NATURALS


incubated our samples for one more hour. Cell morphology was assessed by microscope to quantify degranulation and Cromolyn sodium at 1mg/Ml was used as a positive control. A microscopic image from mast cells


showing the morphological difference between intact and degranulated cells is shown in Figure 4. Figure 5 compares the degranulation rate


for the positive control sample and samples treated with vitamin B12. This shows that at a concentration of 0.00015%, vitamin B12 inhibited mast cell degranulation by a significant 27%. Our findings indicate that by stabilizing


Figure 4: Representative microscopic image from untreated mast cells showing morphological difference between intact and degranulated cells


free radicals leading to the release of mediators such as histamine, proteases, and cytokines - a process known as degranulation. When mast cells in the skin degranulate,


they cause allergic reactions and symptoms such as itching, stinging, burning, redness, and dry, sensitive skin. If we can find a way to inhibit mast cell degranulation, we can counter these uncomfortable and unpleasant skin conditions, and improve skin appearance. To this end, our second study investigated


the ability of vitamin B12 to block this process. Figure 3 illustrates the mechanism of action for mast cell degranulation and where Vitamin B12 could intervene to inhibit it.


Methodology and findings The aim of this study was to investigate the inhibition effect of vitamin B12 on the degranulation of pre-activated mast cells in vitro. To do this, we incubated mast cells in six well plates for 24 hours and then added vitamin B12 at a concentration of 0.00015%. After another hour, we added C48/80 - a


polymer that promotes histamine release and mast cell degranulation at 50ul/mL - and then


100 80 *** 60 40 20 0 Stimulated cell Positive control Vitamin B12 *** p-value<0.001


Figure 5: Degranulation of mast cell with vitamin B12 and positive control. The y-axis scale corresponds to the percentage of degranulated cells


www.personalcaremagazine.com October 2022 PERSONAL CARE ***


mast cells and helping to block the release of histamine, vitamin B12 can help protect against inflammation induced skin barrier damage and relieve skin sensitivity and irritations cause by environmental stress factors.


Study 3: Could vitamin B12 play a role in preventing over colonisation of skin microbiota associated with skin inflammation? Imbalances in skin microbiota composition have been associated with several non-pathological skin conditions, including skin sensitivity and irritation. This has generated a growing interest within the personal care industry in approaches that foster the natural, individual balance of the skin microbiome. For our third study, we analysed the impact


of vitamin B12 on the colonization of one of the most undesirable, pathogenic bacteria in the skin microbiome: S. aureus. When there is an imbalance in the skin microbiome, S. aureus becomes dominant and affects skin barrier function via bacterial proteases that eventually trigger inflammation processes.


Methodology and findings For our analysis, we added vitamin B12 at three concentrations to a medium culture which


-43% -27.2%


45


Degranulation rate (%)


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