Effect of melatonin on ram adrenal gland 1181
Fig. 10. Digitally colored transmission electron microscope images showing chromaffin-nerve fibers interaction and Schwann cells in control and treated group. a: Few nerve endings (pink) in control group invaginated the plasma membrane of the chro- maffin cells for a short distance to form small clear synaptic vesi- cles. b: Multiple nerve endings profiles (pink) penetrated the chromaffin cells for a long distance in a typical synaptic type of ending. The nerve endings were surrounded by tubular invagina- tion of chromaffin cells’ plasma membranes (arrows). Both plasma membrane of nerve endings and chromaffin cells approa- ched to form synaptic thickening (arrowhead, inserted figure). c: Schwann cells (SHC) occurred in proximity to nerve fibers in the connective tissue space between the chromaffin cells (CC). It extended tongue-shaped cell processes towards the nerve fibers (NF). d: In treated group, CC were covered externally by large SHC with large nucleus. Bundles of NF containing synaptic vesi- cles, neurotubules, mitochondria, and smooth endoplasmic reticu- lum had been observed lying partly embedded in Schwann cell cytoplasm and made synaptic contact with chromaffin cells (arrow). Note piecemeal degranulation mode of secretion (arrow- heads) of chromaffin cell.
Ganglion cells The control group Ganglion cells were in close contact with chromaffin cells and might be found singly or in small groups. The nucleus was centrally located with peripherally arranged heterochromatin and much amount of euchroma- tin. The cytoplasm contained mitochondria, rER, and ribo- somes (Figs. 11a, 11b). The treated group These cells were characterized by their large diameter and obvious high nucleus to cytoplasmic ratio. The nuclei of these neurons were rounded and the cytoplasm contained numerous rER, free ribosomes, roun- ded and elongated mitochondria, lysosomes and well- developed Golgi apparatus. Many bundles of nerve fibers were observed in close contact with these cells and extended to chromaffin cells (Figs. 11c, 11d).
Fig. 11. Digitally colored transmission electron microscope ima- ges of ganglion cells in control and treated group. a,b: Ganglionic cells (pink) in control group were in close contact with chro- maffin cells (CC). The nucleus (N) was centrally located and their cytoplasm contained mitochondria (M), ribosomes (R). c,d: These cells (pink) were characterized by their large diameter, high nucleus (N) to cytoplasmic ratio, numerous rough endoplasmic reticulum (rER), free ribosomes (R), mitochondria (M), lysosomes
(L), Golgi apparatus (GA), and many bundles of nerve fibers (blue).
The SGC cells. In this study, SGC cells are observed for the first time in the adrenal gland of sheep.
The control group The SGC cells were elongated cells, lying at the periphery of a group of NA-cells, containing a rounded or oval nucleus with peripherally arranged heterochromatin and one nucleolus. SGC cells were connected to typical synaptic-type nerve endings of preganglionic sympathetic axons on their plasmalemma. Dense-cored vesicles were dispersed throughout the SGC cytoplasm among the secre- tory granules. These granules were fewer, smaller (100– 210 nm) than chromaffin cell granules. Electron-dense cores may be found in some vesicles (Fig. 12a). The treated group The SGC cells were characterized by obvious high nucleus to cytoplasm ratio and rich innervation by nerve endings. Co-existence of the dense granules with synaptic-like vesicles (empty or with electron-dense content) that lied in a peripheral position was the most prominent feature of SGC cells, which represent an intermediate position between the chromaffin and sympathetic nerve cells. These adrenal SGC cells were rich in free ribosomes, mitochondria, rER, and multivesicular bodies (Fig. 12b).
Fibroblasts The control group Fibroblasts were observed in the inter- stitial tissue between the chromaffin cells. They had a central indented nucleus, typically euchromatic with a peripheral
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