58 ANTI-AGEING
50 40 30 20 10 0
66 42.0% 62 58 12.7% 54 2ppm EGT Pure Figure 4: Evaluation of the antioxidant capacity of EGT Pure
chemical synthesis and the high-cost biological extraction with low content, Bloomage Biotech adopts a one-step biological synthesis method using a bio-based carbon source for fermentation. It has no residual toxic chemical reagents and complies with the green and sustainable trend of natural products. In terms of antioxidant capacity, the DPPH
radical is a highly stable free radical and an important indicator for evaluating antioxidant activity. EGT Pure at a concentration of 6ppm shows a remarkable DPPH radical scavenging rate of 41.96%. Besides, UVA plus UVB radiation stimulates
the production of ROS in cells. At a concentration of 0.005%, EGT Pure can eliminate 65% of ROS, thereby reducing oxidative damage caused by UV radiation (Figure 4). Therefore, EGT Pure has a comprehensive anti-ageing effect.
Minitrue: endogenous synthesis of HA, ceramide, collagen HA is a well-known substance, and there are many products of this kind available in the market. Most of the commercial products of HA are in the form of sodium salt, known as sodium hyaluronate. Minitrue is the new generation of
micromoleccular HA with a molecular weight of less than 1000 Da, which is produced by a patent enzyme degradation technology. The β-1, 3-glycosidic bond in HA was hydrolyzed to produce a series of saturated oligosaccharides with a reducing terminal of glucuronic acid. Minitrue can penetrate the skin surface
quickly, reach the dermis, stimulate endogenous vitality, promote the synthesis of endogenous ceramide, collagen and HA. Ceramides are the main components of
intercellular lipids in the stratum corneum, along with cholesterol and fatty acids. They form an important skin barrier and are present in all eukaryotic cells. Ceramides play a crucial role in regulating cellular differentiation, proliferation, apoptosis, ageing, and other life processes.12 Skin ageing begins with a decrease in the
hydration capacity of the stratum corneum, a decline in barrier function, and a slowing of the renewing of the stratum corneum. During the ageing process, lipid synthesis decreases, leading to a reduction in the content of
PERSONAL CARE January 2024
ceramides in the stratum corneum. Therefore, increasing the content of ceramides in the skin can help build a protective barrier from the outermost layer. Ceramides play a crucial role in connecting
protein-rich corneocytes, making them essential for the epidermal anti-water evaporation barrier. For example, the main component of vernix caseosa on the surface of baby skin is ceramides. It has numerous hydrophilic groups and a strong affinity for water, making them highly effective moisturizers. It can prevent the loss of water and
electrolytes, which is the fundamental function of ceramides. Studies have shown that even supplementing with a single component of ceramides can effectively reduce TWEL. Additionally, ceramides have anti-ageing effects as they can smooth out fine lines and wrinkles caused by dehydration. They can fill the gaps between skin cells, making the skin softer and more moisturized. Another great benefit of this ingredient is its suitability for all skin types, as it is naturally present in the skin. Although our skin produces ceramides, our
daily activities can deplete them. Some factors that contribute to this depletion are the use of irritating ingredients on the skin and excessive exfoliation. Therefore, replenishing ceramides is crucial for maintaining a skin-immune balance. Experiments by Bloomage have shown
that treatment with Minitrue at concentrations of 0.1%, 0.3%, and 0.5% results in a significant increase in the expression of ceramide synthesis genes (Cers) and the content of acid sphingomyelinase (aSMase) in a 3D skin model. Even at a concentration of 0.1%, Minitrue increases the ceramide content by 85.73%, indicating its ability to promote endogenous ceramide synthesis. Hyaluronan synthase (HAS) is a class
of enzymes that are located on the plasma membrane and specifically involved in the synthesis of hyaluronic acid (HA). There are three isoforms encoded by the genes HAS1, HAS2, and HAS3.13
Experiments have shown that treatment with
a 0.1% concentration of Minitrue significantly increases the expression of HAS1 and HAS3 genes in fibroblast cells. Fluorescence assay
results have also demonstrated a significant increase in HA content in the 3D skin model after treatment with 0.1% and 0.3% Minitrue. Furthermore, 0.001% Minitrue promotes the
synthesis of collagen type I in human dermal fibroblasts. Therefore, Minitrue enhances skin moisturization, strengthens the epidermal barrier, improves the anti-ageing capabilities of the dermal layer, and facilitates efficient transdermal absorption, ultimately promoting endogenous repair processes (Figure 5).
Conclusion In summary, skin ageing is caused by both internal and external factors. Primarily, Bloomage Biotech utilizes natural green fermentation methods and targets multiple anti-ageing pathways to strengthen the barrier of the skin, providing protection against pollutants and UV radiation. Secondly, natural antioxidant ingredients help
eliminate excess free radicals in the skin. Finally, the ultra-small molecules penetrate deep into the skin, providing enhanced moisturization and achieving comprehensive anti-ageing effects across all layers of the skin.
References 1. Tobin DJ. Introduction to skin aging. Journal of Tissue Viability. 26.1 (2017): 37-46
2. Krutmann J et al. The skin aging exposome. Journal of Dermatological Science. 85.3 (2017): 152-161.
3. Naylor EC, Watson REB, Sherratt MJ. Molecular aspects of skin ageing. Maturitas. 69.3 (2011): 249-256
4. Eyerich S, Eyerich K, Traidl-Hoffmann C, Biedermann T. Cutaneous Barriers and Skin Immunity: Differentiating A Connected Network. Trends in Immunology. 39(4), 315–327 (2018)
5. Elias PM. The brick and mortar model of the stratum corneum structure in mammalian skin. Journal of Investigative Dermatology. 80(1), 44-49 (1983).
6. Kaipeng J, Lim K. Why is autophagy important in human diseases? Experimental & Molecular Medicine. 44.2 (2012): 69
7. Martens S, Fracchiolla D. Activation and targeting of ATG8 protein lipidation. Cell
www.personalcaremagazine.com 6ppm 50 0.005% 0.050% EGT Pure 0.100%
65.22% ***
61.96% **
Cells: HaCat cells ***P<0.001
62.94% ***
PC
DPPH scavenging rate %
ROS scavenging rate %
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