SKIN MICROBIOME Control Amitose R-PD Aminoflora Glycerin Glucose
65
Figure 5: Measurement of M. furfur increase
Staphylococcus aureus (S. aureus) on the skin can cause skin microbiome imbalance.12
Therefore, the excessive proliferation
of S. aureus will roughen the skin and cause inflammation. Utilization experiments were prepared as
below. S. aureus (NBRC13276) transferred to SCD agar medium and incubated at 30°C. 4 mL of the following BPW (Pepton 10.0 g/L, NaCl 5.0 g/L, Na2
HPO4 9 g/L, KH2 PO4 1.5 g/L) medium
was mixed with 0.5% BPW medium containing 10% carbon source and 0.5 mL of each bacterial solution (1-9×107
cfu/mL) and incubated at
37°C. Optical density (OD620) was measured immediately after inoculation, 24 hours, and 48 hours later. The results indicate that Aminoflora cannot
be utilized by S. aureus, but sodium PCA and hyaluronic acid significantly promote the growth of S. aureus (Figure 4).
Utilization of bad microorganisms (for scalp) Recent studies have revealed a link between healthy scalp and microbiome. According to the research, Malassezia furfur
(M. furfur), bacteria that cause seborrheic dermatitis and dandruff.14,15
The scalp is rich in
30 25 20 15 10 5 0
-5 application Before
application and washing immediately
After Among
them, the detection rate of S. aureus in the skin lesions of atopic dermatitis (AD) patients is the highest.13
FIGURE 7: FORMULATIONS OF SKIN TONER Skin toner
Control
Aminoflora (Dihydroxypropyl Arginine HCI, Water)
Glycerin
Butylene Glycol Alcohol
Citric acid Sodium Citrate*
Phenoxyethanol Water
* Adjusted to pH 5.5
sebaceous glands that produce and release sebum to the skin surface. M. furfur benefit from the lipids of the sebum. They use sebum as a nutrient source and proliferate in proportion to the increase in sebum secretion.16 We prepared utilization experiments to test
whether Aminoflora and other moisturisers (glucose and glycerin) promote the proliferation of M. furfur. We cultured M. furfur in LNA medium at
30°C for two days. Prepare a bacterial solution (1.8 x 108
cfu/mL) by suspending cultured
M. furfur in low-nutrient LNA medium. Mix 1 mL of sample aqueous solution with 9 mL of
Control ■ Aminoflora■ Non application■ n=5, * p<0.05 compared with non application
High -
8.0 4.0 4.0
q.s. 0.5
low-nutrient LNA agar medium to prepare agar medium.
Aminoflora 1.0
8.0 4.0 4.0
q.s. 0.5
up to 100 up to 100
Summary As a result, it was confirmed that Aminoflora and Sodium PCA promote the growth of good microorganisms, S. epidermidis. Sodium PCA and hyaluronic acid promote the growth of bad microorganisms, S. aureus. Glycerin and sorbitol promote the
overgrowth of opportunistic microorganisms, C. acnes, and for scalp, glucose and glycerin promote the growth of bad microorganisms, M. furfur. In other words, according to our
experiments, it has been proved that every moisturiser promotes the reproduction of various microorganisms to a greater or lesser extent, affecting the balance of the skin microbiome.
When the skin is in a healthy state, such
microorganisms change would quickly return to their original state, but when the skin is in a rough state or beginning state of roughness, the skin microbiome would become even more disrupted, which would aggravate the skin condition. Whereas Aminoflora was confirmed to be
* * * Low 15 min 30 min 45 min 60 min
effective in increasing good microorganisms selectively without affecting growth promotion of the bad microorganisms, although its long-term presence on the skin increases the possibility of affecting skin microbiome.
Figure 6: Measurement of water content in the epidermal stratum corneum
www.personalcaremagazine.com
Long-lasting moisturising effect of Aminoflora Moisturising, is one of the most important functions of cosmetics. In fact, moisturising is an effect advocated by almost all cosmetics. It is well known that neglecting moisturising can lead to dry skin, itchiness, roughness, abnormal
June 2024 PERSONAL CARE The final concentration of glucose and
glycerin was prepared to 35 mmol/L, which is equivalent to Aminoflora 3.3% (active ingredient concentration 1.0%). Place sterilized filter paper (φ5.5 mm) on the agar medium, inoculate 5 μL of bacterial solution (0.9 x 106
cfu/dot) onto the
filter paper, and culture at 30°C for seven days. The results indicate that Aminoflora cannot
be utilized by M. furfur, but glucose and glycerin promote the growth of M. furfur (Figure 5).
Capacitance (Δ)
Moisture
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