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FRAGRANCE 59


defined as the sum of “Agree” and “Strongly agree”, and 23 questionnaires were analysed at day 56. When collected using a rigorous and appropriate protocol, consumer data provide scientifically valid support for the assessment of consumer-perceivable attributes (Figure 4). The following outcomes are reported using


standardised perception-based statements. First of all, 96% notice the perfume is pleasant.


This high level of agreement indicates the absence of any noticeable unpleasant off‑odour, including algae-like notes that could negatively impact fragrance perception. Regarding skin hydration and barrier-related


properties, consumer perceptions indicate that 87% of participants noticed softer skin, while 74% reported moisturised skin and 70% perceived increased skin suppleness. In addition, 83% of consumers indicated that the skin was protected from external aggressions. Regarding skin comfort and reactivity, 87%


of participants reported a soothing effect, and 74% noticed reduced visible redness. These consumer-reported outcomes suggest a decrease in perceived skin discomfort and signs associated with cutaneous reactivity. In terms of skin surface characteristics, 83%


of consumers noticed smoother skin, while 74% reported a reduction in the appearance of wrinkles or fine lines. These results indicate a positive perception of skin texture and micro-relief. About the maintenance or improvement of skin


mechanical properties, 83% of participants noticed preserved skin elasticity, while 74% reported improved skin firmness and 70% perceived firmer skin. These results reflect a consistent consumer perception related to skin biomechanical quality. These perceptions show coherence with


instrumental cutometry measurements assessing elasticity parameters, and with in vitro tests demonstrating inhibition of collagen and elastin degradation. Together, these data support a mechanistic alignment between biological activity, objective measurements, and consumer-reported outcomes related to skin mechanical properties. Concerning skin radiance and visual


complexion parameters, 78% of consumers noticed improved skin radiance, while 74% perceived a more unified complexion and 70% reported a more homogeneous complexion. These outcomes reflect a positive evolution of overall skin appearance from the consumer perspective. These consumer perceptions are coherent with in vitro results demonstrating inhibition of tyrosinase activity, a key enzyme involved in melanogenesis, which supports a biological mechanism related to pigmentation regulation. In addition, they are consistent with


clinical assessments performed using VISIA-7 imaging, which showed a statistically significant reduction in superficial dark spots. Together, the convergence of tyrosinase inhibition in vitro, objective VISIA-7 clinical data, and consumer-reported improvements in radiance and complexion homogeneity, reinforces the internal consistency between biological activity, instrumental measurements, and visual consumer perception, without implying direct equivalence


www.personalcaremagazine.com 83%


Revitalised skin


83% Smoother skin


100% 80% 60% 40% 20% 0%


74%


Reduced wrinkles / fine lines


78%


Improved skin radiance


83%


Preserved elasticity


74% Improved firmness


Figure 4: Consumer self-assessment at Day 56 showing the proportion of favorable responses for selected skin-related attributes following product use


between these different evaluation levels. Finally, 83% of participants reported


revitalised skin, reflecting a global improvement in perceived skin quality. This holistic perception likely integrates multiple dimensions, including hydration, comfort, texture, mechanical properties and radiance.


Conclusion This work demonstrates that a fragrance concentrate can be engineered to function as a carrier for a marine-derived bioactive system while maintaining compatibility with perfumery constraints. Internal in vitro dose–response data show preserved antioxidant and enzyme inhibition activities following integration into fragrance matrices at low concentration ranges. Independent finished‑product studies provide


instrumental evidence for significant improvement of superficial spot–related endpoints and parameter‑specific modulation of skin elasticity, together with high consumer-reported acceptability after 56 days of use. Taken together, these findings support the


feasibility of a bioactive fragrance approach as a multifunctional formulation strategy.


References 1. Cosmetics Europe. Guidelines for Cosmetic Product Claim Substantiation. Brussels; 2019. https://cosmeticseurope.eu/wp-content/ uploads/2024/10/Guidelines_for_Cosmetic_ Product_Claim_Substantiation.pdf


PCM


2. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–1237


3. Pagels F et al. Evaluation of antioxidant capacity using the ABTS radical cation decolorization assay. [Journal title]; 2022


4. Adhikari B, Dhungana SK, Imran U et al. Inhibition of mushroom tyrosinase by plant extracts: L-DOPA oxidation assay. J Nat Prod. 2008;71(5):815–818


5. MilliporeSigma. Enzymatic Assay of Collagenase (EC 3.4.24.3) Using FALGPA. Technical protocol. https://www.sigmaaldrich. com/US/en/technical-documents/protocol/ protein-biology/enzyme-activity-assays/ enzymatic-assay-of-collagenase-using-n-3- 2furylacryloyl-leu-gly-pro-ala


6. Tribioscience. Suc-Ala-Ala-Ala-p-nitroanilide: chromogenic substrate for elastase assays. Technical datasheet. 2024. https://tribioscience. com/wp-content/uploads/2024/12/TBP0108- Suc-Ala-Ala-Ala-pNA_2024.pdf


7. Laboratoire COSMEPAR. Study 2308516. Instrumental imaging (VISIA-7). Final report. Nantes; 2026


8. Laboratoire COSMEPAR. Study 2308515. Cutometry – firmness and elasticity. Final report. Nantes; 2026


9. Laboratoire COSMEPAR. Study 2308517. Consumer self-assessment test. Final report. Nantes; 2026


September 2026 PERSONAL CARE MAGAZINE


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