72 SKIN MICROBIOME
the volunteers’ brain activity was analysed by functional Magnetic Resonance Imaging (fMRI), a sophisticated technique that measures the changes in brain activity associated with changes in blood flow, which is associated with the neuronal activation of a specific area. This allows to detect which brain areas are
activated after applying the active and the relationships between different brain areas through the calculation of correlation coefficients. For this in vivo brain analysis, 132 brain
regions were analysed on the 20-volunteer subgroup to evaluate the brain connections after the application of a lotion containing 2% dosage of the active in order to understand how it activates in vivo the different brain pleasures areas of the participants. The study was carried out with a consortium between a Quantitative Imaging Biomarkers company and Hospital de la Fe in Valencia (Spain) and an ethical committee approval was required to conduct such study. A series of correlation coefficient
weighted matrices for every pairwise region of the brain was developed where red areas indicated strong brain activation and blue areas indicated a low brain activation. From such matrices, 3D brain models were elaborated to illustrate which brain areas were activated and connected (Fig 6). Compared to placebo, the results
showed that the active demonstrated a higher positive brain activation at 15 minutes than placebo after application, while producing an even higher positive activation of the brain pleasure zones at 28 days (Fig 7). The active demonstrated a higher
activation of regions and significant brain connections than placebo, being those associated with the touch sense and affective processes, especially being the anterior cingulate cortex (ACC) and
Before Vol.#28 After +
2 1 0
_
-1 -2
n Placebo cream n Cream containing 2% Kannabia Sense PLF
Vol.#26
35 30 25 20 15 10 5 0
+29% +18% +8.5% +8.5% Optimistic Joyful Cheerful Happy D0 (15 min) KANNABIA SENSE PLF
Inferior Temporal Gyrus Anterior Cingulate Cortex Pallidum Cerebellum Frontal orbital Cortex Vermis
D28
Superior Temporal Gyrus Middle Temporal Gyrus Anterior Insula Amygdala Subcallosal Cortex Vermis
Placebo
Frontal Gyrus Cerebellum Insular Cortex Hippocampus
Temoral Fusiform Cortex Default Mode LP
Figure 7: Clinical evaluation of the brain activation by fMRI.
pallidum zones (part of the “lentiform nucleus” region which in turn is part of the “striate body”)8
, the most relevant parts of
the study and, also, a considerable activation of the orbitofrontal cortex (OFC). It has been demonstrated that the
ingredient activates and connects the same brain regions both immediately and in the long-term the subcallosal cortex and the temporal gyrus with the amygdala. Furthermore, the palladium and the amygdala on one hand, and the anterior cingulate cortex and the subcallosal cortex on the other hand, are regions especially involved in the response to oxytocin and all located in proximity. The active showed an activation profile
like oxytocin, demonstrating its ability to activate the neuroendocrine skin-brain axis, similar to touch and massage and its emotional effectiveness. This activated brain pleasure regions that are related to a greater social predisposition and attractiveness of the person. l Modulation of mood state The emotions generated in the 30 volunteers after the application of a cream containing 2% of the active were also
analysed. The positive emotions of the volunteers increased up to 29% and the volunteers declared to feel more relaxed, confident and happier (Fig 8).
Conclusion The active ingredient Kannabia SensePLF
is
an innovative prebiotic treatment that stimulates the skin microbiota to produce a positive in situ postbiotic cocktail that ignites the synthesis of cutaneous oxytocin. This process activates the brain pleasure centers, induces a better self-perception and positive emotional parameters, what ends up in a healthier and prettier skin. Kannabia SensePLF
has a wide range of
applications within the cosmetic sector: well- ageing, anti-ageing, and delicate facial treatments, restoring night creams, and uplifting massage lotions, for men and women. This new Cannabis raw material based
on plant stem cells is a step forward in the area of neuro-cosmetics due to an innovative mechanism of action that brings new benefits for the human skin while being respectful to the skin microbiota.
PC
References 1 Misery L, et al. Pruritus, Chapter 34
Psychosomatics and Psychiatry 2010; 219.
2 Denda M. The epidermis as the third brain. Dermatologica sinica 2015; 33:70-73.
3 Bocheva G, et al. Neuroendocrine Aspects of Skin Aging, Int J Mol Sci. 2019; 7:20.
4 Slominski A, et al. Sensing the environment by the skin’s neuroendocrine system. Adv Anat Embryol Cell Biol 2012; 212:1-115.
5 Nordlind K, et al. The skin as a mirror of the soul- exploring the possible roles of serotonin. Exp Dermatol 2007; 17(4):301-11.
6 Slominski A, et al. On the role of skin in the regulation of local and systemic steroidogenic activities. Steroids 2015; 103:72-8.
Optimistic Happy Joyful Cheerful
7 Prescott S, et al. The skin microbiome-impact of modern environments on skin ecology, barrier integrity and immune reprogramming, World Allergy Organ J 2017; 10(1):29.
Figure 8: Clinical evaluation of the positive emotions. PERSONAL CARE ASIA PACIFIC
8 McCabel C, et al. Cognitive influences on the affective representation of touch and the sight of touch in the human brain. Scan 2008; 3:97-108.
November 2020
% Improvement versus placebo Positive emotions
Activated zones
Activated zones
Activated zones
Activated zones
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