ANTI-POLLUTION At the end of the application, the metabolic
activity of the cells was quantified as conversion of the MTS salt.
Test system HaCaT cells HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin. The cell line was routinely cultured in laboratory in DMEM medium supplemented with glutamine, antibiotics and fetal bovine serum (complete medium).
Test methods Cells from routine culture were seeded into 96 well plates in serum-free DMEM medium and allowed to attach overnight. The day after, cells were exposed to dilutions (0.01 to 1% v/v) of the strawberry active in serum-free medium. As comparison, glycerin at the same
concentration of each strawberry active dilution was prepared and used as third treatment. After 24 hours of exposure to the strawberry active or glycerin, medium from each well was replaced with complete medium without phenol red and the MTS assay was applied to measure cell viability. To analyze the results of the test, an unpaired
t-test was applied to compare the means of two groups (in our case each strawberry active concentration vs the respective glycerin concentration). The tests were performed by LabAnalysis of Casanova Lonati, Italy.
Results The results of the study with the strawberry active are reported in Table 3 and Figure 3. Activity of the cells was normalized on the activity of untreated cells as control group. Fetal bovine serum at 10% v/v was used as
positive control, acting as stimulus for the cells. Cells treated with the strawberry active showed higher activity compared to the unexposed control group and to glycerin. A dose-response trend was found in cells exposed to the strawberry active. The comparison between cells exposed to
the strawberry active and to glycerin showed statistically significant differences in most of the concentration tested.
TABLE 2: ANTIOXIDANT EFFECT OF THE STRAWBERRY ACTIVE AT 0.5% V/V. RESULTS ARE EXPRESSED AS RELATIVE FLUORESCENCE UNIT (RFU)
NO STRAWBERRY ACTIVE MENADIONE (µM)
20 50
100
6000 5000 4000 3000 2000
MEAN
4298.5 4775 5349
STD.
DEVIATION 208.597
70.491 205.061 MEAN
3425.333 4077.5 4434
0.5% STRAWBERRY ACTIVE STD.
DEVIATION 34.429
95.459 174.253
STATISTICAL SIGNIFICANCE (P VALUE)
0.0020 0.0005 0.0042
EFFECT REDUCTION%
20.31 14.61 17.11
Untreated ■ 0.5% Strawberry Active ■
39
20
50 Menadione (µM) Figure 2: Effect of the strawberry active on HaCaT cells
Conclusion The results of these in vitro studies are very promising and demonstrate the ideal application of the strawberry active in anti-pollution cosmetic products, where an antioxidant action is sought, together with a booster effect on cell vitality. Its effectiveness, together with its ability
to give the cosmetic product a completely natural colour and fragrance, make it the perfect ingredient for a clean beauty routine.
References 1. Abolhasani R, Araghi F, Tabary M, Aryannejad A, Mashinchi B, Robati RM. The impact of air pollution on skin and related disorders: A comprehensive review. Dermatol. Ther. 2021 Mar;34(2):e14840. doi: 10.1111/dth.14840
TABLE 3: EFFECTS OF THE STRAWBERRY ACTIVE ON HACAT CELLS. RESULTS ARE EX- PRESSED AS PERCENTAGE VIABILITY NORMALIZED ON CONTROL UNEXPOSED GROUP
115 TREATMWENT STRAWBERRY ACTIVE % CTR CONTROL
Concentration of strawberry active or glycerin (%volume)
100 SD% 2.910561 GLYCERIN % CTR - SD% -
STATISTICAL SIGNIFICANCE
P-VALUE SIGNIFICANT 102.5 0.01
0.05 0.1
0.5 1
10% FBS 105.5553 0.909143 102.8535 0.849489 0.0198
106.7738 0.272668 103.7275 0.763457 0.0029 107.4807 2.588243 104.5758 0.327196 0.126 107.9434 1.911499 104.0745 0.927055 0.0344 109.8458 0.893845 104.3445 2.975915 0.0374 176.9409 5.899798
-
www.personalcaremagazine.com - - YES 96.25
YES NO YES YES -
90 108.75 PC
2. Puri P, Nandar SK, Kathuria S, Ramesh V. Effects of air pollution on the skin: A review. Indian J. Dermatol. Venereol. Leprol. 2017 Jul-Aug;83(4):415-423. doi: 10.4103/0378- 6323.199579
3. Giampieri F, Forbes-Hernandez TY, Gasparrini M, Alvarez-Suarez JM, Afrin S, Bompadre S, Quiles JL, Mezzetti B, Battino M. Strawberry as a health promoter: an evidence-based review. Food Funct. 2015 May;6(5):1386-98. doi: 10.1039/c5fo00147a
4. Tsai TH, Huang CJ, Wu WH, Huang WC, Chyuan JH, Tsai PJ. Antioxidant, cell- protective, and anti-melanogenic activities of leaf extracts from wild bitter melon (Momordica charantia Linn. var. abbreviata Ser.) cultivars. Bot. Stud. 2014 Dec;55(1):78. doi: 10.1186/s40529-014-0078-y
Strawberry Active ■ Glycerin ■
100
CTR 0.01 0.05 0.1
0.5 % of the treatment (v/v) Figure 3: Effect of the strawberry active on HaCaT activity November 2023 PERSONAL CARE
1
Fluorescence (RFU)
Avtivity vs CTR
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