38 LONGEVITY
conceptual parallel for topical use of Vicia faba seed–derived fractions aimed at supporting skin resilience against everyday excessive screen time. Studies with SC Complex were performed on
consumer groups of all ages, all of whom with elevated daily screen time. Anti-ageing parameters starting from the eyes and radiating across the entire face are brought into real life and the present moment under the light of digital longevity. SC Complex showed to reduce skin ageing features of people with elevated screen time to a level of people who have low daily screen time.
Efficacy: in vitro studies for dermal integrity Increase of essential dermal compounds The basis for stable, structurally defined and youthful-looking skin is an organized network of essential proteins in the dermis. In in vitro studies, SC Complex was shown to optimally stimulate collagen I and III, elastin and LOX in the skin in order to counteract structural loss caused by stress-related influences.
Method Fibroblasts of a 39 year-old female volunteer were seeded into 24-well plates and incubated in a CO2
incubator (37°C, 5% CO2 ) overnight. When
the cell laying rate in the 24-well plate reached 50–60%, test substances were added to the wells in different concentrations (control (0%), 0.05% and 0.5%, for each concentration: N = 3). Then, the 24-well plate was further incubated ), for 24 hours. Subsequently, the
(37°C, 5% CO2
fibroblasts were fixed with 4% paraformaldehyde. After 30 minutes of fixation, immunofluorescence detection was performed, pictures were taken under a microscope, and the collected images were analysed. GraphPad Prism software was used to
analyse the data, and the result was expressed as Mean ± SD. The comparisons between groups were performed using t-test statistical analysis. Statistical analysis was two-tailed. p <0.05 was considered as significant difference and p <0.01 was considered as extremely significant difference. The integrated optical density (IOD)/average
cell count reflects the content of the different compounds of interest and is presented in the diagrams below. Antibodies: collagen I antibody
MMP-1 1
0 Control
SC Complex 0.05%
SC Complex 0.5%
Figure 3: Treatment with SC Complex significantly increases elastin
(CST), collagen III antibody (Wuhan Sanying), Elastin antibody (Abcam), LOX antibody (Wuhan Sanying).
Results For all four dermal compounds of interest, it could be shown that the treatment with different concentrations of SC Complex leads to an elevation of their expression. These results indicate that the treatment of
skin with SC Complex can compensate for the increased breakdown of collagen I, collagen III, elastin and LOX, which is a common phenomenon for people with a high screen time (Figures 1, 2, 3 and 4).
*
MMP-3 1
** 0 Control
SC Complex 0.05%
SC Complex 0.5%
Figure 4: Treatment with SC Complex significantly increases LOX
Reduction of breakdown of essential dermal compounds In further experiments, the gene expression of MMP-1, MMP-3 and MMP-9 was investigated. The elevated expression of these genes is strongly associated with the increased breakdown of the dermal components which give skin its firmness and elasticity.6 MMP-1 can cleave collagen I and collagen III.
MMP-3 has a broader action spectrum, including proteoglycans, fibronectin, laminin and nonfibrillar collagens, such as collagen III. MMP-9 breaks down type IV and V collagens in the basement membrane as well as elastin and various glycoproteins and proteoglycans.
MMP-9 1
Collagen I 2
** ** 1 1 **
Collagen III 2
**
0 Control
SC Complex 0.05%
SC Complex 0.5%
Figure 1: Treatment with SC Complex significantly increases collagen I
Elastin 2
** * 1 1 **
0 Control
SC Complex 0.05%
SC Complex 0.5%
Figure 2: Treatment with SC Complex significantly increases collagen III
LOX 2
**
*
0 Control SC Complex (0.1%)
Figure 5: Treatment with SC Complex significantly reduces the expression of MMP-1
PERSONAL CARE MAGAZINE May 2026
0 Control SC Complex (0.1%)
Figure 6: Treatment with SC Complex significantly reduces the expression of MMP-3
0 Control SC Complex (0.1%)
Figure 7: Treatment with SC Complex significantly reduces the expression of MMP-9
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Mean
Mean
Relative IOD/cell count average
Relative IOD/cell count average
Mean
Relative IOD/cell count average
Relative IOD/cell count average
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