40 HYGIENE 10000000 1000000 100000 10000
M. furfur
inflammatory system of the skin, causing the appearance of skin imperfections. This metabolome is rich in Anti-Quorum
Sensing molecules (anti-Quormones), especially designed for the ‘dermohacking’: they act synergistically against the microbial dysbiosis mediated by Quorum Sensing and bring the activity previously described. The plant (origin of the active) is widely used
in traditional Polynesian medicine and has its origin in the islands of the South Pacific such us Tahiti and Fiji, with more than 150 actives described. Noni plant is used worldwide for analgesic, anti-tumour, and anti-inflammatory remedies. There are numerous clinical studies that validate the remarkable benefits of the plant for our health. The mechanism of action of the active
(Quorum Quenching – Fig 1) is based on the natural strategy of Noni and consists of blocking the bacterial coordination (QS), respecting the skin microbiota and being an alternative to the use of antibiotics. The composition of the active is 100%
C. acnes Figure 2: Bacteriostatic/fungistatic effect of Quora Noni.
natural (ISO 16128), preservative free, certified by COSMOS-Ecocert and respectful to the microbiota.
Biological activity In vitro 1: Broad spectrum bacteriostatic effect Several microorganism’s species were incubated (M. furfur, C. acnes, S. aureus, P. aeruginosa, C. striatum and E. floccosum) with several concentrations of the active during 24h, under specific conditions for each microorganism. Viable cells were quantified at time 0 and after 24h of contact to evaluate the bacteriostatic and fungistatic activity of the active. The result shows that the active ingredient
is able to inhibit the growth and reproduction of several microorganisms (G+, G- and Fungi), maintaining the populations without destroying the microbes, demonstrating, therefore, its bacteriostatic effect (Fig 2).
In vitro 2: Anti-biofilm effect (anti-Quorum Sensing) In this second assay, the anti-biofilm properties
of the active were evaluated and representative biofilms were generated in C. acnes, M. furfur, C. striatum and S. aureus. The objective of the trial was the quantification of: ■ Biofilm population density, measured as colony-forming units per coupon, after 24h of incubation. ■ Planktonic live cells in suspension It was demonstrated that the active significantly inhibited the biofilm formation up to 99% while preserving the planktonic cells alive, showing its antibiofilm effect on Cutibacterium acnes and Staphylococcus aureus (Fig 3 and 4, respectively).
In vitro 3: Modulation of microbial quormones synthesis In order to measure the ability of the ingredient to inhibit the expression of Lux-S Gene, a key gene in the synthesis of Quormones, an experiment was specially designed: Gene expression was measured through Retro- transcription and quantitative PCR, expressing the resulting values in Cqs.
S. aureus
P. aeruginosa
C. striatum
E. floccosum
Figure 3: Anti-biofilm effect in C. acnes. PERSONAL CARE May 2021
Figure 4: Anti-biofilm effect in S. aureus.
www.personalcaremagazine.com
C.F.U.
Inoclum Day 0 Control
5% QUORA NONIPRCF 10% QUORA NONIPRCF
Inoclum Day 0 Control
5% QUORA NONIPRCF 10% QUORA NONIPRCF
Inoclum Day 0 Control
5% QUORA NONIPRCF 10% QUORA NONIPRCF
Inoclum Day 0 Control
5% QUORA NONIPRCF 10% QUORA NONIPRCF
Inoclum Day 0 Control
15% QUORA NONIPRCF
Inoclum Day 0 Control
30% QUORA
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