68 DEODORANTS 10000000
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10000
C.acnes
S. aureus
P.aeruginosa Figure 2: Analysis of the broad spectrum bacteriostatic and fungistatic effect.
imbalance in the bacterial ecosystem. The active ingredient is rich in anti-quormones (anti-Quorum Sensing molecules) and has been shown to be able to prevent the formation of bacterial communication or coordination factors (Niche Factors) and unwanted bacterial strategies involved in generating the bad smell. This ingredient represents a new activity profile for this species claiming the Plasma Rich in Cell Factors as a new combination of bioactives (metabolome) from the plant Morinda citrifolia.
A prebiotic strategy A specific and innovative prebiotic cocktail based on sugars that shifts the metabolism of commensal microbiota from lipids to polysaccharides.
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C.striatumpopulation in suspension Living cells
Microorganisms detect these polysaccharides as an easier alternative to metabolise the nutritional supply and change their metabolism from lipids to sugars resulting in less odorous by- products.
In vitro efficacy The first strategy of the active (biological activity) is based on its anti-Quorum Sensing (Quorum Quenching) characteristics. A series of in vitro tests were carried out to evaluate the properties of the active that demonstrate the interaction with the microbiota responsible for body odour.
In vitro 1: Analysis of the broad spectrum bacteriostatic and fungistatic effect Firstly, the broad spectrum bacteriostatic
Control DEOBIOME NONI PRCF
and fungistatic effect was demonstrated by quantifying the microbial population in the absence and presence of the active ingredient. It was found that there was significant variation between the untreated cultures and the cultures treated with the active after 24 hours in various species: C. acnes, C. aureus, P. aeruginosa, C. striatum, E. floccosum and M. furfur. The active showed a bacteriostatic effect stopping the growth of the indicated species and maintaining the initial levels but without bactericidal effect (Fig 2).
In vitro 2: Anti-Quorum Sensing effect Secondly, the anti-biofilm effect (the formation of biofilm is one of the first consequences of Quorum Sensing activation) in Corynebacterium striatum, Staphylococcus aureus, Cutibacterium acnes and Malassezia furfur was evaluated by fluorescence staining and Laser Scanning Confocal Microscopy. The active was shown to inhibit the biofilm formation up to 99% while keeping bacterial cells in suspension live (Fig 3).
Inoculum Day 0
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C. striatumControl Control 4.5% NONIPRCF
C.striatumbiofilm population on coupons
In vivo efficacy In order to demonstrate the efficacy of the active, several in vivo tests were performed.
-99% 4.5% NONIPRCF Figure 3: Analysis of anti-biofilm effect on Corynebacterium striatum. PERSONAL CARE GLOBAL August 2020
In vivo 1: Evaluation of armpit odour modulation A first group of 19 volunteers between 20 and 56 years old, applied a placebo cream to one of their armpits and a cream containing 1% of the active to the other armpit to evaluate the armpit odour modulation.
C.striatum
E. floccosum
M.furfur
C.F.U
C.F.U
C.F.U.
Inoculum Day 0 Control (24h) 3% NONI PRCF
Inoculum Day 0 Control (24h) 3% NONI PRCF
Inoculum Day 0 Control (24h) 3% NONI PRCF
Inoculum Day 0 Control (24h) 3% NONI PRCF
Inoculum Day 0 Control (24h) 3% NONI PRCF
Inoculum Day 0 Control (24h) 3% NONI PRCF
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