72 MARINE INGREDIENTS
RHE for 18 hours. The viability of tissues was evaluated by a MTT test, and the inflammatory response was assessed by interleukin 1 alpha (IL- 1a) release. The inflammatory response (IR) was expressed as: IR = IL-1a (treated) / IL-1a (negative control) No significant cytotoxicity (tissue
viability=99%) was recorded after topical application ofNannochloropsis extract (Figure 10). Under the same experimental conditions, 0.5% of SLS solution induced a complete mortality of tissues, as well as with LA (viability 9%).
This result demonstrates the increased
sensitivity of immature RHE, since the viability of standard mature RHE, treated with LA, is always ≥60%. The Nannochloropsis extract is totally non-cytotoxic on a baby skin model. Results evidenced that the inflammatory
response (IR) of tissues treated with the Nannochloropsis extract (IR=2) was not or only weakly modified compared to negative control tissues (IR=1), this difference is not significant (Figure 11). The topical application of LA induced a slight
increase of the inflammatory response of tissues (IR=7). The topical application of SDS (0.5%) induced a maximal IL-1a release, related to the drastic reduction of tissue viability. To be considered of good tolerance on baby
skin, the tested item should answer to both criteria : good cell viability and no inflammatory response. In this evaluation, the Nannochloropsis extract was compared to two well-known irritant positive control: SLS, for mature epidermis, and LA, for immature epidermis. The Nannochloropsis extract is considered as a non-inflammatory ingredient with an excellent cell viability, perfectly safe to be used on baby skin. Based on the results obtained, the Nannochloropsis extract has a good tolerance on baby skin.
Conclusion Natura-Tec Marine OceanMist CL™ offers a sustainable solution, ideally suited to the health and wellbeing of the diversity of delicate skins, helping us meet the criteria of modern life on our planet. Natura-Tec aims to respect nature at all levels of our ecosystem, with a natural, sustainable, and holistic approach of protection. We care to protect the environment by
reducing our carbon footprint with investments in earth-friendly technologies, that produces oxygen, without waste and without harming the planet. By protecting the environment, we protect human beings, improving their living conditions. Furthermore, as regards the cosmetic industry,
we care to protect our body whilst enhancing its natural beauty and respecting the uniqueness of each skin.
References 1. Nakatsuji T, Gallo RL. The role of the skin microbiome in atopic dermatitis. CME Review. 2019; vol. 122, issue 3, p.263-269
2. Nguyen MT, Hanzelmann D, Härtner T, Peschel A, Götz F. Skin-Specific Unsaturated Fatty Acids Boost the Staphylococcus aureus Innate Immune Response. Infect. Immun. 2015; Oct 26;84(1):205-15
PERSONAL CARE May 2024 PC
100 80 60 40 20 0
100 99
9 1 Untreated
Nannochloropsis extract
Figure 10: Cell viability measurement by a MTT assay 1000 234 100 Linalyl Acetate
Sodium Lauryl Sulfate
10 1 1 Untreated
Nannochloropsis extract
3. Townsend EC, Kalan LR. The dynamic balance of the skin microbiome across the lifespan. Biochemical Society Transactions. 2023; 51 (1), p71–86
4. Seité S. Skin sensitivity and skin microbiota: Is there a link? Experimental Dermatology.
Linalyl Acetate Figure 11: Inflammatory response of the immature epidermis 2018;27:1061–1064
5. Prescott et al. The skin microbiome: impact of modern environments on skin ecology, barrier integrity, and systemic immune programming. World Allergy Organization Journal. 2017; 10:29
www.personalcaremagazine.com
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Inflammatory response of tissues
RHE viability (%)
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