28 SKIN CARE VOLUNTEER #04 WOMAN AGED 53 YEAR-OLD WITH DEHYDRATED DRY SKIN TYPE PLACEBO D0 D28 D0 GSFE 1% D28
NO VISIBLE CHANGE
have shown that two specific species, S. epidermidis and Ralstonia sp abundances were upregulated by the active treatment. S. epidermidis has a well-known role in skin health and barrier function,5
VISIBLE REDUCTION OF WRINKLES Figure 4: Illustrative pictures of wrinkles for one volunteer with dehydrated and dry skin after 28 days of treatment with GSfE (right) or placebo (left) and Ralstonia sp
is increased in skin defined as ‘ideal’, which have a higher hydration content compared to undesirable skin.6
Consequently, upregulating
abundance of these two bacteria, respectively by +39% and +45% versus D0, strengthens barrier function for skin moisture maintenance. Comparison between older dehydrated
and younger hydrated panel at D0 highlighted that with age and dehydration, barrier function and antioxidant cell responses are slowed down in the skin holobiont. Indeed, there is a downregulation of skin barrier preservation and antioxidant activity, while oxidative stress is upregulated in dehydrated older group compared to younger individuals. As a solution to dehydration, our upcycled
active restores youthful protein functions by upregulating proteins linked to skin barrier preservation and antioxidant responses, while downregulating proteins involved in oxidative stress in older dehydrated skins after 56 days of treatment versus D0. Volunteers treated with GSfE showed also an improvement of natural
Down-regulation Up-regulation
Skin barrier preservation* Antioxidant*
Oxidative stress*
Natural defences* DNA damage repair*
*p<0.05 D56 vs D0
Figure 5: Significantly regulated proteins clustered in main functions in older dehydrated volunteers’ group after 56 days of treatment with GSfE 1% versus D0. *p<0.05 versus D0
PERSONAL CARE June 2023
skin defenses and DNA damage repair, by an upregulation of associated proteins. Thus, thanks to an emerging technology, it has been shown that GSfE is able to respect microbiota diversity while invigorating skin holobiont. It participates to reinforce skin barrier and thus to lock in skin moisture.
Conclusion To summarize, to counteract dehydrated (lack of water) and dry (lack of lipids) skin, GSfE boosts HA synthesis and protection against free radicals’ degradation, both in the epidermis and the dermis. It also stimulates HA receptor CD44 and its signaling cascade allowing the reinforcement of epidermal barrier. Indeed, tight junction and differentiation markers are upregulated as well as lipid synthesis in keratinocytes for a more cohesive epidermis able to lock water within the skin. GSfE also stimulates DEJ markers expression for a better diffusion of free water within the skin. Greentech R&D goes a step further by studying the skin holobiont thanks to a metaproteomic study. Slow-down of skin barrier and antioxidant functions both from human and microbial origins observed in dehydrated older skin can be stimulated by GSfE for an invigorated
Dehydrated older D0
1% D56 GSfE
skin holobiont. Moreover, it is a microbiota- friendly active which respects microbial diversity at genus level. However, it stimulates, at species level, abundances of Staphylococcus epidermidis and Ralstonia sp, known for their beneficial action on skin barrier and hydration. In conclusion, Holobiosys is a potent natural
and upcycled next-generation moisturizer able to bring a deep skin hydra-restructuration while invigorating its holobiont for a rehydrated and plumped skin.
References 1. Ebner F, Heller A, Rippke F, Tausch I. Topical use of dexpanthenol in skin disorders. Am. J. Clin. Dermatol. 2002;3(6):427-33. doi: 10.2165/00128071-200203060-00005. PMID: 12113650
2. Radner FP, Grond S, Haemmerle G, Lass A, Zechner R. Fat in the skin: Triacylglycerol metabolism in keratinocytes and its role in the development of neutral lipid storage disease. Dermatoendocrinol. 2011 Apr;3(2):77-83. doi: 10.4161/derm.3.2.15472
3. Feingold KR, Elias PM. Role of lipids in the formation and maintenance of the cutaneous permeability barrier. Biochim. Biophys. Acta. 2014 Mar;1841(3):280-94. doi: 10.1016/j. bbalip.2013.11.007
4. Velez-DelValle C, Marsch-Moreno M, Castro-Muñozledo F, Kuri-Harcuch W. Decorin gene expression and its regulation in human keratinocytes. Biochem. Biophys. Res. Commun. 2011 Jul 22;411(1):168-74. doi: 10.1016/
j.bbrc.2011.06.122
5. Zheng Y, Hunt RL, Villaruz AE, Fisher EL, Liu R, Liu Q, Cheung GYC, Li M, Otto M. Commensal Staphylococcus epidermidis contributes to skin barrier homeostasis by generating protective ceramides. Cell Host Microbe. 2022 Mar 9;30(3):301-313.e9. doi: 10.1016/
j.chom.2022.01.004
6. Ma L, Niu Y, Yuan C, Bai T, Yang S, Wang M, Li Y, Shao L. The Characteristics of the Skin Physiological Parameters and Facial Microbiome of “Ideal Skin” in Shanghai Women. Clin. Cosmet. Investig. Dermatol. 2023 Feb 3;16:325-337. doi: 10.2147/CCID.S400321
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