SKIN MICROBIOME
TABLE 2: ANTI-MICROBIAL SENSITIVITY OF SPECBIO LF SERIES Bacteria/Fungi
Inhibition zone diameter (mm)
Staphylococcus aureus Escherichia coli
Pseudomonas aeruginosa Staphylococcus epidermidis Candida albicans Aspergillus niger
Propionibacterium acnes Malassezia furfur
Sterile deionized water (blank control)
None 9.49
None None 12.8
10.36 None 12.73 None
65
Sample Name SpecBio LF Hydrogel Sensitivity
Insensitive Low
Insensitive Insensitive Medium Medium
Insensitive Medium
Insensitive SpecBio LF Filtrate
Inhibition zone diameter (mm)
None None None None None None None 10.84 None
Sensitivity
Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive Medium
Insensitive Reference Product 1
Inhibition zone diameter (mm)
None None None None None None None None None
Sensitivity
Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive Insensitive
Sensitivity is determined based on the diameter of the inhibition zone. Inhibition zone diameter = 0mm, Insensitive; 0 < inhibition zone diameter < 10mm, low sensitivity; 10mm≤ If the diameter of the inhibition zone is <15mm, medium sensitivity; if 15mm≤the diameter of the inhibition zone is <20mm, high sensitivity; if 20mm≤the diameter of the inhibition zone, extreme sensitivity
A
80 60 40 20 0
B
100 80 60 40 20 0
DPPH Scavenging
Blank Medium ■ Ferment w/o TCM ■ Reference Product 1 ■ SpecBio LF Filtrate ■ Ferment w/o TCM HG ■ SpecBio LF HG ■
rate of NIH/3T3 cells by around 100% after 24 hours treatment, indicating their strong repairing potentials against various cell damages (Figure 4). Interestingly, from the single-fibre contact
0 100 200 300 400 Concentration (mg/ml) Superoxide Anion Radical Scavenging 500 600 700
angle measurement, a method showing the change to the surface of damaged hair, the LF filtrate was also found to be able to repair the damaged hair. Figure 5 shows the result of contact angle
measurement after treating mildly damaged hairs with various ingredients. After treatment with the LF filtrate, the wettability of the damaged hair is comparable to those treated with some hydrolyzed proteins (Figure 5).
0 50 100 150 200 Concentration (mg/ml)
Figure 3: Scavenging of (A) DPPH radicals and (B) superoxide anion radicals by SpecBio LF. TCM: traditional Chinese medicinal plant extracts, HG: hydrogel
A Control
SpecBio LF Hydrogel 0.50 mg/mL
SpecBio LF Filtrate 0.50 mg/mL
250 300 350
Hydrogel technology improved homogeneity for Lactobacillus ferment lysates One of the most common problems in the market regarding Lactobacillus ferment lysates is product precipitates (Figure 6A), which has become a headache for many cosmetic manufacturers in subsequent production. SpecChem’s LF Lactobacillus ferment
B 0h
250 200 150 100 50 0
* * *
SpecBio LF Hydrogel 24h Concentrations (mg/mL)
24h
250 200 150 100 50 0
*
SpecBio LF Filtrate 24h Concentrations (mg/mL)
Figure 4: Wound healing assay in NIH/3T3 cells treated with SpecBio LF Hydrogel and SpecBio LF Filtrate. A: Representative images of wound closure. B: Relative mobility of NIH/3T3 cells at 24 hours ( *p<0.05 versus control)
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% of Scavenging
% of Scavenging
Relative Mobility (%)
Relative Mobility (%)
Control 0.50 0.25 0.13 0.06 Control 0.50 0.25 0.13 0.06
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