SKIN MICROBIOME 85 Normal keratinocyte Keratinocyte treated with SACM
Keratinocyte treated with SACM +2% ECOMUSHLYTE
Figure 2: Cell protection from damage caused by pathogen secretion.
interactions within the phytobiome, Dermalab has developed Ecomushlyte. Ecomushlyte is a hydrolysate of Reishi
mushroom (Ganoderma lucidum), Maitake mushroom (Grifola frondosa) and Shiitake Mycelium (Lentinus edodes) produced with a unique green extraction method called “Mycelysis ExtractionTM
”.
Maitake mushroom (Grifola frondosa) Also known as ‘hen of the woods’, the fruiting body of the maitake mushroom is used and is regarded as a medicinal mushroom in Asia. It is rare, and hence is also known as ‘jewel of the woods’. The mushroom contains polysaccharides which enhance the immune system.
Reishi mushroom (Ganoderma lucidum) Also known as ‘lingzhi’, the stem of the reishi mushroom has a long history of use as a promoter of health and longevity. It well known as having an anti-cancer effect and contains polysaccharides which have an immune system modulating effect.
Shiitake Mycelium (Lentinus edodes) The mycelium of the shiitake mushroom is used by people, but they also play an important role in the energy cycle within ecosystems. They release a digestive enzyme which breaks down organic compounds into soluble nutrients, and also form symbiotic relationships with plants.
Mechanism The microbiota present on the skin surface plays a major role in maintaining skin homeostasis. However, the balance of the microbiota on the skin is easily affected by pollution, ultraviolet rays, stress and the irregular lifestyle of modern society. The skin acts as an active immune
structure, and it forms an interactive network composed of microbiome, chemical, physical and immune barrier.
Microbiome barrier (Fig 2) The microbiome is an intricate ecosystem controlling pathogens and skin immunity. Increased population of pathogens such as S. aureus affects the metabolic activity of epidermal cells.
November 2020
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80
60
40
20
0 SACM – Control
+ –
+ 2% ECOMUSHLYTE Figure 3: Skin pH readjustment by increasing expression of chemical barrier factors.
Protocol Human epidermal keratinocytes (HaCaT) were incubated for 24 hours (at 37°C, 5% CO2
) in the presence of 2% Ecomushlyte
(now referred to as ‘the mushroom hydrolysate’) with S. aureus cultured medium (SACM) 36µg/ml. After incubation, changes in cellular morphology of each group were determined using microscopy (Olympus, Japan).
Results The result of the study shows that keratinocyte cells receive protection from damage caused by pathogen secretion as a result of the introduction of the active.
Chemical barrier (Fig 3) Secretory phospholipase A2 (SPLA2) contributes to epidermal barrier formation and homeostasis by generating free fatty acids, and is important in controlling skin pH level (acid mantle). It is an enzyme that hydrolyses membrane phospholipids. Harmful substances released by pathogens decrease the activity of sPLA2.
Protocol Human epidermal keratinocytes (HaCaT) were incubated for 24 hours (at 37°C, 5% CO2
) in
the presence of 2% mushroom hydrolysate with S. aureus cultured medium (SACM) 36µg/ml. The expression levels of mRNA were analysed by RT-PCR. LA: Lactic Acid.
Results The study looked at skin pH readjustment via increasing expression of chemical barrier factors and showed that levels of sPLA2 were increased by 18.6 % in the presence of the active.
Physical barrier (Fig 4) Keratinocytes are in charge of forming and preserving the solid structure of the skin. The production of epidermal lipids, ceramides, is essential for restoring damaged barrier and maintaining the skin structure. aSMase (Acidic sphingomyelinase) is
responsible for the conversion of a fat called sphingomyelin into another type of lipid called ceramide. GBA (β-Glucocerebrosidase) is a
PERSONAL CARE ASIA PACIFIC +
20uM LA
sPLA2 expression (%)
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