Equine Zygote Development After ICSI 1205
A similar progression of events during zygote development occurred in both groups. IVO when compared with IVM appeared to have a more rapid progression to pronuclei, although some of the potential zygotes were delayed or arrested in development. Abnormal zygote morphologies occurred more frequently in IVM than IVO. Confocal microscopy provided a feasible method to assess zygote development after in vivo or in vitro oocyte maturation using a limited number of samples.
ACKNOWLEDGMENTS
Mare management, oocyte collection, and sample processing was done with the help of staff and students in the Assisted Reproduction Program at the Equine Reproduction Laboratory, Colorado State University (IVO), and ICSI was performed by JoAnne Stokes. The Cecil and Irene Hylton Foundation funded the study. Franc Stone and Abney Foundation scholarships and the Assisted Reproduction Program provided graduate support. Supplemental support came from a core infrastructure grant for microscope ima- ging and R01GM088371 (JGD) at Colorado State University and Ex Ovo Omnia project from Regione Sardegna and Lombardia at Avantea, Laboratory of Reproductive Tech- nologies, Cremona, Italy (C.G.). The supply of ovaries was possible thanks to the collaboration of the Zerbini and Ragazzi slaughterhouse. The help of the technical staff at Avantea is also acknowledged.
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