebiotic and postbiotic molecules produced by phytoplankton and its microbiota and extracted in an environmentally friendly downstream process. This biotic ingredient is water dispersible, 100% natural origin content (ISO 16128), vegan and COSMOS-certified. Additionally, it is Halal-certified and China compliant.
May 2024 PERSONAL CARE
0.16 0.12 0.08 0.04 0
NC
+21% S.epidermidis **
77
Biotic ingredient 0.05%
Figure 4: In vitro co-culture microbial competence 24h; 37°C. cfu = colony-forming unit; s.e = Staphylococcus epidermidis; s.a. = Staphylococcus aureus. T-student test; **p-value<0.01
DAY 0
120 100 80 60 40 20 0
NC
-99% Cutibacterium acnes
***
Biotic ingredient 0.05%
Figure 5: In vitro culture Cutibacterium acnes in anoxic conditions. 24h; 37°C. ***p-value<0.001.; T-student test
DAY 28
Facial pores area (%)
Smoothed facial skin area (%)
S.e./ S.a. ufc ratio
Cell viability (%)
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