SKIN CARE T0 T56
35
Figure 3: Improvement of the skin’s texture treated with 2% active ingredient (resurrection plant and green coffee extract) at baseline (T0) and after 56 days (T56)
Analysis of spots on back of hands Analysis of hand skin involved acquiring images with a Digital Canon Camera EOS 60 D (Canon) and using a Chroma Meter CR 400 (Konica Minolta Sensing Americas, Inc.) for measuring spots lightening and skin homogeneity. Notable improvements in various skin features on both hands and face were observed after 28 and 56 days of consistent use. The results showcased notable
improvements in various skin features on both hands and face after 28 and 56 days of consistent use.
Results The active ingredient at 2% demonstrated a substantial reduction of 25.9% and 30.8% in hand spot length after 28 and 56 days, respectively, while the placebo formulation yielded no discernible effects. A time-dependent enhancement of
44.7% and 63.2% in hand skin homogeneity was observed at day 28 and day 56 (Figure 2), respectively, with the active ingredient, whereas the placebo exhibited an increase in skin homogeneity solely after 28 days, with no discernible effects at day 56. These findings highlight the efficacy of the resurrection plant and green coffee active ingredient in promoting skin brightness and pro-ageing effects.
Analysis of spots on face Facial analysis involved acquiring three images (front, left, and right sides) of volunteers using Visia CT equipment (Canfield Scientific, Inc.). These images were standardized using a digital Canon Camera EOS 60D for subsequent analysis of spot lightening through the FrameScan® software (Teradyne). Skin evenness and spot lightening on the
facial and hand areas were determined using the total surface of spots and the Haralick homogeneity index. Clinical dermatological evaluations were conducted by a dermatologist on days 0, 28 and 56.
www.personalcaremagazine.com
Results The active 2% formulation significantly increased facial skin homogeneity, improving by 44% and 21.5% at day 28 and 56 respectively. In contrast, the placebo showed a 14.3% improvement in skin homogeneity after 28 days of treatment, with no effects observed at Day 56. Facial images of volunteers further illustrated the overall improvement of skin features when compared to the placebo group.
Analysis of skin anisotropy (skin texture and elasticity) Furthermore, the evaluation extended to the anisotropy index on volunteers’ forearms after 56 days of treatment with either placebo or the resurrection plant and green coffee active at 2%. Measurements at days 28 and 56 were
compared with baseline measurements at day 0 before treatment initiation. The images were acquired using Visioscan® equipment (Courage & Khazaka electronic GmbH) to determine skin roughness and anisotropy. The findings revealed a significant 8.3%
reduction in skin anisotropy with the active treatment after 56 days, whereas the placebo led to a notable 9.7% increase in skin anisotropy after only 28 days of treatment. The best result obtained among the subjects
was a 50.2% reduction in the skin anisotropy index after 56 days of treatment with active ingredient 2% formulation (Figure 3).
Results The anisotropy index was also evaluated on the forearm of volunteers after treatment with placebo or MflCas 2% by 56 days. The measurements were performed at days 28 and 56 after treatments and compared with measurements of day 0 before treatment. The results demonstrated MflCas 2%
significantly reduced the skin anisotropy by 8.3% after 56 days of treatment, whereas placebo significantly increased in 9.7% the skin anisotropy after 28 days of treatment.
Conclusion The result of in vitro study showed the action of the resurrection plant and green coffee active in reducing lipofuscin induced by ultraviolet radiation in human fibroblasts, acting as a detoxifying and protective agent. As a result of these differential mechanisms,
the clinical study underscores the potential of the resurrection plant and green coffee active formulation in delivering remarkable skin brightening and pro-ageing effects, making it a promising candidate for advanced skincare formulations.
PC
References 1. Choi W et al. Molecular and histological characterization of age spots. Experimental Dermatology. 2017; 26, n. 3, p. 242-248
2. Porta EA. Pigments in aging: an overview. Annals of the New York Academy of Sciences. 2002; 959, n. 1, p. 57-65
3. Bourne GH. Lipofuscin. In: Progress in Brain Research. Elsevier. 1973; p. 187-201
4. Kim HS et al. Autophagy in human skin fibroblasts: impact of age. International Journal of Molecular Sciences. 2018; 19, n. 8, p. 2254
5. Keller JN et al. Autophagy, proteasomes, lipofuscin, and oxidative stress in the aging brain. International Journal of Biochemistry & Cell Biology. 2004; 36, n. 12, p. 2376-2391
6. Nam T et al. Emerging paradigm of crosstalk between autophagy and the ubiquitin- proteasome system. Molecules & Cells. 2017; 40, n. 12, p. 897
7. Moore JP et al. An overview of the biology of the desiccation-tolerant resurrection plant Myrothamnus flabellifolia. Annals of Botany. 2007; 99, n. 2, p. 211-217
8. Affonso RCL et al. Phytochemical composition, antioxidant activity, and the effect of the aqueous extract of coffee (Coffea arabica L.) bean residual press cake on the skin wound healing. Oxidative Medicine & Cellular Longevity. 2016
June 2024 PERSONAL CARE
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80
