54 ANTI-AGEING
of CPDna on the skin’s circadian rhythm: Fresh human skin with adipose tissue sheet. Donor: female, 60 years old, Caucasian, phototype IV. The skin explant was treated with and without CPDna (1%). Topical application on three consecutive day (day 1-3). After last application (day 3): UV irradiation (1 MED, 60 mJ/cm2
UVB, 1.14 J/cm2 UVA). Analysis of
gene expression of day 4. Green column: Gene expression of non-irradiated and non-treated skin is set at 0%. Red column: Gene expression of irradiated and non-treated skin is set at 0% (Figure 2). From the above-described results it can be
concluded that CPDna is able to compensate for the negative effects of UV radiation on the skin cells’ ability to repair DNA. This was assessed in a further cell biological experiments where the focus was on UV- induced CPDs. HaCaT keratinocytes were pretreated with
active ingredients for 48 hours. Then cells were irradiation with 1.5 J/cm2 UVA, 0.15/cm2
UVB.
Analysis with ELISA took place after irradiation. Use level of active ingredients: 1%. Non- irradiated control is set at 100%. Control was not treated with active ingredient. The results are shown in Figure 3.
Conclusion Due to evolutionary flaws, we, as humans, are not equipped to age well. Nature shows us how to age well and healthily, however: maintaining DNA integrity. For anti-ageing skincare, this is especially important as the exposome has a large negative effect on DNA integrity. UV radiation from the sun is by far the most important member of the exposome in this context. G+C Complex CLR™ (INCI: Bifida Ferment
Lysate) is a cosmetic active ingredient which represents decades of skin anti-ageing research by making use of postbiotics, i.e. lysates of probiotic bacteria for the benefit of the skin’s immune system. It is the latest product based on this
research and acts on UV-induced reduction of the ability to repair DNA, immunosuppression and dysregulation of the skin cells’ circadian rhythm. Consequentially, G+C Complex CLR is indeed able to help skin cells in DNA repair. Other experiments which are not shown
here, provide further proof of its relevant to anti-ageing skincare. Downstream negative effects of persistent DNA damage, such as the microinflammatory processes that take place leading to visible signs of skin ageing, i.e., ‘inflammaging’ are significantly reduced. Also, cell vitality is maintained, despite UV irradiation.
250 200 150 100 50 0
Irradiated
Irradiated Non-
Control Figure 3: G+C Complex CLR reduces UV-induced DNA damage PC
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G+Complex CLR
CPD mutations (%)
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