70 LONGEVITY A CONTROL 2% MYCONOSIDE-RICH EXTRACT - 24h B 200 50 Control -100
2% Exosome- rich extract
Mitochondrial mass increase for 24h
Figure 6: Fluorescence microscopy of BJ cells after 24-hours treatment with 2% Exosome-rich extract. Mitochondria stained with BioTracker 488; nuclear staining with Hoechst 33342. (A) Representative images showing mitochondrial morphology and distribution. (B) Quantification of mitochondrial mass, shown as a percentage of the control (100%). Adapted from: Georgieva M, Vasileva B, Ivanov P et al. Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells. Cosmetics. 2025;12(3):90. doi:10.3390/cosmetics12030090
A CONTROL 2% MYCONOSIDE-RICH EXTRACT 2% EXOSOME-RICH EXTRACT
CDKN1A (p21) Treatment with 2% Myconoside-rich extract and 2% Exosome-rich extract upregulated CDKN1A (p21) expression by 3.7-fold and 5.8-fold, respectively. The gene is associated with cell cycle regulation and stress response, indicating a protective mechanism against cellular senescence in ageing fibroblasts.2
B Lysosomal mass increase for 72h
100 0
Control
2% Myconoside- rich extract
100 0
Lysosomal mass increase for 72h Control
2% Exosome- rich extract
Figure 7: Light microscopy of lysosomes in BJ cells after 72 hours treatment with 2% Myconoside-rich extract and Exosome-rich extract, stained with Neutral Red. (A) Representative images of lysosomal distribution and structure. (B) Quantification of lysosomal mass, shown as a percentage of control (100%). Adapted from: Georgieva M, Vasileva B, Ivanov P et al. Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells. Cosmetics. 2025;12(3):90. doi:10.3390/cosmetics12030090
Gene expression (time fold)
2% Myconoside- rich extract
2% Myconoside- rich extract
Control 0 1 2 3 4 5 6
Figure 8: RT-qPCR analysis of the effects of 2% Myconoside-rich extract for 24h and 72h on the expression of age-related genes (COL3A1, COL3A3, CDKN1A, and NFE2L2) in BJ cells. Adapted from: Georgieva M, Vasileva B, Ivanov P et al. Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells. Cosmetics. 2025;12(3):90. doi:10.3390/ cosmetics12030090
PERSONAL CARE MAGAZINE September 2026 NFE2L2 ■ CDKN1A ■ COL3A1 ■ COL1A1 ■
NFE2L2 (NRF2) Treatment with 2% Myconoside-rich extract and 2% Exosome-rich extract upregulated NFE2L2 (NRF2) expression by 5.4-fold and 5-fold, respectively. This increase leads to enhanced activation of protective genes against oxidative stress, which is crucial for maintaining skin health and mitigating signs of ageing.2
Conclusion As skin ages, cellular energy production diminishes, leading to a visible loss of vitality and a reduction in active, functional cells. This age- related decline is closely linked to mitochondrial dysfunction, which compromises the skin’s ability to regenerate, repair and maintain a youthful appearance.
The findings of this study demonstrate
the remarkable potential of H. rhodopensis in vitro culture extracts in next-generation skin care, positioning InnovaBioTech Haberlea and InnovaBioTech Resurrection as high-performance active ingredients for advanced dermocosmetic formulations. Scientifically proven to enhance skin integrity, elasticity and resilience, these bioactive botanicals deliver visible improvements in skin vitality, tone and luminosity—promoting a rejuvenated, age-defying complexion. Aligned with the growing industry focus on
longevity science, cellular rejuvenation and pro-youth innovation, these biotech ingredients activate key mechanisms linked to skin resilience and regeneration. This makes them ideal for premium anti-ageing lines, including prevention- oriented routines, skin reset therapies and epigenetic beauty solutions that support the skin’s natural self-repair and defence systems. Moreover, as clean beauty and sustainability
remain top priorities for consumers, these actives meet the demand for plant-based, eco- responsible, and clinically validated ingredients. They empower brands to formulate with
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