42 ANTI-POLLUTION
Figure 2: The APG microalgae sustainable greenhouse plant
some microalgae species would be of a great potential as a bioremediation option not only for CO2
but also for greenhouse gases and other
pollutants. A unique patented sustainable technology
is used for our microalgae production. Green energy is produced by using the entire organic production waste and transforming them into biofuel, thanks to an on-site power station. Ninety percent of the water used during the cultivation is reused, maintaining integrity and quality of the farming environment. Finally, this patented production technology has a positive impact on the environment: carbon dioxide is directly converted into oxygen by the microalgae, resulting in a final negative carbon footprint, in other words, a positive oxygen release. Thanks to an extensive production capacity
of more than 30 tonnes per year of dry microalgae biomass and a strong expertise in natural extraction, interesting active molecules can be isolate from the biomass, resulting in a fully natural and sustainable extract, targeting high levels of proteins, carotenoids, essential poly-unsaturated fatty acids, vitamins, or antioxidants.
The Pavlova lutheri microalgae: an active composition preventing pollution damage To counteract the pollutant effect and environmental stress, Natura-Tec Biotechnology Center has developed a specific microalga active, Natura-Tec Marine CellShield AP™,
35% 30% 25% 20% 15% 10% 5% 0%
hereafter known as the Pavlova lutheri active, based on the Pavlova lutheri microalgae species.
This species takes its name from the shape
of its cell with two characteristic flagellums and inspired by the famous Russian ballerina Anna Pavlova, who performed in the late 18th and early 19th century and was known for her elongated and delicate legs. This species was selected due
to its high content in omega-3, long chain eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), carotenoids and sterols, to help the body fight against the oxidative stress of PM (particulate matter) and help to restore the skin barrier function. Different studies found out that omega-3
fatty acids EPA and DHA can protect skin health against exposure to ambient PM2.5 in areas with severe air pollution,9
inhibiting
PM2.5-related inflammatory reactions by affecting arachidonic acid metabolism and further playing a protective role against the development and progression of chronic inflammatory-related diseases.10 Microalgae can produce the most powerful
carotenoids found in nature. The carotenoids synthetized by the Pavlova lutheri microalgae, are able to boost skin’s innate resistance against ultraviolet (UV) B-induced erythema (sunburn), to provide photoprotection against UVA- induced pigmentation and to inhibit molecular markers of oxidative stress.11 Microalgae carotenoids are more effective at reducing ferric ions than synthetic antioxidants,
25% p<0.01
14% p<0.05
17% p<0.05
29% p<0.01
33% p<0.01
0% CTL+ (0%) 0.1% 0.2% 0.5% 1.0% Pavlova Lutheri Extract concentration Figure 3: NHEK cell viability variation under indoor dust PERSONAL CARE November 2023 2.0%
160% 140% 120% 100% 80% 60% 40% 20% 0%
with strong DPPH-scavenging activity, with a protective effect on the viability of stressed epidermal cells and quenching reactive oxygen species induced by hydrogen peroxide or cigarette smoke.12 Sterols from microalgae exhibits a broad
range of effectiveness against ROS production, inflammatory responses, and cell damage induced by PM. Due to its active composition, the efficacy of the Pavlova lutheri microalgae active has been evaluated with in vitro and in vivo efficacy tests, to demonstrate its major role to neutralize the activity of harmful pollutants.
In vitro protection against urban and indoor dust The evaluation of the protective effects of Pavlova lutheri active was studied at different concentrations against urban or indoor pollutants on normal human epidermal keratinocytes (NHEK). Indoor and urban dust have a negative impact on cell viability: pollutants decrease cell viability respectively of 48% and 51%.
With the use of the Pavlova lutheri active, an immediate protective effect is observed, against indoor and urban pollution, with a significant increase observed already with 0.1%, the minimal dose tested. Looking at urban pollution, from 0.5% of use, a total inhibition of pollution damages is observed. Moreover, at this dosage, the cell viability
observed, is greater than in the non-intoxicated condition (without pollution), indicating that viability and cell stimulation are substantially
134% p<0.001
99% p<0.01
67% p<0.001
134% p<0.001
134% p<0.001
0% CTL+ (0%) 0.1% 0.2% 0.5% 1.0% Pavlova Lutheri Extract concentration Figure 4: NHEK cell viability variation under urban dust
www.personalcaremagazine.com 2.0%
Cell Viability variation (%), vs. no active under indoor pollutants
Cell Viability variation (%), vs. no active under urban pollutants
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