70 HYGIENE
None Poly I:C CTRL Ferm. Agent
200 150 100 50 0 0 1 mg/ml Figure 1: Modulatory action on IL-6, IL-8 and CCL2/MCP-1
Introducing postbiotics However, the use of living bacteria on scalp or skin poses several challenges, such as skin safety, the compatibility with the preservatives and the ability of the microorganisms to grow and inhabit the host. The innovative approach we propose is represented by the use of postbiotics. The term refers to molecules - not living, nor inactivated microorganisms - but released by beneficial bacteria that mediate the beneficial effects of probiotics itself. Postbiotics include bioactive peptides enzymes, short-chain fatty acids (SCFAs), biosurfactants, vitamins and more. It has been hypothesised that these substances are responsible for probiotic efficacy. Compared to probiotics, postbiotics can interact in a more targeted and direct way with local microbiota and host cells. Recent research highlighted the main properties of postbiotics including antioxidant, antimicrobial and immunomodulatory activities.12 Postbiotics can therefore represent a novel
frontier in hair care to prevent ageing of hair, hair growth disorders, e.g. alopecia, and scalp issues such as dandruff, by directly counteracting oxidative stress and inflammation in HFs, due to their high specifity and selectivity of action on resident microbiota and host cells. An in vivo study showed the efficacy of
a cosmetic gel containing postbiotics for the treatment of alopecia areata. A significant improvement in hair regrowth was observed in the subjects treated with the active product in comparison to placebo.13 We here demonstrate the ability of Kalibiome
postbiotics - a pull of postbiotic molecules obtained from L. paracasei through an original and unique biofermentation - in reducing the expression of inflammatory mediators, decreasing oxidative stress by reducing the production of free radical species, and inhibiting
S.aureus biofilm formation, thus suppressing the main causes involved in scalp disorders previously discussed.
PERSONAL CARE October 2022
Kalibiome postbiotics Solutions aimed to preserve the skin and hair scalp microbiota balance have become one of the main cosmetic industry priorities. In the past years, living probiotics, tyndallised bacteria, lysates or prebiotics use has expanded. However, the production of stable, reproducible and safe formulations containing most of these ingredients remains a topic of discussion. In light of the previous scientific literature
background, it can be asserted that bacteria interact with each other and skin and hair scalp cells, using complex network of molecules, the postbiotics, which represent a new class of bacteria-derived factors, helpful in regularising the microbiota balance, reinforcing skin, hair and scalp defense and other beneficial effects. Through a patented biotech fermentation
process (PB Tech), Kalibiome postbiotics for cosmetic applications were designed and produced starting from L. Paracasei, a species known for its beneficial skin effects. Kalibiome postbiotics are produced naturally
and released by living microbes and extracted by a technique evading bacterial fragments or toxins presence enabling to obtain highly reproducible output, avoid interferences with cosmetics preservatives and formula stability issues, while optimising the safety profile and increase the efficiency of the microbiota regulation process on skin and hair. Kalibiome postbiotics are pure substances
that do not contain any residue of bacterial fragments nor culture broth media residues or bacteria toxins. Due to their reproducible composition, organoleptic features, long shelf life, safety, handling and efficacy, the use of postbiotics is an elegant and efficient approach to directly provide the skin with microbial actives. In addition, Kalibiome postbiotics come
in hydro-soluble powdery form, they have a preservative-free composition and a significant applicative versatility, as they can be included
in several cosmetics forms while avoiding interferences with cosmetics preservatives and formula stability issues.
Kalibiome: biological targets to counteract hair disorders and stimulate hair follicle Kalibiome postbiotics were developed with the aim of obtaining specific metabolites able to modulate the expression of: ■ Interleukin 6 (IL-6), cytokine acting as mediator of the inflammatory process leading to the miniaturisation of the hair follicle and shortening of the anagen phase ■ Interleukin 8 (IL-8) and Monocyte chemoattractant protein-1 (MCP-1), chemokines enhancing the inflammatory response on the hair bulb subject to UV rays and exposed to increased risk of catagen phase onset acceleration and hair greying ■ Thymic Stromal Lymphopoietin (TSLP), cytokine demonstrated to be significantly increased during the anagen phase of the hair cycle and able to promote hair growth in response to injury.14 TSLP protein is found in two variants: Long-form TSLP (linked to type 2 immune responses, highly induced in pathological conditions such as allergic diseases) and Short-form TSLP (sfTSLP) constitutively expressed in healthy human epidermis. sfTSLP was found to be upregulated throughout the hair cycle, reaching peak expression during mid-anagen, when hair follicles extend deeper into the tissue, and then downregulated to lowest expression during catagen. Scientific evidence suggests that TSLP functions locally in the hair follicle microenvironments to promote tissue regeneration Furthermore, Kalibiome proved to act at additional functional values, by: ■ Reducing free radicals induced by UV radiations: the oxidative stress is among the most relevant factors of risk triggering the onset
www.personalcaremagazine.com 10 100
IL-6 200 150 100 50 0 0 1 mg/ml 10 100
IL-8 200 150 100 50 0 0 1 mg/ml 10 100
CCL2/MCP-1
% of response (normalized to poly I:C)
% of response (normalized to poly I:C)
% of response (normalized to poly I:C)
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