32 EYE AREA CARE 0 DAYS 56 DAYS 0 DAYS VS. 56 DAYS
2% Myrothamus flabellifolius extract
Placebo
Figure 6: Longest eyelashes before (green line) & after (red line) active and placebo treatments The extract can revive eyelashes by increasing
to produce hair fibres. Myrothamnus flabellifolius extract was found to increase the proliferation of HFDPC by 33% when compared to the control Furthermore, the extract was found to also
improve the proliferative capacity of keratinocytes by 16.1% compared to a control. Therefore, the active ingredient can increase the proliferation capacity of hair follicle dermal papilla cells to promote hair growth and can increase the proliferation capacity of keratinocytes to create stronger hair fibres. Hair anchorage depends upon the adhesion of
different cells to the basement membrane zone, with hemidesmosomes formed by collagen XVII and integrin β4 acting as the main binding units. Immunostaining for collagen XVII showed that there was an increase in collagen XVII after the treatment with the active ingredient, compared to the control. In the upper part of the hair follicle, collagen XVII increased by 143.0% while in the lower parts it increased by 229.7% (Figure 5). In addition, integrin β4 is also a crucial
component of hemidesmosomes. From immunofluorescence staining of the upper and lower follicle, it was determined that treatment with the active ingredient increased integrin β4 by 221.9% and 204.1%, respectively. The results for collagen XVII and integrin
β4 demonstrated that the Myrothamnus flabellifolius extract had a statistically significant effect on boosting these important components of hemidesmosomes along the hair follicle for improved hair fibre anchorage.
0 DAYS
their length and improving lash lift. After 56 days of treatment, the mean length of the longest five eyelashes increased by 3.1% after the treatment, on average, a statistically significant improvement over initial time (p<0.05). This represented a 164% increase in length compared to the placebo. Using ImageJ analysis (Figure 6), a line was
traced following the longest eyelashes before (green line) and after (red line) active and placebo treatments. Then, the two images were superimposed to visualise the differences in eyelash length before and after the treatment. With the active treatment, the red line is placed further out compared to the green line, showing an increase in eyelash length. For the placebo treatment, both lines remain in the same position, indicating there was no difference in eyelash length after the treatment. Furthermore, analysis of lateral
macrophotographs of volunteers demonstrated an average reduction in the curvature angle before and after the treatment, thus indicating a lash-lifting effect. The active treatment helped to decrease the curvature angle by 3.25%, on average, compared to placebo (p<0.01). Myrothamnus flabellifolius extract has a
filling effect for bold and attractive eyebrows. Macrophotographs of the eyebrows of volunteers showed the mean density of eyebrow hair increased by 16.9%, on average. The best result showed an improvement of 86.3% after 56 days, suggesting a
56 DAYS
filling effect for thicker and fuller eyebrows. Using ImageJ analysis (Figure 7), macroscopic
photographs were converted into binary images with the hair coloured in white (before the treatment) and in red (after the treatment). As can be observed, there is more red than white in the images, meaning that there is more hair present after 56 days of treatment than before starting. Throughout the treatment, the volunteers were
asked to log the number of hairs that fell from their eyelashes and eyebrows daily. After 56 days of treatment, the number of eyebrow hairs that were lost was 15.6% lower with the active treatment, compared to hairs lost with the placebo treatment. Similarly, the number of hairs that were lost
from eyelashes treated with the active was 57.6% lower, when compared to the placebo treatment. These results suggest that the Myrothamnus flabellifolius extract not only promotes the growth but also helps minimise the loss of hair in both eyebrows and eyelashes.
Conclusion Resulook™ advanced botanical ingredient proved, in vitro, to induce the proliferation of hair follicle dermal papilla cells and keratinocytes for proper hair growth, while boosting microvasculature and favouring the anchorage of the hair. When applied as a serum and mascara on volunteers, this advanced botanical ingredient helps obtain longer and fuller eyelashes and eyebrows for a more revived and beautiful look.
PC 0 DAYS VS. 56 DAYS
2% Myrothamus flabellifolius extract Figure 7: Binary images with hair coloured in white (before treatment) & red (after) PERSONAL CARE March 2022
www.personalcaremagazine.com
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