Dat a Fi l e C: \ Chem Sam
pl e Nam e: 10
===================================================================== Acq. O
per at or Acq. I nst r um M et hod : SYSTEM ent : LC1260 I nj ect i on Dat e : 9/ 11/ 2021 10: 24: 46 AM : C: \ Chem
et hod I nf o : ZO A
mAU mAU 200 200 150 150 100 100 5050 O 2 3 HN 1 O 4 N HO 1' O 2' 4' 3' OH 5' O
2" 1"
OH 6" O 5" 4" 3"
OH OH
\ . M ( Sequence M RBAX SB C18
Seq. Li ne : 14 Locat i on : 97 Inj : 1
I nj Vol um
Last changed : 9/ 10/ 2021 4: 43: 50 PM by SYSTEM M
DAD1 A, Sig=254,4 Ref=off (孔令姗\百脉根籽液20210910 2021-09-10 1641\10.D) e : 10. 000 µl NATURALS
32\ 1\ Dat a\孔令姗 百脉根籽液20210910 2021- 09- 10 1641\百脉根子成分检测 et hod)
B 79
32\ 1\ Dat a\孔令姗 百脉根籽液20210910 2021- 09- 10 1641\ 10. D \
0 0 10
===================================================================== Area Per cent Repor t
10 20 30 40
50 50
===================================================================== Sor t ed By
M
ult iplier Dilut ion Use M
Si gnal 1: DAD1 A, Si g=254,4 Ref =of f Peak Ret Ti m
Cells were stained with 0.1% Nile red (Thermo, Catalogue No. N1142) for ten minutes. Cells were analyzed by using Multimode Reader (TECAN, Switzerland) to measure the fluorescence value. Then cells were observed with the fluorescence microscopy (Leica, Germany) and taken photos for preservation.
: : :
e Type W # [m i n] [m i n] [m AU* s] LC1260 9/ 13/ 2021 3: 20: 02 PM SYSTEM
Clinical research Study design The 24-hour patch safety test was carried out in Shanghai JIABEI-test Biotech Co. Ltd with an ethical review approval (00000001). The six- hour and four-week efficacy tests were carried out at Jaka Biotech with an ethical review approval (00000004). Before the study, each participant signed an informed consent.
Si gnal 1. 0000 1. 0000
ul t i pl i er & Di l ut i on Fact or wi t h I STDs 20 30 40
Figure 1: LC composition. A: HPLC determination under 254 nm. B: Structure of 5’-o-rhamnosyluridine (T1) A
60 60
70 70
min min
i dt h Ar ea Hei ght Ar ea AU]Control
[m %
----| -------| ----| -------| ----------| ----------| --------| 1 1. 827 BB
0. 0503 16. 28426 5. 23390 0. 1105
2 2. 040 BV R 0. 1982 1957. 25269 126. 75032 13. 2824 3 2. 518 VV E 0. 1183 63. 03245 6. 92063 0. 4278 4 2. 691 VV 5 2. 891 VB 6 2. 988 BB 7 3. 476 BV 8 3. 828 VB
B
Research product The test product was a serum only contained 5% LC active ingredients. Serum base was acted as the vehicle control.
Control
Subjects A total of 30 subjects have been enrolled upon inclusion/exclusion criteria screening by the investigator. They were aged between 18 and 55 years with eight-hour oil content higher than 120 µg/cm2 on forehead.
DHT with 0.5% LC
Six-hour clinical evaluation Twenty-six oily skin subjects (20 females and 6 males) was finally included. On the day of the test, subjects used alkaline soap to clean the face, wiped the water with a dry face paper towel and sit still in the test environment for 20-30 minutes in a humidity (40-60% relative
humidity)- and temperature (20˜25°C)- controlled room. Divided the forehead and cheek area into
left and right sides according to the central position, with an interval of at least 0.5 cm between the groups, and marked it as the sample area (5% LC) and the control area (vehicle) randomly. The Sebumeter SM815 was used to measure the initial sebum on forehead. Then, a 100 µL sample was applied to
each area for a single time. Started timing after the applied sample was completely dry, and measured the sebum again after three and six
www.personalcaremagazine.com C
2000 15000 1000 5000 0
Control LA DHT with 1% LC C DHT with 0.5% LC * 0.5% LC * 1% LC
8000 6000 4000 2000 0
Control DHTR DHT with 1% LC 20µg/mL DHT Control 20µg/mL DHT
0. 1489 285. 23923 25. 81394 1. 9357 0. 0549 31. 95405 9. 11894 0. 2168 0. 0810 12. 52912 2. 31925 0. 0850 0. 2872 201. 51642 11. 45487 1. 3675 0. 2611 505. 29987 29. 02272 3. 4291 LA with 0.5% LC
0.003% LA
Control
0.003% LA
LA with 1% LC
LA with 0.5% LC
LA with 1% LC Page 1 of 2
* 0.5% LC
* 1% LC
Figure 2: Inhibitive effect of LC on SZ95 cell lipogenesis. A: SZ95 sebocytes were stimulated with the 0.003% linoleic acid (LA) treatment for 24 hours in the presence or absence of LC. Lipid droplets were stained with oil red O and Nile red to demonstrate neutral lipid (red) (scale bar = 50 µm). B: SZ95 sebocytes were stimulated with the 20µg/mL DHT treatment for 72 hours in the presence or absence of LC. Lipid droplets were stained with oil red O and Nile red to demonstrate neutral lipid (red) (scale bar = 50 µm). C: Quantitative determination of lipid synthesis after LA and DHT treatment (Nile Red staining). Data were representative of three independent experiments. (*P≤0.05 vs. LA or DHT treatment group)
March 2024 PERSONAL CARE
34.265 31.293
26.440
8.343
3.476 2.891 1.827
2.988
2.518 3.828
7.580 6.279
8.704 9.581 11.292
2.040 2.691 5.335
RFU
26.664
30.912 29.961
34.962 33.887
38.401 39.080
43.168 41.730
33.21332.607
36.977
RFU
78.446
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