SUN CARE A significant reduction in ROS production
was observed in cells treated with Vigna radiata vacuolar extract compared to the negative control. Approximately 60% reduction in ROS levels was measured across all tested product concentrations (Figure 1), demonstrating that Vigna radiata vacuolar extract provides a protective effect against blue light by significantly reducing oxidative stress in human keratinocytes.6
Protective action against IR Based on the results of the MTT assay, a 1.00% concentration of Vigna radiata vacuolar extract was selected. Human keratinocytes were treated with 1% of the extract and then irradiated with infrared (IR) radiation at a wavelength of 800 nm, which is known to cause significant cellular damage. The results demonstrated that ROS
production in cells exposed to IR was significantly lower in the presence of Vigna radiata vacuolar extract with a reduction of around 18% (Figure 2). This indicates that the vacuolar extract provides a protective effect, reducing oxidative stress in keratinocytes exposed to IR radiation.7
In vivo evaluation of the soothing effect on UV-induced skin erythema This test aimed to evaluate the protective action of Vigna radiata vacuolar extract against UV-induced damage by measuring its ability to soothe skin redness. The erythema index
Untreated skin ■ Placebo ■ Plant C - Stem Vigna radiata ■
120 100 80 60 40 20 0
*p< 0.05 vs baseline ** p<0.01 vs Placebo *** p>0.05 vs baseline
***
20% **
*
Figure 3: Evaluation of UV-induced erythema after a single application of placebo vs. a formulation containing Vigna radiata vacuolar extract
was measured one hour after applying the product on the volunteers’ back, following UVA + UVB exposure, and compared with both an untreated control area and an area treated with a placebo. The panel test was conducted under the supervision of a dermatologist on 20 Caucasian women over the age of 50, with phototypes 2 and 3 and no skin pathologies. After the product was applied to the
volunteers’ backs, they remained in the laboratory for two hours under controlled humidity and temperature conditions. The test
began with the determination of the minimal erythema dose (MED), which is defined as the threshold dose of solar radiation that causes sunburn. The subjects were irradiated on their backs using a solar simulator with six tailored doses of solar radiation. Approximately 20 ± 4 hours after irradiation, the MED was visually assessed by the investigator. Following this, the induction of damage was
carried out by irradiating three skin sites on each subject with a fixed dose of UV radiation (1.5 MED). This was done to induce a reaction
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www.personalcaremagazine.com
June 2025 PERSONAL CARE
Variation in UV-induced erythema (%) vs baseline
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