78 NATURALS
Lotus corniculatus extract to inhibit lipogenesis
Lu Cheng, Jingyi Guo, Yina Lu - Jaka Biotech
In recent years, excess oil secretion on the skin surface has become a more and more common skin problem with the acceleration of the pace of life. Oily skin is frequently accompanied by large pores, and contributes to the development of acne.1
The sebaceous glands are an important
organ for synthesizing lipids and an important source of sebum for oily skin.2,3 Sebum can maintain the integrity of the cutaneous lipid barrier, transport antioxidants to the skin surface, and have antimicrobial, anti- inflammatory activity.4-8
Although their function
is important, excess oil secretion on the skin surface cause great discomfort and should be treated.9 Sebocytes are the main cells of sebaceous gland structure. Excessive sebum secretion on the skin surface is closely related to the differentiation, synthesis and secretion of lipid by sebocytes. Human sebocytes are specialized epithelial
cells that synthesize and secrete lipid through different ligand-receptor signaling pathways such as peroxisome proliferator-activated receptor (PPAR), androgen receptor (AR), liver X receptor (LXR), melanocortin receptor (MCR) and insulin-like growth factor (IGF)-1 receptor.10 Linoleic acid (LA) as an activator of
PPARγ results in the generation of lipids through sebocytes differentiation.11-13 Dihydrotestosterone (DHT) which converted intracellularly from testosterone by 5α-reductase enzyme exerts effects inducing cell proliferation, differentiation and regulating lipid synthesis by binding to the androgen receptor present on the sebocytes.14-16
Patients
with acne have higher testosterone levels and higher DHT production rates on skin.17 In this study, we focus on the botanical
intergradient for oily skin treatment, especially for large pores improvement.18,19
Lotus
corniculatus extracts and its active components have therapeutic properties, such as anti- inflammatory effects,20-22 activities,23
anti-proliferative and antioxidant effects.24 However,
there is a lack of research on its efficacy against sebaceous gland and the active ingredient works as the inhibitor of lipogenesis. We used a special extraction process to
obtain the extract of Lotus corniculatus seed (LC), and identify the active components by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) to study its effects on lipogenesis and sebum secretion in vitro and in vivo.
PERSONAL CARE March 2024
Materials and methods LC composition analysis The extract of Lotus corniculatus seeds (Smopore LC) was obtained from our laboratory. Seeds were extracted with ten times weight of water at 80-85°C for two hours. The extract was adsorbed by D101 macroporous adsorption resin, and eluted with water and 95% ethanol in turn. The 95% ethanol eluent was used to
recover the solvent to obtain the LC. The active composition by high performance liquid chromatography (HPLC) on ZORBAX SB C-18 under 254 nm. Nuclear magnetic resonance (NMR) was used to identify the T1 and T2 compound.
Cell culture The immortalized human SZ95 sebaceous gland cell line25
was cultured in DMEM/F-12 (1:1) medium
supplemented with glutamax, 1% P/S, 9 ng/ml human EGF, 3 ng/ml human KGF, 10% fetal bovine serum (FBS). Passage every three days.
Cell staining SZ95 sebocytes were seeded in 96-well plates (Corning, USA) at 0.8×104cells/well. After overnight, medium was changed to 0.003% LA (TCL, Catalogue No. L-0078) with or without LC solution, and then the plates were incubated for another 24 hours at 37°C. 20µg/mL DHT (Selleck, Catalogue No. S4757) was added to
ABSTRACT
The increasing popularity of botanical ingredients in skincare and haircare is attributed to their efficacy and natural origins. However, limited research has focused on the synthesis and secretion of sebum. This study aims to explore the novel composition of Lotus corniculatus seed extract (Smopore LC) and its inhibitory effects on lipogenesis in SZ95 sebocytes. Topical application of Smopore LC resulted in an immediate and long-lasting reduction in sebum content and sebum area on Chinese oily skin, achieved by downregulating sebaceous lipogenesis through the LA and DHT pathway. Smopore LC shows promise as a new botanical ingredient for oily skin.
cells with or without LC solution for 72 hours. Oil Red O solution was prepared by mixing
0.5% Oil Red O (Sigma, Germany) in 99% isopropanol and dH2
O and then filtered.26 Cells
were rinsed with PBS and stained with 0.5% Oil red O for 15 minutes, and then washed in 40% isopropanol and dH2
O. Lipid droplets in cells
were observed under an inverted microscope (Leica, Germany) and taken photos for analysis and preservation.
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