74 PEPTIDES 0ng/ml T-EGF 0h 24h 48h
10ng/ml hEGF
0h 24h 48h
Figure 3: (Left) Cells were treated with TD1-hEGF (10 ng/ml) and hEGF (10 ng/ml) after scratch was made for zero hours, 24 hours and 48 hours. Lane 0 ng/ml: untreated cell sample was negative control. Lane hEGF are native hEGF for standard. Microscopic images were captured after 24 hours and 48 hours. (Right) Dosage dependent scratch assay from adding 0 to 10 ng/ml TEGF. Microscopic images were captured at T0 and after 24 hours and 48 hours for each concentration. Figure is taken from published doctoral thesis
Chemical penetration Chemical penetration enhancers include using chemicals (e.g. surfactants) that alter the arrangement of lipids within the skin’s outermost layer. Their action induces swelling and softening of the stratum corneum, in turn, leads to enlargement of sweat gland and hair follicle openings. Through this mechanism, the enhancers facilitate the passage of molecules through the skin barrier, fostering transdermal absorption.
Though these substances exhibit a definite
capability to enhance skin permeability, there are two drawbacks: (a) tendency to cause skin allergies and (b) inability to significantly facilitate the transdermal delivery of hydrophilic macromolecules e.g. proteins.12
Physical assistance Physical assistance includes iontophoresis, ultrasonic, microneedle, electroporation, and laser treatment. These methods are effective to deliver a diverse array of substances. However, they are not well-suited for wide home usage due to the necessity of specialized equipment and limited flexibility in forms of dosage, all while inducing various degrees of pain and discomfort.
A novel biological approach – TD1 transdermal peptide In 2006, Professor Wen Longping and his team of the University of Science and Technology of China applied ‘in vivo phage display technology’ to the field of transdermal drug delivery, and successfully discovered a short transdermal peptide composed of 11 amino acids, which can transport a variety of molecules, including large protein polymers, through the skin effectively.13 The transdermal peptide acts on the hair
follicle to temporarily open the skin barrier, assists macromolecular substances to pass through and reach the subcutaneous tissue.13 Professor Mark R. Prausnitz, a respected
international expert, published commentary citing this work: “Chen et al have taken
PERSONAL CARE November 2023 Average
600 500 400 300 200 100 0
hEGF **P<0.001 Sample ◆
0
1
5
10ng/ml
*P<0.05
TD1-hEGF
TD1-hEGF+ATP
Figure 4: T-EGF Transdermal in human skin. TD1-hEGF+ATP,TD1-hEGF and hEGF (50µg/mL) were administered to human skin separately for 16 hours. hEGF levels were measured by ELISA kit. Mean±s.e.m. (n=4) *P < 0.05. **P < 0.0011
a biological approach to increase skin permeability, in contrast to the chemical and physical approaches previously investigated… protein delivery from a patch with equal efficacy and similar cost would almost certainly make hypodermic injection of many proteins obsolete. This prospect is what makes finding a peptide chaperone for transdermal delivery such an exciting advance.”14
The introduction of a transdermal EGF Following the discovery of TD1, Longsheng Biotech created a fusion of TD1 and EGF. TD1- EGF (T-EGF) not only retains the same biological efficacy in promoting skin cell growth as conventional EGF but also boasts a transdermal efficiency that surpasses that of traditional EGF by over ten times.15 The beneficial efficacy to cell activity,
repairing and moisture effects demonstrates, among others, how this remarkable enhancement in transdermal efficiency
significantly widens the scope of EGF application within the cosmetics industry.
Cell activity and transdermal efficiency The ability of T-EGF to promote the migration of balb/c 3T3 cells was demonstrated through cell scratch experiment.15
The experimental results
showed that, in the presence of 10 ng/mL T-EGF or human recombinant EGF (hEGF), after 24 hours of cell culture, cells migrated towards the centre of the scratch. In comparison to the control group, following
48 hours of continuous culture, a significant number of cells migrated to the scratch’s centre when treated with 10ng/mL T-EGF, resembling the response seen with hEGF. The promotion of cell migration by T-EGF exhibited a concentration-dependent manner, with a higher concentration of T-EGF corresponding to a stronger ability to induce cell migration. The results unequivocally demonstrated that the activity of promoting cell migration is similar
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Amount of permeated protein (pg)
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