50 ANTI-AGEING


Fibroblast revitalization for dermal regeneration As women notice early signs of ageing, a key concern is the decline in collagen production, which affects skin elasticity and firmness. This endophytic ferment targets fibroblast activity, addressing the root causes of skin ageing to restore its structural integrity and youthful resilience over time.


Youthful skin structure The 0.014% concentration of the active ingredient, which boosts collagen I (+14%*) and elastin (+25%****) formation, along with other activities, demonstrates its effectiveness in promoting well-ageing and skin restructuring.


Anti-glycation capacity Glycation is a reaction in which sugars attach to proteins or lipids, leading to the formation of Advanced Glycation End products (AGEs), which make collagen rigid, reducing elasticity and resistance. AGEs trigger inflammation, causing wrinkles, fine lines, sagging, and impaired wound healing.4,5 A 37%* reduction in AGEs formation was


observed with a 0.0035% concentration of the active ingredient, enabling collagen and elastin quality improvement for an enhanced skin structure.


Cellular regenerative power Ageing impairs the mechanisms of wound healing, leading to reduced cell proliferation, migration, and matrix secretion.6,7 In this context, a 0.014% concentration


of the endophytic ferment boosts fibroblast migration to the wound site (Figure 1) by 77%**, supporting the wound healing process and demonstrating the ingredient’s skin regenerative power.


Anti-wrinkle and smoothing effect Wrinkles are significantly reduced after just 14 days, as shown in vivo profilometry analysis using 2% of the active ingredient. The study, conducted on 76 volunteers,


focused on skin smoothness (Ra & Rz) and wrinkle depth reduction (Sv), confirming that the endophytic ferment is a fast-acting ally against wrinkles. Skin roughness decreased by 8.2%*** in 28 days, while wrinkle depth was reduced by 7.9%** in just 14 days.


UNTREATED SEBOCYTES T-0 T-48h T-48h +77%**Cell Migration Control +0.014% Pureblome


**p<0.01 0.014% Bacillus Ferment vs Control


Figure 1: Optical microscopy imaging to evaluate fibroblast migration. In this assay, cell migration into an exclusion zone was measured. The total area occupied by migrating cells in the detection zone was quantified and expressed as a percentage of the control. The assay was conducted at 0h, 24h, and 48h


Triple reestablishment of skin elasticity When the skin is healthy, its mechanical properties are also optimized. In this regard, 2% of the endophytic active ingredient serves as a powerful ally against age-related elasticity loss. Clinically, it demonstrated significant


improvements compared to placebo in three key elasticity parameters analyzed with Cutometer® after 56 days: -6.5%*** in elastic fatigue (R9), +5.3%**** in elastic recovery (R2), and -4.1%**** in rigid fibre disposition (R0).


Regenerative strength is transferred to the skin Epidermal turnover refers to the process of generating new keratinocytes in the epidermis. The time required for this process to complete is known as the SCTT,7 efficient in acne-prone,8,9


which tends to be less and ageing skin. This


endophytic ingredient enhances skin turnover time by two days, with a reduction in SCTT serving as a clear indicator of its regenerative capacity and a strong demonstration of its well-ageing effectiveness.


Sebocyte rebalancing for refined skin purity As women enter their 30s and 50s, adult acne, often triggered by hormonal changes and lifestyle factors, becomes common. The overproduction of sebum by sebocytes leads to clogged pores and acne.10 This endophytic ingredient targets the


root cause by regulating sebocyte activity, balancing sebum production, and reducing acne-related factors. Decrease in sebum production A significant and dose-dependent decrease in total and neutral lipids (Figure 2) was


SEBOCYTES + LINOLEIC ACID


visually detected and quantified when cultured sebocytes were treated with 0.056% of the ingredient Bacillus Ferment after their lipid production was induced. This reduction in total (-27%*) and neutral


(-21%*) lipids was also observed in vivo, with a significant decrease in sebum production by -16.5%**** after 56 days. These two measurements provide clear and compelling evidence of the anti-acne activity of this endophytic ferment, starting from the roots.


Pore-refining & blemish stopper Potential factors contributing to enlarged skin pores include excessive sebum production and reduced elasticity around the pores.11


Given


that this endophytic ingredient effectively reduces sebum production and enhances skin elasticity, its impact on pore size reduction was remarkably significant after just 14 days of treatment, showing a -5.2%**** average reduction in pore size. This continued to improve further to -10.1%**** at 56 days. Elevated sebum production is a well-


known factor in the pathophysiology of acne.12 However, the most undesirable symptom of this condition is the appearance of acne lesions or blemishes. Therefore, the reduction of both inflammatory and non-inflammatory lesions was also analyzed, resulting in -7.8%* decrease in the average number of blemishes (Figure 3), which also improved further to -10.7** at 56 days.


Mattifying effect and anti-microbial efficacy on oily skin A clinically relevant improvement in matte appearance was observed, based on a defined score by a dermatologically trained expert,


SEBOCYTES + LINOLEIC ACID + 0.056% PUREBLOME (BACILLUS FERMENT)


-27%* -21%*


Total lipids


Neutral lipids *p<0.05 vs Linoleic Acid


Figure 2: Fluorescence microscopy imaging to evaluate lipid production by sebocytes. Sebocyte nuclei are represented in blue. Neutral lipids (originally green) colocalize with total lipids (red), resulting in yellow colour indicating the overlap of both (total + neutral lipids). A significant and dose-dependent decrease in total and neutral lipids was visually detected and quantified after the elicited increase of these lipids by adding Linoleic Acid to the studied sebocyte culture


PERSONAL CARE May 2025 www.personalcaremagazine.com


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