SKIN MICROBIOME 67 TNF-α (pg/mL)
10 8 6 4 2 0
CTR- CTR+ -32.6% * 1%
EquiBiotics LA + SLS
Figure 1: Anti-inflammatory activity: TNF-α expression in ex vivo test, ***p-value<0.001; **p-value<0.01; *p-value<0.05
an impaired barrier function of stratum corneum in sensitive skin facilitates to the penetration of irritants, resulting in marked cutaneous responses to otherwise harmless stimuli.11 It is precisely in this delicate circumstance
that Roelmi HPC introduces its own postbiotic cosmetic active ingredient. Ex vivo study on reconstructed human
epidermis EpiDerm™ with ELISA method, has demonstrated its efficacy in reducing inflammation, particularly crucial for reactive skin. Following inflammation induced by irritant
agent SLS, there was a notable decrease of 32.6% in TNF-α levels compared to the CTRL+ after 24 hours (Figure 1). The choice of this specific factor is due to its
nature. Tumour Necrosis Factor-alpha (TNF-α) is a protein, classified as a cytokine, that acts as a signaling molecule in the immune system. It plays a crucial role in regulating various biological processes, including inflammation, cell proliferation, and cell death (apoptosis). Although essential for a healthy immune
response, its excessive or chronic production is linked to numerous inflammatory and autoimmune diseases. Its expression is therefore directly related to the product’s anti-inflammatory activity. A second ex vivo study on reconstructed
human epidermis EpiDerm with TEWL method demonstrates is capability to prevent water loss, thus reducing the risk of dryness (after 24 hours),
50 40 30 20 10 0
CTR- CTR+ TEWL -15.9% *
1%
EquiBiotics LA + SLS
Figure 2: Integrity of the epithelial barrier: TEWL in ex vivo test, ***p-value<0.001; **p-value<0.01; *p-value<0.05
another very common fact in sensitive skin. To complete the tests on the anti-inflammatory
activity, a metagenomic analysis of the 16S rRNA gene was carried out always on reconstructed human epidermis EpiDerm. It was observed to effectively restore equilibrium after an oxidative stress induced by 30 µl H2
O2 . The results show a decrease of
Staphylococcus aureus and an increase in Lactobacillus abundance (Figure 3). The increased microbiological biodiversity
observed after applying the product is primarily due to the increase in Lactobacillus spp., an endogenous inhabitant of healthy skin. Therefore, L. acidophilus PBS067 preserves the overall structure of the skin microbiome, but some notable changes in the abundance of less common members have been observed. Paracoccus is recognized as a skin
symbiotic bacterium, representing the phylum Pseudomonas, while Acinetobacter species have demonstrated a protective effect against inflammation in the skin ecosystem. 1% of this specific postbiotic therefore appears to rebalance their abundances to values closer to baseline.
In vivo tests Baby skin is extremely delicate and sensitive. The dermis and epidermis of newborns are structurally and functionally immature compared to that of adults. All newborn skin is approximately 40-60% thinner than that of adults. The same is true for the
epidermis, which is approximately 20% thinner. Another important difference concerns
collagen fibres, which are also smaller than those of adults. However, the dermal-epidermal junction (DER) is similarly less mature. The anchoring structures between the dermis and epidermis, such as anchoring fibrils, are reduced, with large spaces between the connection points. These structural immaturities contribute to
poor barrier function, making newborns more susceptible to increased transepidermal water loss (TEWL) and a greater risk of irritation. A clinical trial on 22 children between
the ages of 3 and 36 months (Figure 4) has been performed. Treatment and evaluation were directed and supported by an expert dermatologist over a period of zero, three and seven days. The tested formula incorporates 1% of
postbiotics and was evaluated on skin erythema. The results at T7 demonstrated a significant action p-value<0.001, allowing a reduction in the score scale from approximately 2.5, therefore moderate, to approximately 1.5, therefore mild. The erythema was reduced in 77.3% of subjects after seven days. To support these data, a photographic
evaluation (Figure 5) with a digital reflex was carried out on the same subjects at T0, T3, and T7. The images reported below clearly show a reduction in redness on the cheeks of the children photographed already at T3. To complete the test, a parental questionnaire
Microbiome composition at T48h - C - vs. C+ vs. EquiBiotics LA
CTR+ CTR-
0% 10% 20% 30%
■ Cutibacterium ■ Unclassified_Actinobacteria ■ Unclassified_ Betaproteobacteria ■ Straphylococcus
40% 50%
■ Corynebacterium ■ Sphingomonas ■ Pseudomonas ■ Unclassified_Caryophanales
Figure 3: Rebalance of skin microbiota: Microbiome composition at T48h C- vs. C+ vs. Equibiotics LA
www.personalcaremagazine.com March 2026 PERSONAL CARE MAGAZINE 60% 70% 80%
■ Micrococcus ■ Unclassified_Neisse ■ Moraxella ■ Lactobacillus
TBF-α pg/mL
TEWL (Ω)
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