60 TRENDING TECHNOLOGIES
700000 600000 500000 400000 300000 200000 100000 0
Figure 4: CHARM NovaLum ATP analyzer
site. Using the statistical function in Microsoft Excel, the Student Paired, two-tailed T-Test was employed to determine 95% confidence (p≤0.05) for each time point compared against the untreated control site.
Results and discussion The results of the study protocol described above are shown in Figure 5. The RFU value for the untreated control sites was approximately 150,000. This value, as noted above, would be a combination of both the microbial contribution of ATP and the somatic (skin cell) contribution. After 20 minutes of application of the
freshly prepared emulsion containing the Lactobacillus, the RFU increased to approximately 450,000 in 20 minutes and rose to over 600,000 RFU within one hour after application of the cream. There are no ingredients in the cream formulation except for the Lactobacillus that could contribute additional ATP to the skin. The measurements indicate that
application of the cream increases the ATP content on the skin nearly threefold in 20 minutes and this level of ATP content remains constant but appears to continue to increase to nearly fourfold compared against the untested control site after 60 minutes. The 60-minute test site value, however, is
not statistically different from the 20-minute timepoint indicating the level of ATP remains relatively constant for at least one hour after application of the cream.
Conclusions The ATP biofluorescence testing indicates that application of the Lactobacillus emulsion increased the microbial content significantly and quickly and the site retained active microbial load for at least one hour after initial application. Keeping in mind that - based on the results of the earlier H2
O2 sanitizing study -
the skin’s original ATP levels will reconstitute at the skin’s surface within eight hours, the studies completed here suggest that in that interim timeframe - while the skin’s normal microbiome is diminished - the opportunity exists to present unique probiotics that might have beneficial effects on the skin. What some of these effects might be is
PERSONAL CARE June 2022 p-0.02 p-0.36 p-0.004
Baseline
T(1) 20 Min
T(2) 60 Min
Figure 5: RFU values comparing control/baseline (Blue), 20 minutes (light green) and 60 minutes (dark green) measurements after application of cream. (N=12, p≤0.05)
currently being investigated. In addition, the opportunity to examine how powders containing the living cultures might compare against similar just-add-water emulsions containing simple lysates or filtrates now presents itself as an opportunity for further investigation. Furthermore, while the studies presented
here were done around the Stratabiosys Technologies containing Lactobacillus, the opportunity to work with numerous other quiescent probiotic cultures is available and readily adaptable to the technology. BotanicalsPlus and Jeen continue to push
the envelope in their efforts to develop new and exciting cosmetics technologies and to lead the industry in presenting these new technologies with supporting scientific studies. The ideas presented here offer a first glimpses into a new, novel way to administer living microorganisms to the skin with point- of-use just-add-water technologies. A key limitation of the technology is that it must be used in single dose applications to prevent the unintended growth of contaminating microorganism after reconstitution of the just- add-water emulsions. This is a preservative-free, waterless
technology that puts the benefits and control of preparing the creams into the hands of the consumers. Where this might lead is open to the creativity of the industry. BotanicalsPlus looks forward to seeing how these advances evolve going into the future of skin and hair care technologies.
Dermatol. 2020;19:689-693
6. U.S. Patent 10,959,950 Stabilized Compositions for the Controlled Delivery of Probiotics and Methods and Production Thereof. Dakota Biotech 2021
7. Paetzold B, Willis JR, de Lima JP, Knodlseder N, Bruggemann H, Quist SR, Gabaldon T, Guell M. Skin microbiome modulation induced by probiotic solutions. Microbiome 2019;7:95
8. Callewaert C, Knodlseder N, Karoglan A, Guell M, Paetzold B. Skin microbiome transplantation and manipulation: Current state of the art. Comput Struct Biotech J. 2021;19:624-631
9. U.S. Patent Application 2019/0160117 Method and Topical Composition for Modification of a Skin Microbiome. Amway 2019
10. Onwuliri V, Agbakoba NR, Anukam KC. Topical cream containing live lactobacilli decreases malodor-producing bacteria and downregulates genes encoding PLP-dependent enzymes on the axillary skin microbiome of healthy adults. J Cosmet Dermatol. 2021;20:2989-2998
11. Wallen-Russell C, Wallen-Russell S. Meta analysis of skin microbiome: New link between skin microbiota diversity and skin health with proposal to use this as a future mechanism to determine whether cosmetic products damage the skin. Cosmetics 2017;4:14;doi:10.3390/ cosmetics4020014
12. Wallen-Russell C, Wallen-Russell S. A new benchmark to determine what healthy western skin looks like in terms of biodiversity using standard methodology. Cosmetics 2020;7:79;doi:10.3390/cosmetics7040079
PC
References 1. Yu J, Ma X, Wang X, Cui X, Ding K, Wang S, Han C. Application and mechanism of probiotics in skin care: A review. J Cosmet Dermatol. 2022;doi:10.1111/jocd.14734:1-9
2. Yu Y, Dunaway S, Champer J, Kim J, Alikhan. Changing our microbiome: probiotics in dermatology. Brit J Dermatol. 2020;182:39-46
3. Skin Microbiome Handbook. Dayan, N., Ed. Scrivener Publishing, Beverly, MA. 2020
4.
www.clinicaltrials.gov 5. Notay M, Saric-Bosanac S, Vaughn AR, Dhaliwal S, Trivedi M, Reiter PN, Rybak I, Li CC, Weiss LB, Ambrogio L, Burney W, Sivamani RK. The use of topical Nitrosomonsa eutropha for cosmetic improvement of facial wrinkles. J Cosmet
13. Gruber JV, Riemer J. Measuring the skin microbiome after a 3% aqueous hydrogen peroxide (H2O2) dysbiosis using ATP biofluorescence. Research Square Preprint 2022;doi: 10.21203/
rs.3.rs-1325039/v1
14. Liceaga A, Mercado E, Narcaroti M, Liceaga L. Hand hygiene skin ATP study: Hand washing with tap water and soap vs ozonated water and soap vs antiseptic and tap water vs antiseptic and ozonated water vs antiseptic and tap water. Becker’s Hospital Review. 2017 https://
www.beckershospitalreview.com/quality/ hand-hygiene-skin-atp-study-hand-washing- with-tap-water-and-soap-vs-ozonated- water-and-soap-vs-antiseptic-and-ozonated-
water-vs-antiseptic-and-tap-water.html
15. Gruber JV, Perle A. Accelerating product development through cold-water dispersible wax technologies. HAPPI 2018;May:2-4
www.personalcaremagazine.com
Average RFU
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