34 ANTI-AGEING A
■ Control ■ Placebo ■ 2% Sunflower sprout active 200
150 100 50 0 *p<0.05 versus stressed control and placebo
Figure 3: Rejuvenation study on skin explants: sunflower sprout active enhances mitochondrial function and collagen density To evaluate the response to DNA damage
To evaluate the effect of the sunflower
the levels of the DNA damage response marker γH2AX were assessed via immunofluorescence analysis. Ageing stress induced a substantial increase in DNA damage. Co-treatment with sunflower sprout extract significantly reduced this damage compared to the stressed condition, indicating that sunflower sprout extract improves the DNA repair mechanism (Figure 2B).
Rejuvenation study on skin explants Replenished NAD+
levels are recognized for their
potential to slow down and even reverse the ageing process. Consequently, the rejuvenation potential of sunflower sprouts was further investigated. To assess this, skin explants were initially
exposed to UV-A radiation to induce ageing stress and subsequently treated with either a cream containing 2% sunflower sprout active or a corresponding placebo.
A 1 0.5 0 -0.5 -1 *p<0.05 versus initial conditions and p<0.01 versus placebo Figure 4: Clinical rejuvenation study: sunflower sprout active induces a lifting effect and visibly improves signs of skin ageing PERSONAL CARE May 2024
www.personalcaremagazine.com
sprout active on mitochondrial function and proteostasis, mitochondrial protein carbonylation, a marker of mitochondrial protein damage, was analyzed by immunoblotting. UV-A radiation induced an increase in mitochondrial protein carbonylation in the skin explants. The application of sunflower sprout active
significantly reduced the mitochondrial damage compared to the stressed condition as well as the placebo, returning it to levels similar to the ones of the unstressed control (Figure 3A). The obtained rejuvenation results
demonstrate that sunflower sprout active can support mitochondrial function as well as cellular metabolism, and thus potentially slow down the ageing process of skin. Exposure to UV-A radiation plays a
significant role in photoageing due to its ability to penetrate deeply into the dermis, leading to the degradation of collagen fibres.
■ Placebo ■ 2% Sunflower sprout active * B Before After 42 days * UV-A stress + placebo 2% Sunflower sprout active UV-A stress + B Control UV-A stress
K index
Given these effects, the impact of sunflower
sprout active on collagen density was investigated within the rejuvenation model. UV-A radiation was observed to significantly reduce the collagen density in skin explants. However, the application of sunflower
sprout active led to a substantial increase in collagen density when compared to the UV-A exposed control (Figure 3B). A higher K index corresponds to an increased density of collagen fibres.
The representative images of collagen
density, obtained through XPolar® technology, illustrate the more uniform and denser distribution of collagen fibres after the use of 2% sunflower sprout active. This is evidenced by the more evenly distributed blue-green areas and a reduction in black, low-density zones, indicative of strengthened skin connective tissue.
These findings suggest that sunflower sprout active can effectively counteract
Improvement in reshaping compared to initial conditions in %
Mitochondtial protein carbonylation compared to control in %
UV-A stress
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92
