HYGIENE Δ MICROBIOTA POPULATION AFTER 30 DAYS TREATMENT 1% QUORA NONI COMPARED TO PLACEBO
S.aureus S.epidermidis
C.acnes -60 -50 -40 -30 *p<0.05 Figure 5: Dysbiosis rebalance and Microbial quantification.
The results show that the active was able to inhibit the Lux-S Gen expression up to 89%. This means that the active has the ability to interfere very specifically in the Quorum Sensing system of C. acnes, by inhibiting the synthesis of Quormones, and offering a new mechanism of action.
Siltech 2ChAd 21.qxp_Layout 1 07/04/2021 16:52 Page 1
In vitro 4: Modulation of Toll-like Receptor-2 (TLR2) This test measured the ability of the active to block the membrane receptors TLR2 (microbe- keratinocyte connection), the ability of the activation of TLR2 of a solution of C. acnes lysate was measured. The TLR2 activation by fragments of C. acnes membrane leads to IL-α
induction, which is, therefore, a marker of this interaction. The keratinocytes were incubated during
24h with the ingredient, and the supernatant was then eliminated and cleaned, and the pre- treated keratinocytes were exposed to the C. acnes. IL-1α was measured by ELISA. The active was able to decrease the levels of
IL1-α up to -46% compared to control without treatment. This means that the ingredient has the ability to interfere very specifically in the microbial-keratinocyte interaction by blocking the Toll-like Receptor-2.
In vitro 5: Anti-inflammatory activity To measure the anti-inflammatory activity of the
ingredient, Human Inflamed Monocytes (THP1) were treated with the active for 24h, and then the levels of TNF-α and IL-8 were quantified from supernatant by ELISA. The positive control was dexamethasone (reference drug). The active completely restored TNF-α
levels from inflamed cells. The effect was very potent at all tested doses, reaching up to -97%, demonstrating a higher level of restoration than dexamethasone (-56%). The ingredient also completely restored IL-8
levels from inflamed cells. The effect was very potent at all tested doses reaching up to -69%. Dexamethasone restoration levels was up to -33%. This demonstrates the anti-inflammatory ability of the ingredient, even higher than dexamethasone.
-20 -10 0 -54%
Staphylococcus epidermidis
1.2% Drybiosis -50% 10 Skin
Cutibacterium acnes
Staphylococcus aureus
41
www.personalcaremagazine.com
May 2021 PERSONAL CARE
Relative proportion
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