60 EYE AREA CARE T0 DAYS T7 DAYS DARK CIRCLES (%) T7 days T14 days T28 days
-12%**
-18%*** iPeptide Figure 2: Efficacy on dark circles.
Providing melatonin-like protection One of the most well-known regulators of circadian rhythms is melatonin. Once it binds to its receptor, it regulates sleep, immune and inflammation responses. On the other hand, thanks to its chemical nature, melatonin also acts as a potent antioxidant, able to protect proteins from glycation. In a study, the expression of the gene of the
melatonin receptor 1a was increased by 2.1 times in the presence of Acetyl Hexapeptide-8, while in another study AGEs were reduced by +19% compared to melatonin and anti-peroxidation activity was comparable to melatonin.
Restoring lymphatic integrity The lymphatic system is part of the immune system, which clears the body of waste and inflammatory substances. In order to study the restorative impact of
Acetyl Hexapeptide-8 on the lymphatic system, two targets were identified. Firstly, emilin-1 is an ECM glycoprotein highly expressed in LECs (lymphatic endothelial cells). They have an important role in anchoring filaments and lymphatic vessel formation. Secondly, integrin- a9β1 is preferentially expressed in lymphatic endothelial cells and is also involved in
lymphatic vessel formation and function. In the study it was shown that 0.05 mg/
mL of Acetyl Hexapeptide-8 increased emilin-1 production by 71% vs basal. In addition, integrin-a9β1 production was increased by 54%.
Capillary de-stressing Sleep deprivation leads to an exaggerated immune response (alarmin IL-33), which results in the formation of blood capillary meshes, increasing the level of vascular stress. To study the impact of Acetyl Hexapeptide-8
on the formation of excessive capillary meshes, the number of meshes induced by IL-33 were counted in human umbilical endothelial cells with and without the peptide. The results showed that presence of Acetyl Hexapeptide-8 reduced the number of meshes to basal levels.
Improving hyperpigmentation IL-33 also plays a significant role in the development of hyperpigmentation, resulting in dark circles as it has a pro-melanogenic effect due to the increase in tyrosinase activity and melanin content. The level of tyrosinase activity was measured to assess the impact of Acetyl Hexapeptide-8. It was measured against
HYPERPIGMENTATION FADING (TYROSINASE ACTIVITY, %) 100% 86%* 73%*** 78%**
kojic acid – an industry benchmark for treating hyperpigmentation. The results showed that kojic acid was able to fade hyperpigmentation by 27%, while 0.5 mg/mL of Acetyl Hexapeptide-8 was nearly able to match the industry benchmark with a result of 22% (Fig 3).
Self-assessment Consumers are increasingly demanding results they can clearly perceive themselves. To establish this, LipoTrue conducted a volunteer self-assessment study involving 22 men aged 34-50 years old, sleeping 5-6 hours/day and 24 women aged 50-64 years old sleeping 5-6 hours/day. They each applied a cream containing 3% Acetyl Hexapeptide-8 twice a day for 28 days. The results showed that: ■ 43% said their look is more open ■ 73% said they feel like they slept all night ■ 83% said they feel their skin is more refreshed ■ 81% said their skin is smoother and more moisturised ■ 57% said they look less tired ■ 69% said it had an anti-fatigue effect ■ 63% said it had a cooling effect
Non treated
0.06 mg/mL kojic acid
Figure 3: Efficacy on hyperpigmentation. PERSONAL CARE May 2021
0.25 mg/mL iPeptide
0.5 mg/mL iPeptide
Conclusion iPeptide has been proven to attenuate inflammation, prevent damage caused by high levels of glucose, restore the skin immune capacity and provide a melatonin- like protection. It has also showed its ability to improve hyperpigmentation and reinforce epidermal barrier and endothelial and lymphatic vessels, helping the skin to be more prepared to defend itself. The in vivo results confirmed what had been observed before. The peptide smoothed fatigue signs, opened the look, decongested eyebags, faded dark circles and provided a local cooling effect. iPeptide, from LipoTrue, can be considered an indispensable ingredient for cosmetics aimed at people who regularly skip sleep, which in these pandemic times is most of the population. It is time to start biohacking sleep with iPeptide to fight the signs of fatigue (69% of volunteers think so).
-19%***
www.personalcaremagazine.com PC
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