68 LONGEVITY dn=114 nm ■ di 30 25 20 15 10 5 0 10 100 Diameter (nm)


Figure 1: DLS was used to analyse particle size distribution within the EV fraction isolated from the conditioned medium of H. rhodopensis in vitro cultures. The particle diameters reflect the primary peaks based on number (dn), volume (dv) and intensity (di)2


A B C 1000 10000 =380 nm ■ dv =400 nm ■


toxicity such as cytoplasmic granulation, vacuole formation, or nuclear irregularities, indicating preserved cellular health and integrity. This indicates good biocompatibility, supporting their strong prospects for safe, skin-rejuvenating cosmetic and dermatological applications.2


Supporting the cytoskeletal organization and cellular plasticity, ensuring proper cell structure and resilience The actin cytoskeleton — the network inside cells that controls their shape, movement and repair — is crucial for healthy cell function, shape and skin integrity. To assess the impact of the extracts on the cytoskeleton, BJ fibroblasts were treated with 2% Myconoside-rich extract and 2% Exosome-rich extract for 24 hours. After treatment, cells were stained with a


fluorescent dye that specifically binds to F-actin. Fluorescence microscopy was then used to visualize the organization and distribution of actin filaments within the cells. Results showed no changes or disruptions in the treated cells, indicating that their actin filaments remained well-organized, supporting cellular structure and stability.2


Enhancing mitochondrial function, energy levels and cellular repair capacity Mitochondria — often called the “powerhouses” of the cell — are essential for producing the energy needed for all cellular activities, including growth, repair and regeneration.7 BJ cells were treated with 2% Myconoside-


D E


rich extract and 2% Exosome-rich extract for 24 and 72 hours. After treatment, cells underwent dual staining using Biotracker 488 to assess mitochondrial integrity and Hoechst 33342 for nuclear staining. Fluorescent images were captured using epi-fluorescence microscopy and analysed quantitatively with ImageJ software.2 Treatment with 2% Myconoside-rich extract


Figure 2: TEM images of extracellular vesicles from H. rhodopensis in vitro cultures show individual vesicles ranging from 50 to 200 nm in size. The vesicles display mostly round shapes, with occasional oval forms and clearly defined lipid bilayer membranes2


CONTROL 2% MYCONOSIDE-RICH EXTRACT


for 24 and 72 hours resulted in an increase in mitochondrial mass by approximately 1.08-fold and 1.31-fold, respectively, compared to untreated cells. This boost in mitochondrial mass enhances cellular energy levels, equipping the cells with the necessary resources for repair and regeneration. The gradual improvement observed suggests a cumulative effect, highlighting the long-term ingredient’s potential in anti-ageing skin treatments.2


2% EXOSOME-RICH EXTRACT


Figure 3: Phase-contrast microscopy of BJ cell morphology after treatment for 24 hours with 2% Myconoside-rich extract and 2% Exosome-rich extract. Adapted from: Georgieva M, Vasileva B, Ivanov P et al. Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells. Cosmetics. 2025;12(3):90


PERSONAL CARE MAGAZINE September 2026 www.personalcaremagazine.com


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