30 SKIN BARRIER


Methods Preparation of the Ca2+


Stratum corneum Stratum lucidum


Basement membrane


Stratum granulosum Stratum spinosum Stratum basale


system To prepare the novel Ca2+


double cone carrier delivery system,


glycerol, pentylene glycol and ddH2 O were


mixed at 60°C, soy lecithin was added, then the dispersion pumped repeatedly through a high- pressure homogeniser (M700, MicrofluidicsTM, USA) at 1200 bar. Then, a CaCl2


the liposomal dispersion, forming a Ca2


solution is slowly added to +-


phospholipid double cone complex, which is again homogenized at high-pressure. The final structure of the Ca2


confirmed by freeze-fracture transmission electron microscopy (FFTEM).


Ca2 Figure 1: The epidermal calcium gradient A + conc.


RHE culture and treatment Using human keratinocyte progenitor cells, 3D reconstructed human epidermis (RHE) models were established for the experiment. During the differentiation process, the RHE were either treated with 1.1 mM calcium basal (control), 0.3 mM calcium basal (reduced calcium), 0.3 mM calcium basal and 1.1 mM CaCl2 mM calcium basal and 0.1 % Ca2 apical.


apical, or 0.3 + vector system The 3D models were harvested at day


9, fixed and the morphology analysed by hematoxylin and eosin (H&E) staining, whilst the expression of loricrin (antibody No.905104, BioLegend, USA) was determined by immunohistochemistry analysis.


B


Skin explant treatment Human full skin explants, obtained from donor tissues of elective cosmetic surgery, were treated topically with 20 mg of a gel containing either 2 % Ca2


+ vector system, 0.884 mM CaCl2


embedded in paraffin. Then 5 µm tissue sections were prepared


Figure 2: Structure and EM image of the Ca2+ vector system


and mounted on glass slides prior to deparaffinization. Slides were then stained with H&E for tissue structure analysis. Protein expression of involucrin (antibody


,


or a placebo gel, for 24 hours using Franz diffusion cells. After exposure, the explants were washed with ddH2


O, fixed in formaldehyde, and + vector system was


PERSONAL CARE October 2022


www.personalcaremagazine.com


Epidermis


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100  |  Page 101  |  Page 102  |  Page 103  |  Page 104  |  Page 105  |  Page 106  |  Page 107  |  Page 108