SKIN MICROBIOME


55


120 100 80 60 40 20 0


0h


24h


48h


3-Hydroxy-3-methyl- hexanoic acid (HMHA)


Indole Diphenyl Oxide


3-Methyl-2-Hexenoic Acid (3M2H)


Valeric Acid 2-Methylbutyric Acid Butyric Acid Isobutyric Acid Propanoic Acid Acetic Acid


O OH


3-Methyl-2-hexanoic acid (3M2H)


OH O OH


3-Hydroxy-3-methyl- hexanoic acid (HMHA)


SHORT CHAIN FATTY ACIDS


Figure 3: Chemical analysis of the untreated and treated sweat samples


complementary complexity of the sweat malodour we have exploited our previously developed ex vivo 3D model and conducted an in vivo study with a candidate ingredient for deodorant application. First, a microbiological analysis including sequencing was performed with sweat samples. The amplicon 16S rRNA gene sequencing results demonstrate that Anaerococcus and


Staphylococcus are the most abundant genera in untreated human sweat (Figure 1). The addition of propanediol caprate to the


sweat samples showed a dose dependant slight reduction of Anaerococcus species which is more pronounced with increasing amounts of active (5000 ppm versus 3000 ppm); which indicates antimicrobial effectiveness. However, at the same time, the natural ratio


of a gram-positive dominated spectrum of microorganisms was kept. This is in contrast to the findings we made on sweat samples treated with triclosan in previous studies, in which most gram-positives were eradicated, and the mix of microorganisms was dominated by the gram- negative genus Pseudomonas afterwards.9 Thus, at the tested concentrations, the


overall composition of the microbiome is kept


www.personalcaremagazine.com


October 2024 PERSONAL CARE


ppm


Untreated


Untreated


PMD green 5000 ppm


SymDeo


Untreated


PMD green 5000 ppm


SymDeo


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