HYGIENE SymGuard® CD ■
100 80 60 40 20 0
Control 0.075% 0.15% 0.3%
Figure 2: Relative biofilm activity in percentage with different concentrations of SymGuard CD
With a commonly recommended oral care
use level of up to 0.3% or higher, the cosmetic hygiene ingredient is a viable solution for oral hygiene as part of cosmetic products. This needs to be evaluated case by case depending on a suitable masking taste profile.
Oral biofilm model (ex vivo microbiome) While the S. mutans biofilm studies represent a single species result, Symrise has developed a representative oral biofilm model which is closer to real life and thus can explore effects on complex natural microbial communities. Dental plaque biofilm organisms are grown on
hydroxyapatite (a mineral found in tooth enamel) and model oral biofilms on tooth surfaces. The influence of the cosmetic hygiene ingredient has been evaluated using this oral biofilm representing a natural oral microbiome. The biofilm activity was determined via
fluorescence. Three independent experiments were performed starting with saliva collection as a source for the microorganisms. Each experiment included four treatments of two minutes with different concentrations (ranging from 0.0% to 0.3%) of the cosmetic hygiene ingredient in a saline buffer. The biofilm activity was determined 18 hours
after the last treatment. As control, a pure saline buffer was used, and the biofilm activity of the control condition was defined as 100% relative biofilm activity. As a result, the activity of the oral biofilm
Mouthwash with 0.3% SymGuard CD ■ Placebo ■
4.00 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00
CH3 * * D0 D7 D28
Figure 3: Sensory analysis of breath of the volunteers assessed by an expert (*significant difference to baseline, p<0.05)
www.personalcaremagazine.com
600 500 400 300 200 100 0
SH level
79
TABLE 2: MOUTHWASH FORMULATIONS INCI
Water Propylene Glycol Hydroxyacetophenone (SymSave®)
1,2-Hexanediol, Caprylyl Glycol (SymDiol®) Glycerin
PEG-40 Hydrogenated Castor Oil Flavor
Phenylpropanol, o-Cymen-5-ol, Decylene Glycol (SymGuard CD) Sodium Saccharin
decreased with increasing concentrations of the cosmetic hygiene ingredient (Figure 2). There is already a clear effect visible with a concentration of 0.15% cosmetic hygiene ingredient and a concentration of 0.3% cosmetic hygiene ingredient reduces the activity of biofilm by almost half.
Oral odour evaluation (in vivo) The objective of this study was to evaluate the oral odour efficacy of a mouthwash containing 0.3% the cosmetic hygiene ingredient in
Placebo 92.05 2.00 0.30 0.50 4.00 0.80 0.30 -
0.05
% w/w Material
0.3% SymGuard CD 90.55 2.00 0.30 0.50 4.00 2.00 0.30 0.30 0.05
comparison to placebo (Table 2). For this evaluation, 35 healthy subjects used the mouthwash twice a day (morning and evening) for 28 days after a two-week pre- conditioning phase, in which they were only allowed to use a standard toothpaste. The oral odour was evaluated through
sensory assessment by trained experts rating (score 0 (no odour) to 5 (severe odour)) and via analytical methods using a sulfide detector (Halimeter) on the first day of the test and after seven and 28 days of use.
Mouthwash with 0.3% SymGuard CD ■ Placebo ■ CH3
SCH3 D0 D7 D28
600 500 400 300 200 100 0
level
* * D0 D7 D28
Figure 4: Average concentration of different organosulfur compounds analyzed from the breath of volunteers (*significant difference to baseline, p<0.05)
March 2023 PERSONAL CARE
Sensory analysis of bad breath
Relative Biofilm Activity in %
ppb
ppb
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