LONGEVITY CONTROL STRESS (UV-A) 0.001% CSHR EXTRACT + STRESS
97
P16INK4a■ Nuclear labelling (DAPI) ■ Figure 8: In situ visualization of TNF-α levels. The white dotted line separates the epidermis and the dermis
this decline, demonstrating a 95% efficacy and indicating its potential to protect epidermal integrity and promote renewal.
TNF-α and IL-6 levels When compared to the control, exposure to UV-A stress resulted in a significant elevation in the levels of the inflammatory biomarkers TNF-α and IL-6, as illustrated in Figures 8 and 9, respectively. The CSHR extract effectively counteracted this stress-induced increase, demonstrating a 95% efficacy against TNF-α and a 46% efficacy against IL-6, thereby suggesting its anti-inflammaging properties.
Conclusion Today, the growing emphasis on longevity has intensified the demand for natural and sustainable skin care solutions. Among the most promising candidates are hemp-derived compounds, particularly cannabisins, which exhibit potent antioxidant, anti-inflammatory, and neuroprotective properties. This study investigated the potential of a cannabisin-rich extract derived from bioreactor-cultivated Cannabis sativa hairy roots to enhance skin longevity by activating essential biological pathways, such as proteostasis, autophagy, and protection against cellular senescence. The extraction process utilized a microwave-
assisted technique, which increased the cannabisin F content by 46-fold. When combined with the 100-fold increase in
CONTROL
cannabisin production achieved through hairy root technology, this method enables the recovery of nearly 4,600 times more cannabisin F in the CSHR microwave-assisted extract compared to conventional hemp seed extracts. Ex vivo studies conducted on human
skin samples at concentrations of 0.001%, 0.003%, and 0.01% demonstrated remarkable protective effects against UV-A-induced protein carbonylation. Additionally, the study revealed significant improvements in key biomarkers, including Sirtuin-1, P16INK4A
-positive cells,
LAMP2A, Keratin-14, TNF-α, and IL-6, with efficacy even surpassing that of resveratrol in some cases.
These findings highlight the potential of this
cannabisin-rich eco-extract from hemp hairy roots as a promising and sustainable solution for promoting skin longevity, while ensuring respect for the planet and biodiversity.
PC
References 1. Wang S, Luo Q, Fan P. Cannabisin F from Hemp (Cannabis sativa) Seed Suppresses Lipopolysaccharide-Induced Inflammatory Responses in BV2 Microglia as SIRT1 Modulator. International Journal of Molecular Sciences. 2019; vol. 20 (13), p. 507
2. Kaminsky N, Hubert J, Guerin C, Mazlani M, Kotland A, Pozzobon V, Marant B, Mailhac H, Poigny S. Deciphering the Phytochemical Potential of Hemp Hairy Roots: A Promising Source of Cannabisins and Triterpenes as
STRESS (UV-A)
Bioactive Compounds. Molecules. 2024; vol. 29
3. Vidović T, Ewald CY. Longevity-Promoting Pathways and Transcription Factors Respond to and Control Extracellular Matrix Dynamics During Aging and Disease. Frontiers in Ageing. 2022; vol. 3
4. Baraibar MA, Ladouce R, Friguet B. Proteomic quantification and identification of carbonylated proteins upon oxidative stress and during cellular aging. Journal of Proteomics. 2013; vol. 92, pp. 63-70
5. Moreno-Blas D, Adell T, González-Estévez C. Autophagy in Tissue Repair and Regeneration. Cells. 2025; vol. 14, p. 282, 2025
6. Salminen A, Kaarniranta K, Kauppinen A. Crosstalk between Oxidative Stress and SIRT1: Impact on the Aging Process. Int J Mol Sci. 2013; Vol. 1 (214), pp. 3834-3859
7. Waaijer M, Parish W, Strongitharm B, van Heemst D, Slagboom P, de Craen A, Sedivy J, Westendorp R, Gunn D, Maier A. The number of p16INK4a
8. Alam H, Sehgal L, Kundu S, Dalal S, V. MM. Novel function of keratins 5 and 14 in proliferation and differentiation of stratified epithelial cells. Mol Biol Cell. 2011; vol. 22 (12)
9 Tylutka A, Walas Ł, Zembron-Lacny A. Level of IL-6, TNF, and IL-1β and age-related diseases: a systematic review and meta-analysis. Front Immunol. 2024; vol. 1 (115)
0.001% CSHR EXTRACT + STRESS positive cells in human skin reflects
biological age. Aging Cell. 2012; vol. 11(14), pp. 722-725, 2012
IL-6 ■ Nuclear labelling (DAPI) ■ Figure 9: In situ visualization of IL-1A levels. The white dotted line separates the epidermis and the dermis
www.personalcaremagazine.com November 2025 PERSONAL CARE
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