68 SKIN CARE


Skin Hydration (%) 58


56 54 52.99 52 50 48 Before use After 1 week


Figure 4: Skin hydration changes after one week of application. Skin hydration was measured using a Corneometer® before use and after one week of application. The test product group showed an increase in skin hydration, while the control group exhibited a slight decrease over the same period. *p < 0.05 vs before use, #p < 0.05 Test product vs control


A


160 140 120 100 80 60 40 20 0


135.29 *


117.04 *


100


106.45 *


147.09 *


52.21 51.23 #


Fucose Glucose Xylose


Mannose


Glucuronic acid


TABLE 1: MONOSACCHARIDE COMPOSITION OF THE TREMELLA MYCELIAL POLYSACCHARIDE


Control ■ Test product ■ 56.54 Sugar Content (g)


Tremella fuciformis– derived mycelial polysaccharide extract (TFMPs)


17.1 1.3


21.0 40.3


17.3


Conventional Tremella fruiting body extracts


11.2


50.5 15.2 8.5


13.0


than the control group (p < 0.05), confirming a meaningful moisturizing effect at the human skin level. Importantly, no adverse skin reactions such as erythema, itching, or irritation were observed throughout the study period, indicating excellent skin compatibility.


Additional skin benefits: wrinkle- related markers and antioxidant Beyond hydration, in human dermal fibroblasts (NHDF cells), treatment enhanced pro-collagen type I synthesis, a key marker associated with wrinkle improvement and dermal matrix support. These results suggest that sustained hydration provided by the polysaccharide network may be complemented by structural skin benefits, reinforcing its role as a multifunctional cosmetic ingredient. Furthermore, the Tremella mycelial


polysaccharides demonstrated intracellular reactive oxygen species (ROS) reduction in H2


O2 -stimulated


keratinocytes, indicating a protective antioxidant effect at the cellular level. This reduction in oxidative stress is relevant to maintaining skin barrier integrity and preventing moisture loss associated with environmental damage.


Control 0.1% 0.5% TFMPs (%) B 1% 10 Asc.A. (ug/mL)


Discussion High-molecular-weight HA has long been utilized as a moisturizing ingredient in cosmetic formulations, primarily due to its exceptional water-binding capacity. However, its hydration mechanism is largely based on the formation of a continuous surface film, which can limit flexibility, penetration, and sensory comfort. The present study demonstrates that mannose- enriched Tremella mycelial polysaccharides provide an alternative hydration strategy characterized by both structural and biological activity. From a molecular perspective, the Tremella-


derived polysaccharide evaluated in this study exhibits a branched glucuronoxylomannan architecture with a molecular weight comparable to that of 1M Da HA. Unlike linear HA chains, the highly branched


Figure 5: Enhancement of Procollagen Type I synthesis in dermal fibroblasts. The polysaccharide significantly stimulated procollagen synthesis compared with the control, suggesting wrinkle-improving potential. Data are mean ± SD of triplicate experiments. *p < 0.05 vs control (A) Procollagen production, (B) Intracellular collagen fibre formation


PERSONAL CARE MAGAZINE May 2026


structure is likely to promote the formation of a three-dimensional hydrated network rather than a rigid planar film. This structural distinction may explain the observed balance between durable moisture retention and flexible hydration behaviour, as suggested by hydration performance and molecular characteristics. At the cellular level, the polysaccharide


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