64 SKIN MICROBIOME
1.20 1.00
0.80 0.60 0.40 0.20 0
** ** ** ** * * Low
0H ■ 24H■■ 48H■■ n=3, ** p<0.01, * p<0.05 (t-test) significant from control
High **
microorganisms which as a consequence proliferate. In general, if the substances present on the skin provide nourishment or a favourable environment for good microorganisms, it can contribute to maintaining a healthy skin microbiome. The presence of substances that
promote the growth of bad/opportunistic microorganisms on the skin can disrupt the balance of the skin microbiome and potentially lead to various skin issues and conditions. We have conducted experiments using different types of microorganisms to verify the effectiveness of Aminoflora in regulating the balance of the skin microbiome. In order to confirm that Aminoflora can have
a positive effect on the skin microbiome while providing long-lasting moisturisation, we used some other moisturisers for comparison.
Figure 2: Measurement of C. acnes increase
0.14 0.12 0.10 0.08 0.06 0.04 0.02 0
0H ■ 24H■■ 48H■■ n=3, ** p<0.01, * p<0.05 (t-test) significant from control
High ** * *
Utilization of opportunistic microorganisms Cutibacterium acnes (C. acnes) is one of the most prevalent microorganisms that forms the human skin microbiome.8
Therefore, it is very
C. acnes can preserve
the balance of skin microbiome to help the skin to keep its good condition. However, under certain conditions it can cause inflammations and induce acne.8,9
**
important to inhibit the overgrowth of C. acnes in order to maintain the microbiome balance of the skin. We conducted experiments to demonstrate the utilization of Aminoflora and the other moisturiser for C. acnes. We mixed GAM semisolid medium containing 1% of the sample with C. acnes (NBRC107605) and cultured at 37°C for 48 hours under anaerobic conditions. Optical density (OD620) was measured
Low
immediately after inoculation, 24 hours, and 48 hours later. The results indicate that Aminoflora cannot be utilized by C. acnes, but glycerin and sorbitol promote the growth of opportunistic microorganisms, C. acnes (Figure 2).
Figure 3: Measurement of S. epidermidis increase
0.14 0.12 0.10 0.08 0.06 0.04 0.02 0
0H ■ 24H■■ 48H■■ n=3, ** p<0.01, * p<0.05 (t-test) significant from control
High ** ** * * ** **
Utilization of good microorganisms Staphylococcus epidermidis (S. epidermidis), microorganisms found in number on healthy skin, are considered as a key member of the healthy skin microbiome.10
They keep the
skin slightly acid and produces antibacterial substances which prevent the proliferation of bad microorganisms such as S. aureus.11 We did experiments to demonstrate the utilization of Aminoflora and the other moisturiser for S. epidermidis. S. epidermidis (NBRC100911) transferred to
SCD agar medium and incubated at 37°C. 4 mL of the following BPW (Pepton 10.0 g/L, NaCl 5.0 g/L, Na2
HPO4 9 g/L, KH2 PO4 1.5 g/L) medium
was mixed with 0.5% BPW medium containing 10% carbon source and 0.5 mL of each bacterial solution (1-9×107
cfu/mL) and incubated at Low
37°C. Optical density (OD620) was measured immediately after inoculation, 24 hours, and 48 hours later. The results indicate that Aminoflora and
Sodium PCA can be utilized by S. epidermidis and proliferate (Figure 3).
Figure 4: Measurement of S. aureus increase PERSONAL CARE June 2024
Utilization of bad microorganisms Research shows that excessive proliferation of
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OD 620
OD 620
OD 620
Increase in S. aureus
Increase in S. epidermidis
Increase in
C.acnes
Control Amitose R-PD Aminoflora Butylene Glycol Glycerin Sorbitol Sodium PCA Hyaluronic Acid
Control Amitose R-PD Aminoflora Butylene Glycol Glycerin Sorbitol Sodium PCA Hyaluronic Acid
Control Amitose R-PD Aminoflora Butylene Glycol Glycerin Sorbitol Sodium PCA Hyaluronic Acid
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