LONGEVITY
Boosting longevity with hemp-derived extract
Javier Arrieta-Escobar – Inabata France Héloïse Mailhac, Naomi Kaminsky – STH Biotech Kalvin Andrieux – Antofénol
In today’s world, where the pursuit of longevity is increasingly prioritized, the development of innovative, natural active ingredients is crucial to address the rising demand for enhanced quality of life. This shift is further amplified by a growing consumer preference for natural and sustainable skin care solutions, driving research into the potential of hemp-derived compounds for promoting skin health and longevity. Among these compounds, Cannabisin F—a
bioactive molecule primarily found in hemp (Cannabis sativa) seeds and fruits—stands out for its remarkable antioxidant, anti-inflammatory, and neuroprotective properties.1 Recent advancements in biotechnology
have enabled a groundbreaking approach: the amplified production of this molecule using hemp hairy roots in a controlled bioreactor system. This method has achieved a hundred-fold increase in Cannabisin F yield while requiring 74 times less space and 91 times less water compared to traditional hemp seed cultivation.2 Here, we investigate the potential of a
cannabisin-rich extract, derived from bioreactor- grown Cannabis sativa hairy roots (CSHR), to activate key biological pathways linked to skin cell longevity. These pathways include the preservation of proteostasis, the stimulation of autophagy, and protection against cellular senescence.3 The process began with a microwave-
assisted extraction of the CSHR biomass, which was then compared to a conventional extraction method without microwaves. The resulting dried extract was subsequently applied to human skin explants, and its efficacy was evaluated based on the following. ■ Protein carbonylation levels in skin sections, associated with oxidative damage in each skin compartment4 ■ LAMP2A levels, related to chaperone-mediated autophagy, detoxification, and tissue repair5
■ Sirtuin-1 levels, linked to detoxification processes and nutrient sensing6 ■ Number of P16INK4A
positive cells, a biomarker
associated with senescence7 ■ Keratin-14 levels, a biomarker associated with epidermal integrity and renewal8 ■ TNF-α and IL-6 levels, two pro- inflammatory cytokines associated with the inflammatory response and even with chronic inflammation9
Materials and methods Hairy root biomass cultivation and extraction process The cultivation and preparation of hemp root biomass starts with the co-cultivation of Cannabis sativa seeds alongside Agrobacterium rhizogenes wild-type (WT) strains. Following this co-cultivation phase,
Medium Preparation and Addition (water, sugar, vitamins)
individual root clones—distinct segments of the root—are carefully isolated for subsequent growth. These isolated clones are then transferred into a sterile, single-use bag filled with an optimized nutrient-rich growth medium, comprising water, sugars, and essential vitamins to foster hairy root development. The inoculated root clones undergo a four-
week cultivation period within a controlled bioreactor environment. Upon completion of this growth phase, the root biomass is harvested and immediately preserved through vacuum freezing at -20°C, preparing it for the subsequent extraction process. The entire cultivation and preparation workflow is visually summarized in Figure 1. The dried CSHR extract was prepared using
a solvent mixture of ethanol and water in a 50/50 weight ratio. This method was compared to a microwave-assisted extraction process. For the microwave extraction, a vessel connected to a 75-kW microwave generator—operating at frequencies ranging from 0.915 GHz to 2.450 GHz—was utilized. During the process, microwaves and stirring
were applied for 30 minutes. Following this, a solid/liquid separation was performed to isolate the liquid extract. The liquid extract then underwent a series of filtrations using stainless steel mesh to achieve a filtration porosity of 25 micrometers. The final step involved freeze- drying the extract, as illustrated in Figure 2. The CSHR extract was analysed using
liquid chromatography (UHPLC Elute, Bruker), followed by high-resolution mass spectrometry (HRMS, TIMS-ToF). The analysis utilized an HSS T3 column (Waters) maintained at 35°C, with a mobile phase consisting of a gradient of ultrapure water and methanol. The injection volume for the analysis was 1
µL. For detection, a 10 mg sample of the dried extract was dissolved in 10 mL of methanol.
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Co-cultivation of Cannabis s. plantlets & Agrobacterium r. WT strains
Isolation of Root Clones
Inoculation in a single-use bag
Figure 1: Cultivation procedure to obtain Cannabis sativa hairy roots (CSHR)
www.personalcaremagazine.com November 2025 PERSONAL CARE
4 week Bioreactor Cultivation
(biomass growth)
Harvest of Root Biomass
-20°C Vacuum Freezing
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