SKIN CARE 43 n=4, **p<0.01. *p<0.05. 300 ** ** 200 1 100 0.5 0 UVB VC-3G UVA (mmoI/L) 1 10 Blue light (mmoI/L) 1 10 Near-infrared (mmoI/L) 1 10
Figure 13: Evaluation of suppressing effect of ROS using normal human skin fibroblast
in fresh medium for one additional day. The culture supernatant was collected and applied to normal human dermal fibroblasts (NHDF), and the type I collagen production was quantified by using an ELISA assay. As shown in Figure 12, VC-3G suppressed the UVB-induced reduction in collagen synthesis in a dose-dependent manner.
Against UVA, blue light and near-infrared radiation (NIR) To evaluate the anti-photoageing effects of VC-3G against UVA, blue light, and near-infrared radiation (NIR), we examined its inhibitory effects on ROS production and elastin expression reduction caused by these types of radiation. For the evaluation of ROS production inhibition,
NHDF treated with VC-3G were incubated with an ROS-reactive fluorescent probe and exposed to UVA (10 J/cm2 (200 J/cm2
), blue light (100 J/cm2 ), or NIR ). The fluorescence intensity was then
measured to quantify ROS levels. As shown in Figure 13, VC-3G suppressed the
increase in intracellular ROS production induced by UVA, blue light, and NIR in a dose-dependent manner. For the evaluation of its effect on elastin
expression, NHDF treated with VC-3G were exposed to UVA (10 J/cm2 or NIR (200 J/cm2
), blue light (100 J/cm2 ), ). Cells were collected 24 hours after exposure, and the mRNA relative expression Before After 8 weeks
120 100 80 60 40 20 0
More
Conclusion VC-3G, introduced in this study, represents a groundbreaking new ascorbic acid derivative that effectively addresses the key challenges of stability and formulation associated with ascorbic acid and its derivatives. Beyond its well-known roles in increasing
collagen production as well as reducing melanin, VC-3G exhibits antioxidant properties, one of the essential physiological functions of ascorbic acid. Although ascorbic acid possesses powerful
0 Light VC-3G UVA (mmoI/L) 0.3 Blue light (mmoI/L) 0.3
Near- infrared
(mmoI/L) 0.3
Figure 14: Evaluation of suppressing effect of elastin reduction using normal human skin fibroblast (measurement of mRNA expression level)
level of tropoelastin, a key component of elastic fibres, was quantified by real-time PCR. As shown in Figure 14, VC-3G suppressed the reduction in elastin expression induced by UVA, blue light, and NIR in a dose-dependent manner.
Clinical study of anti-photoageing effects To evaluate the anti-photoageing effects of VC-3G in humans, we assessed its efficacy in wrinkle reduction. A 2% aqueous solution of VC-3G was applied twice daily for 8 weeks, and wrinkle scores were measured before and after the treatment using VISIA Evolution (Canfield Scientific Inc.). As shown in Figure 15, continuous application
of VC-3G resulted in a reduction in wrinkle- recognition areas and a decrease in wrinkle scores, demonstrating that VC-3G effectively improved wrinkles.
antioxidant properties that help eliminate ROS, its efficacy is often compromised by issues related to stability and formulation. In contrast, VC-3G offers superior stability
and can be incorporated into a wide range of stable formulations, making it highly suitable for daily skin care products aimed at preventing ageing. VC-3G also effectively inhibits protein carbonylation. Since carbonylated proteins have been reported to be associated with skin clarity, VC-3G is expected to contribute positively to this regard as well. In recent years, photoageing has become
a growing concern. Studies have shown that photoageing is not only caused by UV radiation but also by blue light and near-infrared light. Exposure to these rays induces the production of reactive oxygen species (ROS) in the skin, leading to collagen and elastin degradation, wrinkles and sagging. As a result, it is possible to use VC-3G in daily care cosmetics to prevent photoageing. By comprehensively addressing various skin
concerns, SEIWA KASEI’s novel ascorbic acid derivative VC-3G, product name iVC™ 3GA (INCI: 3-Glyceryl Ascorbate), contributes to a holistic approach in advancing overall skin well-being. We are committed to advancing VC-3G as
a next-generation, stable, and user-friendly ascorbic acid derivative. In addition, we have developed a range of ascorbic acid derivatives under the iVC Series. Each product in the iVC Series boasts exceptional formulation stability and multifunctionality, overcoming the conventional limitations of ascorbic acid and unlocking new possibilities for skin care innovation across diverse cosmetic applications.
PCM Less Before 8 weeks After
References 1. Correia G, Magina S. Efficacy of topical vitamin c in melasma and photoaging: a systematic review. Journal of Cosmetic Dermatology. 2023; 22(7), 1938-1945
2. Telang PS. Vitamin C in dermatology. Indian Dermatology Online Journal. 2013; 4(2), 143-146
Figure 15: Evaluation of anti-wrinkle effect by VISIA (in vivo test)
www.personalcaremagazine.com September 2026 PERSONAL CARE MAGAZINE ** ** * ** ** ** 1.5 ** ** * ** * n=3, **p<0.01. *p<0.05. 2 **
Intracellular ROS (% of control)
Change ratio of wrinkles (%)
Relative expression levels of tropoelastin mRNA
Amount of wrinkle
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