96 LONGEVITY CONTROL STRESS (UV-A) 0.001% CSHR EXTRACT + STRESS 1% RESVERATROL + STRESS
Sirtuin 1 ■ Nuclear labelling (DAPI) ■ Figure 5: In situ visualization of sirtuin-1 levels. The white dotted line separates the epidermis and the dermis
When analysed by skin compartment, the extract exhibited 93% efficacy in the stratum corneum, 100% efficacy in the epidermis, and 78% efficacy in the dermis.
LAMP2A levels When compared to the control group, the application of the CSHR extract led to a significant 47% increase in LAMP2A levels (Figure 4). This suggests that the extract stimulates autophagy and enhances the detoxification process, potentially contributing to improved skin longevity.
Sirtuin-1 levels Sirtuin-1 levels (visualized in yellow) were detected in situ using epifluorescence
CONTROL
microscopy (Figure 5). Exposure to UVA stress resulted in a reduction of sirtuin-1 levels. However, both the CSHR extract and resveratrol effectively counteracted this stress-induced decrease in sirtuin-1. Compared to the control, UV-A stress caused
a significant reduction in sirtuin-1 levels. The CSHR extract effectively mitigated this decrease, demonstrating an 83% efficacy and suggesting a longevity-protective effect. The positive control, 1% resveratrol, also protected against the UV-A- induced decline in sirtuin-1 levels, achieving a 68% efficacy.
P16INK4A -positive cells
Compared to the control, the UV stress significantly increased the number of P16INK4A
STRESS (UV-A) -
positive cells. On the contrary, the presence of CSHR extract significantly counteracted (with 89% efficacy) the stress-mediated increase in P16INK4A
properties (Figure 6).
Keratin-14 levels In situ analysis of Keratin-14 levels (depicted in purple) was conducted using epifluorescence microscopy (Figure 7). Exposure to UVA stress resulted in a reduction of Keratin-14 levels. However, the application of the CSHR extract effectively reversed this stress-induced decline in Keratin-14. Compared to the control, UV-A stress led
to a significant reduction in Keratin-14 (K-14) levels. The CSHR extract effectively mitigated
0.001% CSHR EXTRACT + STRESS
-positive cells, suggesting anti-senescent
P16INK4a■ Nuclear labelling (DAPI) ■ Figure 6: In-situ visualization of P16INK4A CONTROL -positive cells. The white dotted line separates the epidermis and the dermis STRESS (UV-A) 0.001% CSHR EXTRACT + STRESS
Keratin 14 ■ Nuclear labelling (DAPI) ■ Figure 7: In situ visualization of K-14 levels. The white dotted line separates the epidermis and the dermis PERSONAL CARE November 2025
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