66 SKIN MICROBIOME
keratinization of the stratum corneum and ultimately accelerated ageing.17,18 Also, the same for hair. Dryness reduces shine and combability and makes hair more susceptible to damage. And in recent years it has been discovered that scalp care and keeping the scalp moisturised is essential as it determines the health and condition of the hair and prevents scalp and hair problems.19 The requirement for moisturisers is that
they should work as long as possible, without interruption, so that the skin and hair are always moisturised.20
Aminoflora is proven to deliver
long-lasting moisturisation with an excellent feeling of use.
Skin care efficacy The following test was conducted to verify the long-lasting moisturising effect on the skin with Aminoflora. In this experiment, a solution consisting of glycerin and arginine, in the same molar concentration as Aminoflora, was used as a comparative reference. Five subjects aged 20 to 30 had their inner
forearms cleansed, and the water content of the stratum corneum was measured before treatment with the sample. Subsequently, a 1% Aminoflora aqueous solution or a glycerin- arginine mixture (control) was applied as the test solution and left for 15 minutes. The test area was cleansed with a foaming
cleanser for 30 seconds, followed by a one-minute rinse with running water. Water content in the stratum corneum were measured immediately after cleansing, and at 15, 30, 45, and 60 minutes later. As shown in Figure 6, the Aminoflora aqueous solution showed higher stratum corneum water content than the control immediately after cleansing. This suggests that
FIGURE 10: FORMULATIONS OF SHAMPOO AND CONDITIONER
Ingredients/ INCI SHAMPOO
Aminoflora (Dihydroxypropyl Arginine HCI, Water)
Promois EFLS-F*
Sodium laureth Sulfate (25%), Water PEG-120 Methyl Glucose Dioleate Citric Acid, Sodium Citrate Polyquaternium-10 Methylparaben Water
CONDITIONER Steartrimonium Chloride (28%), Water
Behentrimonium Chloride (80%), Isopropyl Alcohol (20%)
Glyceryl Stearate Cetyl Alcohol Paraffin
Octyldodecanol
Isopropyl Myristate Propylparaben Methylparaben Dimethicone
Dimethicone (500cs) Water
* Sodium Lauroyl Hydrolyzed Silk, Water (SEIWA KASEI)
4.5 1.2 1.2
4.8 1.8
0.6 1.2 0.1
0.1
3.0 1.0
up to 100 % w/w 1.0
37.5 8.0 1.0
0.5 0.3 0.2
up to 100 Affinity Preference
20 15 10 5 0
Control ■ Aminoflora■
Non-friction
Smoothness Non-stickiness Figure 8: Sensory evaluation of skin toners
Aminoflora has high resistance to cleansing. Additionally, over time, Aminoflora
maintained higher stratum corneum water content, indicating its persistent presence on the skin. Thus, Aminoflora was confirmed to have a long-lasting moisturising effect, meaning that Aminoflora, by effectively absorbing into the skin, enhances resistance to cleansing, ensuring a long-lasting moisturising effect that persists on the skin even after the most drying cleansing. A long-lasting moisturising effect of
Aminoflora has been proven, yet the feeling of use in the moisturiser is often mentioned. Sensation of stickiness, a common worry associated with moisturisers. Therefore, we conducted a sensory evaluation test on the feeling of Aminoflora. In the test, two skin toner formulations were
prepared, 11 subjects used a toner containing 8% glycerin and 1% Aminoflora, and a Control toner containing 8% glycerin without Aminoflora (Figure 7). Each evaluation criterion was scored as two points for high, one point for equal, and zero points for low, and a total score was calculated. The results showed that the toner containing
Aminoflora was non-sticky compared to the control. Additionally, it demonstrated a favourable sensory texture in other aspects (Figure 8). The two formulations used in this test contained a high amount of glycerin. This suggests that Aminoflora functions as a
moisturiser with a non-sticky feeling, and when used in conjunction with other moisturisers, it suppresses the sticky feeling associated with other moisturisers.
Hair care efficacy The following test was conducted to verify the long-lasting moisturising effect of Aminoflora
PERSONAL CARE June 2024
16.0 14.0 12.0 10.0 8.0 6.0 4.0 2.0 0
Glycerin n=3, ** p<0.01 (t test) **
Moist feeling
Aminoflora
Figure 9: Measurement of adsorption amount to hair
on hair. Bleached hair was immersed in either a 1% Aminoflora aqueous solution or a 0.3% glycerin aqueous solution, shaken at 40°C for ten minutes. After washing with deionized water,
the active component of Aminoflora, Dihydroxypropyl Arginine HCl, or glycerin was extracted from the hair, and quantification was measured using LC/MS (LCMS-2020 (Shimadzu)). The results confirmed that while glycerin was
hardly retained, Aminoflora remained on the hair at over 100 times the level of glycerin (Figure 9). This demonstrates that Aminoflora persists on the hair even after washing, confirming its long- lasting moisturising effect to the hair.
Scalp care efficacy The following test was conducted to verify the scalp care effect of Aminoflora. A shampoo and a common conditioner
formulation were prepared, with the shampoo
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Adsorpition amount to hiar (mg/g)
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