HAIR CARE


4 3 2 1


0


Keratin Associated Protein 5 (KAP5)


A


No Treatment■ Plant C-Stem Bamboo ■ B Hair loss (Wash Test)


Plant C-Stem Bamboo ppm 0


10 Figure 4: KAP5 mRNA expression


increased keratin synthesis and nutrient intake for the new hair. Hair keratin-associated proteins (KAP) are


the major components of the hair fibre. Like all fibrous proteins, they perform a structural function. Hair resistance comes from the process of keratinization, where the cells reaching the final stage of maturation, lose the nucleus and fill with keratin. This is why keratin is important for strong, shiny, and soft hair.3,4 KAP5 is an age-related keratin protein that has been demonstrated to decrease with age. This diminution is correlated with a decrease in hair strength5


100


50 40 30 20 10 0


*** T0 T84 Figure 5: In vivo test (A) hair loss. (B) hair density


enrolled in the study is washed in a sink by covering its drain with gauze and then collecting and counting lost hair. The subject is taught to avoid shampooing for two days before the set test date. Compared to the baseline value (T0), a


significant decrease of 18% in the number of hair lost during the wash test was observed after 84 days of treatment. The use of the bamboo vacuolar extract has a significant effect in reducing hair loss during washing (Figure 5A). Hair density (number of hair/cm2


) was . The mRNA expression of KAP5


has been evaluated by exposing HHFK (1X105 cells/well) to the bamboo vacuolar extract for 24 hours. Some 100ppm of the active ingredient is enough to increase by three times the mRNA expression level of KAP5 (Figure 4).


In vivo studies In vivo tests have been carried out to evaluate hair loss (wash test) and density. The panel was composed of 20 subjects (males and females 30-60 years old) with seasonal hair loss. A lotion containing 2% of the bamboo vacuolar extract was applied three times a week for 12 consecutive weeks on the scalp (after washing) and gently rubbed. Subsequently, the hair has been dry. Evaluations of the primary (T0) and


secondary endpoints after 84 days (T84) have been done. The data on the quantitative discrete endpoints were described using the normal position and dispersion measurements: mean and standard deviation-median and interquartile range. A significance level of <0.05 was considered. During the wash test, the hair of the subjects


16 14 12 10 8 6 4 2 0


measured by HIROX – RX 2000 3D, a 3D digital microscopy system. Hair density analysis is carried out by acquiring images of the scalp and counting the number of hair in a 1cm2


area. Every measurement collected is the average


of hair density calculated in five different areas of the scalp. A statistically significant increase in hair density (+3%) between T0 and T84 has been observed. The treatment with the bamboo vacuolar extract has a significant effect on the growth of new hair (Figure 5B).


Ex vivo studies Ex vivo evaluation has been done to investigate the possibility of hair growth using the bamboo vacuolar extract. The hair follicles isolated from the human body


were separated and stored in Earle’s balanced salt solution (EBSS; Sigma Aldrich). Anagen phase follicles were separated using a stereomicroscope (Olympus, Tokyo, Japan) to avoid any damage. A total of three tests were carried out. Six


to 12 follicles were separated and used for the experiment. The isolated hair follicles were cultured in Williams medium E (Gibco, Grand Island,


* p<0.05 vs Control *** p<0.001 vs Control


*** * ***


NY, USA) with 2 mM L-glutamine (Gibco, NY, USA), 10 μg/ml insulin (Sigma Aldrich), 10 ng/ ml hydrocortisone (Sigma Aldrich), 100 unit/ml penicillin, and 100 μg/mL streptomycin at 37°C and 5% CO2


. On the first day of hair follicle culture, the


photographs were taken and the shape and length were confirmed. The hair follicular tissues were treated with the bamboo vacuolar extract (250 ppm and 1000 ppm) for seven days. Minoxidil (10μM) was used as a positive control. After seven days, the follicular tissues were


fixed in 10% neutral buffered formalin (NBF). The hair follicle elongation was analyzed using Image J (version 1.52a, NIH, Bethesda, MD., USA). Hair treated with the bamboo vacuolar extract 1000 ppm showed the same results as the drug Minoxidil in promoting hair shaft elongation in hair follicles (Figure 6).


Conclusion Plant C-Stem™ Bamboo stimulates the expression of main skin components providing hydration and helping regenerate the hair structure. According to in vitro studies, the bamboo vacuolar extract promotes the viability of essential hair components (HHFK and HHFDPC) and prevents hair ageing increasing KAP5 expression. Clinical studies demonstrate that the


active ingredient helps in reducing hair loss and increasing hair density, while ex vivo experiments comparing the activity of the bamboo vacuolar extract with the drug Minoxidil showed the same efficacy in promoting hair shaft elongation.


228 226 224 222 220 218


Hair density ** p<0.01 vs T0


** *** p<0.001 vs T0


43


T0


T84


PC


References 1. Buffoli B, Rinaldi F, Labanca M, Sorbellini E, Trink A, Guanziroli E, Rezzani R, Rodella LF. The human hair: from anatomy to physiology. Int. J. Dermatol. 2014 Mar;53(3):331-41


2. Trüeb RM, Henry JP, Davis MG, Schwartz JR. Scalp Condition Impacts Hair Growth and Retention via Oxidative Stress. Int. J. Trichology. 2018 Nov-Dec;10(6):262-270


3. Trüeb RM, Rezende HD, Dias MFRG. A Comment on the Science of Hair Aging. Int. J. Trichology. 2018 Nov-Dec;10(6):245-254


Control


Plant C-Stem Bamboo 250 ppm


Figure 6: Hair shaft elongation in hair follicles www.personalcaremagazine.com


Plant C-Stem Bamboo 1000 ppm


Minoxidil 10µM


4. Shimomura Y, Masaaki I. Human Hair Keratin- Associated Proteins. J. Investig. Dermatol. Symp. Proc. 2005 Dec;10(3):230-3


5. Hagihara M. Shiseido Global Innovation Center. Hair care Technology to make hair beautiful and healthy. J. Soc. Cosmet. Chem. Jpn. Vol.50, N.1, 2016


January 2024 PERSONAL CARE


Hair elongation %


Relative mRNA expression


Number of lost hair


Number of hair/cm2


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